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The wars in Iraq and Afghanistan required that forensic science was used beyond the traditional law enforcement and criminal justice goals and applied to military operations. The 9/11 terror attacks in the United States (US) inspired further attacks in the Western World and highlighted the importance of national and international intelligence sharing for counterterrorism operations. Following the 9/11 attacks, anthrax was disseminated in the US mail system, demonstrating a successful modern use of biological agents. As a result, nations enhanced their Chemical, Biological, Radiological and Nuclear (CBRN) capabilities to prevent, prepare, respond, and recover from CBRN attacks but in the last decade, there has been a decrease in capabilities across military forensic science, counterterrorism, and CBRN. Recent changes in the global and strategic environment such as, the war in the Ukraine, the threat of grey zone conflicts (below the threshold of war) such as the conflict in the Middle East, and strategic competition, indicates international, national, state and local CBRN capabilities need to be reinvigorated in a world under digital transformations. There is a need for a harmonized approach to enhance the integration of military, law enforcement, and CBRN operations to support decision-making. In this paper, we look back to where we have come from and where we are now to make recommendations on where to from here for military forensic science and counterterrorism, including CBRN which intersects both of these systems., Competing Interests: Declaration of Competing Interest None., (Copyright © 2024. Published by Elsevier B.V.)

Rigorous field assessment in different soil types and climates comparing simulated graves with pig remains and human remains are needed to assess the capabilities and limitations of electrical resistivity tomography (ERT) as a tool to search for unmarked graves. Our study assesses the ERT signals from graves with pig and human remains in a cold, humid continental climate with sandy soils. Two sets of three experimental graves were established: the first set consisted of two graves containing human remains and an empty grave serving as a control, while the second set consisted of two graves with pig remains and a second empty grave. ERT measurements were conducted prior to establishing the graves and were repeated 10 times over seventeen months, except for winter months when measurements were impossible. Each time we acquired eight 18 m long ERT transects using a dipole-dipole electrode array with a unit electrode spacing of 0.5 m and the transects spaced 1 m apart. The measured electrical resistivity decreased for all graves by 14-22 % for measurements conducted up to two months after burial. No further decrease was observed in the control, while resistivity in the graves with human and pig remains continued to decrease by 45-52 % up to the end of our study, seventeen months after burial. The resistivity anomaly in the pig graves shows a contrasting anomaly that is broader than that of the human remains. Our study thus validates the sensitivity of ERT to graves in cold, humid climates with sandy soil., Competing Interests: Declaration of Competing Interest None., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Hyperspectral imaging (HSI) has become a crucial innovation in forensic science, particularly for analysing bodily fluids. This advanced technology captures both spectral and spatial data across a wide spectrum of wavelengths, offering comprehensive insights into the composition and distribution of bodily fluids found at crime scenes. In this review, we delve into the forensic applications of HSI, emphasizing its role in detecting, identifying, and distinguishing various bodily fluids such as blood, saliva, urine, vaginal fluid, semen, and menstrual blood. We examine the benefits of HSI compared to traditional methods, noting its non-destructive approach, high sensitivity, and capability to differentiate fluids even in complex mixtures. Additionally, we discuss recent advancements in HSI technology and their potential to enhance forensic investigations. This review highlights the importance of HSI as a valuable tool in forensic science, opening new pathways for improving the accuracy and efficiency of crime scene analyses., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in the manuscript., (Copyright © 2024 Elsevier B.V. All rights reserved.)

A growing number of studies in forensic voice comparison have explored how elements of phonetic analysis and automatic speaker recognition systems may be integrated for optimal speaker discrimination performance. However, few studies have investigated the evidential value of long-term speech features using forensically-relevant speech data. This paper reports an empirical validation study that assesses the evidential strength of the following long-term features: fundamental frequency (F0), formant distributions, laryngeal voice quality, mel-frequency cepstral coefficients (MFCCs), and combinations thereof. Non-contemporaneous recordings with speech style mismatch from 75 male Australian English speakers were analyzed. Results show that 1) MFCCs outperform long-term acoustic phonetic features; 2) source and filter features do not provide considerably complementary speaker-specific information; and 3) the addition of long-term phonetic features to an MFCCs-based system does not lead to meaningful improvement in system performance. Implications for the complementarity of phonetic analysis and automatic speaker recognition systems are discussed., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Back Ground: The sample collection and preservation before transportation to a Forensic Science Laboratory in a medicolegal case in India is a crucial step in establishing a link between accused, victim, crime scene and the weapon. This paper discusses pioneering advancement in health technology-a state-of-the-art sample dryer developed to preserve biological samples during Forensic investigations. This unique design has been officially registered with the design office in India as a copy right., Aims and Objectives: The primary goal of the sample dryer is to augment the preservation of biological evidence by removing moisture content through air drying before packaging, sealing and its transportation to a Forensic Science Laboratory., Material and Methods: The process of air drying of biological samples by variety of experiments conducted in sample's dryer designed for the purpose which employs a range of air movement techniques including horizontal, vertical, and circular laminar flows to swiftly extract moisture from the samples. The drying duration for each experimental sample was documented upon manual confirmation of complete drying., Results: The results were extraordinary, showcasing that the sample's dryer reduced the drying time drastically by up to 27 times lesser in comparison to conventional methods for identical samples. The diverse airflow patterns generated by the sample dryer unequivocally demonstrated it's utility in achieving superior sample preservation through efficient air drying., Conclusion: Given the exceptional outcomes observed in the experimental phase, the authors strongly advocate for the widespread adoption of this innovative sample's dryer equipment to ensure effective air drying of biological samples and their efficient sample preservation to enable successful forensic analysis as expected., Competing Interests: Declaration of Competing Interest There is no conflict of interest of any kind to declare with regard to the publication of this review article., (Copyright © 2024 Elsevier B.V. All rights reserved.)

F-500 Encapsulator Agent (EA) is a fire suppression agent that is an alternative to traditional firefighting foams. It is marketed as having the capability to act on all four parts of the fire tetrahedron as well as being environmentally friendly and non-toxic. An internal survey of the use of F-500 EA by fire departments encountered by the Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF) ignitable liquid detection canine (ILDC) handlers in 2022 showed that this product is not yet in widespread use across the country, but where it has been implemented, it is frequently utilized on a variety of types of fires. Additional agencies are researching the product to determine if it should be adopted. As this product appears to be growing in popularity, it is important to understand whether the use of the product would affect a canine's ability to detect ignitable liquids or a forensic laboratory's ability to identify the presence of an ignitable liquid. Burned wood and burned carpet, two commonly encountered substrates, were spiked with gasoline or a heavy petroleum distillate (HPD) and F-500 EA was applied. At various time intervals, ILDC teams surveyed the samples and laboratory analysis was conducted. Results showed that the presence of F-500 EA can negatively affect canine alerts and the laboratory's ability to identify ignitable liquids., Competing Interests: Declaration of Competing Interest None., (Published by Elsevier B.V.)

Given that microbiological analysis can be an alternative method that overcomes the shortcomings of traditional forensic technology, and skin samples may be the most common source of cases, the analysis of skin microbiome was investigated in this study. High-throughput sequencing targeting the V3-V4 region of 16S rRNA gene was performed to reveal the skin microbiome of healthy individuals in Guangdong Han. The bacterial diversity of the palm, navel, groin and plantar of the same individual was analyzed. The overall classification based on 16S rRNA gene amplicons revealed that the microbial composition of skin samples from different anatomical parts was different, and the dominant bacterial genus of the navel, plantar, groin and palm skin were dominated by Cutibacterium, Staphylococcus, Corynebacterium and Staphylococcus, respectively. PCoA analysis showed that the skin at these four anatomical locations could only be grouped into three clusters. A predictive model based on random forest algorithm showed the potential to accurately distinguish these four anatomical locations, which indicated that specific bacteria with low abundance were the key taxa. In addition, the skin microbiome in this study is significantly different from the dominant microbiome in saliva and vaginal secretions identified in our previous study, and can be distinguished from these two tissue fluids. In conclusion, the present findings on the community and microbial structure details of the human skin may reveal its potential application value in assessing the location of skin samples and the type of body fluids in forensic medicine., Competing Interests: Declaration of Competing Interest No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Digital transformation rapidly changes how we live our lives in the post pandemic world. Unfortunately, digital technology is not limited to law abiding organisations and citizens. Criminal organisations and individuals are quick to identify new opportunities with new technologies, and digital transformation is dramatically changing the character of crimes, terror, and other threats. The fast emergence of new crimes is facilitated by possibilities brought by disruptive technologies such as AI, Internet of Things, drones, and cryptocurrencies that can be disastrous tools in the hands of criminals. Consequently, our society needs far better capacity to prevent and investigate criminal acts to protect organisations and citizens. This brings an urgent need to proactively reform digital forensics to significantly increase our capability to meet the strain on society brought by crimes evolving in the digital transformation era. The future of forensic science is already here, characterized by a mix of opportunities and challenges. It is essential to make it harder to effectively use digital technologies for criminal activities, while leveraging the possibilities of digital technologies by those affected, law enforcement agencies, business and organisations. As digital technologies continue to evolve, we need to stay up to date with the latest developments to effectively investigate and prosecute crimes in the digital age. There is an increased reliance on digital evidence, and the amount of heterogeneous digital evidence in criminal cases keep increasing. The forensic science techniques thus become more sophisticated and play an increasingly important role. However, the scientific area is extremely broad, and beyond the capability of most forensic science labs to keep up with the technology forefront development speed. Besides an urgent need to bring up the subject to the political arena, examples of how we can meet the challenges are discussed such as by extending our cooperation, encourage and facilitate cooperation for training and education to handle the extremely broad and rapid development, working out methods for explaining and visualising evidence for the treatment and legal values of digital evidence in prosecution, and cooperation between product developers and crime investigators for swift innovation of digital forensics tools and methodologies for quickly emerging threats. This paper will highlight specific examples where modern digital techniques are used to solve crimes in the physical world as well as crimes committed in the digital domain and discuss how "good AI" can be used to fight "evil AI" and finally touch on the sensitive balance between the increased power of the new digital forensic tools and private integrity., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Sexual assault cases typically involve intense physical contact between victim and perpetrator, resulting in a high chance of trace transfer. In addition the victim's, and sometimes the suspect's, declaration is available, and can help direct the forensic investigation. However, many cases are still closed because of insufficient proof or an unknown perpetrator.Previously in Belgium, the forensic analysis strategy was often limited to the detection of human sperm fluid and if not found, the forensic part of the case was closed.Therefore, the Forensic Advice service from the Belgian National Institute for Criminalistics and Criminology (NICC) together with the public prosecutor's office of the Judicial district Antwerp, department Antwerp started "Project code 37". In Belgium every type of crime receives a code and sexual assault cases are categorized under code 37. Because a forensic advisor focuses on the potential of forensic examination on physical objects, the project focused on cases with samples taken on the victim or clothing available.The goal of the project was to perform a contextual analysis in order to go beyond the standard sperm fluid-orientated approach and evaluate if this had a positive impact on the conviction rate. The project also aimed to evaluate the impact of the consistent use of toxicological analyses on conviction rates. Through this systematic contextual analysis, it became clear that four clear categories could be defined: (1) cases with an unknown perpetrator, (2) cases where the suspect denies all contact, (3) cases where the suspect denies (part of) the sexual contact, and (4) cases where only the consent of the victim is disputed. These categories all assumed one female victim and one male perpetrator. Together with the NICC's DNA, trace evidence and toxicology lab, an analysis strategy was drafted for the four categories. All cases that do not fit this description were gathered in a fifth category (custom-made cases), as the victim's/perpetrator's sex has a significant effect on the forensic analysis strategy. At the end of the project, a comparison was made with the same set of cases from another judicial year where the standard analysis strategy was used. For all the categories for which data was available, there was a rise of the conviction rate and a decrease in the number of dismissed cases., Competing Interests: Declaration of Competing Interest I hereby declare that the disclosed information is correct and that no other situation of real, potential or apparent conflict of interest is known to me., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Franz Kafka had beautiful eyes. So striking, that many of the famous author's friends and peers commented on them - but quite variously ('dark', 'brown', 'grey' & 'blue'). Eye colour as perceived by an observer is subjective, being influenced by physiological, environmental, and even sociocultural factors. In a policing context, this does not mean that trait information such as eye colour is not valuable (far from it), but that it must be managed carefully. The Australian Federal Police has recently implemented a forensic DNA phenotyping (FDP, aka. physical trait prediction or PTP) capability, utilising massively parallel sequencing DNA technology to predict an individual's eye colour, biogeographical ancestry and sex from a crime scene sample. This information alone is not itself 'intelligence', but can be used to generate intelligence through holistic analyses undertaken within a transdisciplinary, all-source forensic intelligence (FORINT) framework. FORINT outputs posit abductive propositions typically at the activity/offence level, to provide insight and influence decision making. However, the use of predicted traits requires that they are compared to something; all Australian police databases include fields for physical traits, but no uniform standard is applied across all agencies. Moreover, collection is inconsistent and no automated systems are in place to capture such data systematically. Consider the 'Kafka problem': his peers gave multiply divergent descriptions of his eyes. If a Biology unit had predicted the eye colour of an 'unidentified author' using DNA - how would Kafka be confidently nominated as the contributor? We posit three maxims for law enforcement: (1) To expand the operational utility of forensic science in line with police demands, forensic science should operationalise FDP (e.g. operationally to rank a list of persons of interest, focus lines of enquiry in serious & organised crime, or assist with human remains identification). (2) Such advanced biological techniques are best delivered through an all-source FORINT framework, to maximise opportunities and minimise risk. (3) One cannot pursue techno-scientific advancements in isolation; it is also necessary to influence the operational posture for their implementation. In this paper we explore these issues and provide recommendations relating to (a) police practices, (b) image capture systems, and (c) research opportunities. Phenotypic trait prediction has great potential and can be operationalised effectively through a rigorous FORINT framework. However, there is (continual) work to be done to enhance the operational capabilities that are complementary to - but necessary for - effective forensic science contribution to investigations., Competing Interests: Declaration of Competing Interest None., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The natural occurrence of 16 inorganic ions relevant to forensic explosives investigations on human hands was studied to support the evaluation of activity-level propositions when such traces are found on the hands or in the fingerprints of a suspect. A total of 594 hand swab extracts from 297 participants throughout Europe and the United States of America were analyzed using Ion Chromatography - Mass Spectrometry. The data provides a reference framework for future covert investigations and forensic casework. The results indicate that thiocyanate, chlorate, nitrite, lithium, strontium, and barium are rarely detected on the hands of individuals who have had no direct contact with explosives (P<0.03) and in quantities below 6 µg. Perchlorate contamination sporadically occurs without deliberately handling perchlorates (P=0.03), albeit at low levels (<12 µg). It also seems that the presence of perchlorate on hands is generally related to professions that involve explosives. Detecting substantial amounts of any of these rare ions on a suspect's hands would require a specific explanation. Because legitimate activities exist that can also result in elevated levels of ions of interest on hands, the context surrounding their presence has to be carefully assessed for each individual case., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Cigarettes are part of a collection of objects found everywhere and smoked by a large part of the population. Cigarette butts can be an essential piece of evidence in identifying a certain suspect/witness, as they can suggest the positive identification or exclusion of one or more brands by comparison or even DNA analysis. The main objective of this study is to test the capability of Handheld X-ray fluorescence spectrometer (HHXRF) to analyze the elemental concentration of individual cigarette ash of several tobacco brands and investigate if it is further possible to discriminate the different brands based on their ash's elemental concentration. This study reveals the capability of HHXRF to discriminate tobacco brands based on their ashes' elemental concentration, with the great advantage of the analyses being non-destructive and can be carried out on a small sample. In addition, this equipment can measure the ash's elemental concentrations on-site, allowing for less contamination and sample loss., Competing Interests: Declaration of Competing Interest The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties. No writing assistance was utilized in the production of this manuscript., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Forensic reconstruction and scenario evaluation are crucial in investigations of suspicious deaths related to falls from a height. In such cases, distinguishing between accidental falls, being pushed or jumping is an important but difficult task, since objective methods to do so are currently lacking. This paper explores the possibility of repurposing a passive rigid body model of a human from commercially available crash simulation software for forensic reconstruction and scenario evaluation of humans dropping from heights. To use this approach, a prerequisite is that the human body model can produce realistic movements compared to those of a real human, given similar environmental conditions. Therefore, this study assessed the validity of the commercially available Simcenter Madymo Pedestrian Model (MPM) for simulating human fall movements. Experimental kinematic and kinetic data was collected from nine participants, who dropped from a height in three different ways: passively tilting over, getting pushed, and jumping. Next, the performance of the MPM in reproducing the kinematics of the experimental falls was assessed by comparing the orientation of the body 0.3 s after platform release. The results show that the MPM currently does not consistently reproduce the experimentally recorded falling movements across multiple falling conditions and outcome measures. The MPM must therefore be adapted if to be used for forensic reconstruction and scenario evaluation, for example by implementing active movement., Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Expert testimony is only admissible in common-law systems if it will potentially assist the trier of fact. In order for a forensic-voice-comparison expert's testimony to assist a trier of fact, the expert's forensic voice comparison should be more accurate than the trier of fact's speaker identification. "Speaker identification in courtroom contexts - Part I" addressed the question of whether speaker identification by an individual lay listener (such as a judge) would be more or less accurate than the output of a forensic-voice-comparison system that is based on state-of-the-art automatic-speaker-recognition technology. The present paper addresses the question of whether speaker identification by a group of collaborating lay listeners (such as a jury) would be more or less accurate than the output of such a forensic-voice-comparison system. As members of collaborating groups, participants listen to pairs of recordings reflecting the conditions of the questioned- and known-speaker recordings in an actual case, confer, and make a probabilistic consensus judgement on each pair of recordings. The present paper also compares group-consensus responses with "wisdom of the crowd" which uses the average of the responses from multiple independent individual listeners., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr Morrison is Director and Forensic Consultant for Forensic Evaluation Ltd. Dr Weber has worked as a contractor for Forensic Evaluation Ltd. Forensic Evaluation Ltd charges clients fees to perform forensic-voice-comparison evaluations, and to submit reports and testify in court regarding forensic voice comparison, and regarding speaker recognition and speaker identification by laypersons., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

This paper introduces the concept of an operational reference glass database, specifically designed for broken glass fragments from ATM attacks, jewelry store robberies, and ramraids on high-end clothing stores. The database, initiated in 2014, is used to compare glass traces from organized crews involved said criminal activities. Utilizing LA-ICPMS, this study establishes a methodology for collecting reference glass samples from the scenes of the aforementioned crimes, thus creating a comprehensive database containing over 3500 reference glass samples from crime scenes. The operational database is employed to match trace elemental profiles of glass fragments from suspected items to known reference samples, offering specificity and accuracy. Analysis of results, while ongoing due to the nature of active cases, find matches of trace materials in over 50 % of case requests since 2019. Challenges such as database scalability and continuous updating are acknowledged, and future directions include technological advancements to enhance precision and the application into other areas of forensic material analysis. The paper emphasizes the efficacy of this specialized approach in chemical profiling, providing a potent tool for linking glass traces to specific criminal contexts and providing intelligence and investigative leads into individuals involved in ATM-related crimes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Detecting DeepFake videos has become a central task in modern multimedia forensics applications. This article presents a method to detect face swapped videos when the portrayed person in the video is known. We propose using a threshold classifier based on similarity scores obtained from a Deep Convolutional Neural Network (DCNN) trained for facial recognition. We compute a set of similarity scores between faces extracted from questioned videos and reference materials of the person depicted. We use the highest score to classify the questioned videos as authentic or fake, depending on the threshold chosen. We validate our method on the Celeb-DF (v2) dataset (Li et al., 2020) [13]. Using the training and testing splits specified on the dataset, we obtained an HTER of 0.020 and an AUC of 0.994, surpassing the most robust approaches against this dataset (Tran et al., 2021) [37]. Additionally, a logistic regression model was used to convert the highest score into a likelihood ratio for greater applicability in forensic analyses., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

We review the application of Nuclear Analytical Techniques (NATs) to forensic problems for the first time. NATs include neutron activation analysis (NAA), carried out in nuclear reactors for elemental analysis; accelerator-based techniques, mainly Ion Beam Analysis (IBA) for elemental and molecular analysis; and Accelerator Mass Spectrometry (AMS) for dating of traces of forensic interest by "radiocarbon dating" and other related methods. Applications include analysis of drugs of abuse, food fraud, counterfeit medicine, gunshot residue, glass fragments, forgery of art objects and documents, and human material. In some applications only the NATs are able to provide relevant information for forensic purposes. This review not only includes a wide collection of forensic applications, but also illustrates the wide availability worldwide of NATs, opening up opportunities for an increased use of NATs in routine forensic casework., Competing Interests: Declaration of Competing Interest The authors declare they have no conflicts of interest., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

While plant species identification in forensics can be useful in cases involving poisonous, psychoactive, or endangered plant species, it can also become quite challenging, especially, when dealing with processed, decaying, colonized or infected material of plant origin. The Animal Plant and Soil Traces expert working group of the European Network of Forensic Science Institutes in their best practice manual has recommended several markers for plant species identification. Current study is a part of implementation of method in a forensic laboratory and its aim is to evaluate four of the recommended markers (ITS, matK, rbcL, and trnH-psbA) for species identification of forensically important plant species including medicinal, poisonous, psychoactive, and other plants. Such parameters as PCR and sequencing success, sequence length, species resolution rate and species cover in GenBank were analysed. Blind testing was performed to evaluate use of the markers for identification of forensically more complicated samples. According to results, a combination of ITS, matK and trnH-psbA is the best choice for plant species identification. The best results with fresh plant material can be achieved with ITS, trnH-psbA, and matK, while ITS and matK are the best choice when working with low quality plant material. rbcL due to its low species discrimination rate can be used only as an indicative marker., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Tire impression evidence can be a valuable tool during a crime scene investigation-it can link vehicles to scenes or secondary locations, and reveal information about the series of events surrounding a crime. The interpretation of tire impression evidence relies on the expertise of forensic tire examiners. To date, there have not been any studies published that empirically evaluate the accuracy and reproducibility of decisions made by tire impression examiners. This paper presents the results of a study in which 17 tire impression examiners and trainees conducted 238 comparisons on 77 distinct questioned impression-known tire comparison sets (QKsets). This study was conducted digitally and addressed examinations based solely upon the characteristics of the tire impression images provided. The quality and characteristics of the impressions were selected to be broadly representative of those encountered in casework. Participants reported their decisions using a multi-level conclusion scale: 68% of responses were class associations (Association of Class Characteristics or Limited Association of Class), 21% were definitive decisions (ID or Exclusion), 8% were probable decisions (High Degree of Association or Indications of Non-Association), and 3% were neutral responses (Not Suitable or Inconclusive). Although class associations were the most reported response type, when definitive decisions were reported, they were often correct: 96% of IDs and 89% of Exclusions were consistent with ground truth regarding the source of the known tire in the QKset. Overall, we observed 4 erroneous definitive decisions (3 Exclusions on mated QKsets; 1 ID on a nonmated QKset) and 1 incorrect probable decision (Indications of Non-Association on a mated QKset). Decision rates were notably associated with both quality (lower quality questioned impressions were more likely to result in class associations) and dimensionality (2D questioned impressions were more likely to result in definitive decisions), which were correlated factors. Although the study size limits the precision of the measured rates, the results of this study remain valuable to the forensic science and legal communities and provide empirical data regarding examiner performance for a discipline that previously did not have any such estimates., Competing Interests: Declaration of Competing Interest Authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In Colombia there are estimated to be over 121,000 missing people and victims of forced disappearances. Forensic investigators therefore need assistance in determining optimal detection techniques for buried victims, to give victims' families closure and for the wider community to see that justice is being served. Previous research has created 12 controlled simulated clandestine graves of typical Colombian murder victim scenarios at 0.5 m - 1.2 m depths in savannah and rainforest sites in Colombia. The 0-3 years of geophysical monitoring results of were published, with this paper reporting on 4-8 years monitoring of both UAV drone results and geophysical data. The UAV results from the year 8 survey, published for first time from Colombia, showed that the simulated graves could still be located using NDVI and NIR multi-spectral data, but not using optical or other multi-spectral data. The 0-3 years of geophysical data found the simulated clandestine graves could be detected with electrical resistivity and GPR methods, with the 4-8 year surveys evidencing that they could still be detected using bulk ground conductivity surveys, GPR horizontal time slice datasets and 2D ERT profiles. Research implications suggest initial use of UAV remote sensing technology to pinpoint likely search areas, before subsequent ground reconnaissance, geophysical surveys and their interpretation, before intrusive investigation methods are employed for detecting missing and disappeared persons in Colombia., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Forensic facial image comparison based on recognition algorithms has been widely applied in forensic science. Previous researches have been concentrating on the cases of using single system during comparison, while how to use multiple systems has not yet been studied. In this paper, a dual-systems model (including SeetaFace and FaceNet) for facial comparison was constructed, and Bayesian networks were utilized as the basic frame. In order to prove its superiority, a large-scale experiment (on the dataset CelebA) has been carried on to evaluate the score-based likelihood ratio. We used three likelihood ratio evaluation tools (Empirical Cross-Entropy, Cost Likelihood Ratio, Limit Tippett Plots) to assess the performance of the model. The Wasserstein distance was also used to evaluate the detailed likelihood ratio performance. The experimental results show that the likelihood ratio performance of our dual-systems model is better than single system. Besides, our method of model building and evaluation can also be used in the condition of triple or more systems., Competing Interests: Declaration of Competing Interest All authors disclosed no relevant relationships., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Wildlife forensics is defined as providing forensic evidence to support legal investigations involving wildlife crime, such as the trafficking and poaching of animals and/ or their goods. While wildlife forensics is an underexplored field of science, the ramifications of poaching can be catastrophic. The consequences of wildlife crime include disease spread, species and habitat loss, human injury, and cultural loss. Efforts to use forensic science to combat poaching are currently limited to DNA-based techniques. However, fingermark analysis for the identification of perpetrators of wildlife crimes has not been explored to the same extent, despite being a cost-effective, simple-to-use forensic method that is easy to deploy in-field. This review covers literature that has explored fingermark examination techniques used on wildlife-related samples, such as pangolin scales, ivory-based substances, bone, and eggs, as well as feathers and skins, among more obscure trafficked items. Useful preliminary work has been conducted in this subject area, demonstrating that commonly used fingermark analysis techniques can be applied to wildlife-based items. However, many of these studies suffer from limitations in terms of experimental design. More work should be done on creating studies with larger sample sizes and novel approaches should be validated under environmental conditions that mimic real crime scenes. Further research into determining the forensic fingermark analysis techniques that perform the most efficiently in the environmental conditions of the countries where they are needed would therefore benefit legal investigations and help to reduce instances of poaching., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Further steps toward understanding the time-related information contained within bloodstains found at the crime scene are rightly considered a top priority in forensic science. Contrary to widely held assumptions, the reason for the delayed exploitation of bloodstains dating methods in practice is not the lack of suitable analytical techniques for monitoring degradation processes. The problem lies in the variability of the environmental and circumstantial conditions, playing a vital role in the degradation kinetics of blood deposits. The present article demonstrates the possibility of breaking with current approaches based on absolute age estimations to finally answer time-centered questions in real forensic scenarios. The proposed novel framework for situating forensic traces in time is based on the likelihood ratio assessment of the (dis)similarity between the evidence decomposition and sets of reference materials obtained through supervised aging. In such a strategy, every dating procedure is constructed on a case-by-case basis to fit examined blood traces, thereby limiting the adverse influence of external factors on the validity of age estimations and providing a way for future crime scene implementation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Many different issues have been identified in forensic science for more than 10 years. While quality management has often been suggested as a path forward, research is generally considered as an essential part of the solution. Through an overview of current forensic science research, this paper aims at evaluating if and how research answer the challenges forensic science is currently facing. While forensic related publications have massively increased over the years, approximately half of the publications were published in non-forensic sources, indicating that forensic science research tends to be led by other disciplines. Over the years, forensic science research has remained largely oriented towards methodological and technological development rather than relevance to the forensic science discipline and practice. Practical implementation of the techniques is rarely discussed from a forensic perspective, and thus research rarely move from the "proof-of-concept" stage to its utilisation in case investigation. The digital transformation also generated a massive increase of data, making it challenging to find the relevant pieces of information in the mass of "forensic" publications available on-line. Thus, we propose to refocus forensic science research on forensic fundamental and practical questions to strengthen the discipline and its impact on crime investigation and security issues. Our propositions represent an incentive to further discuss forensic science research and knowledge transmission through the definition of a common culture within the community, focusing on common fundamental knowledge such as a better understanding of the concept of trace and its case-based information content., Competing Interests: Conflict of interests none., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Diatoms (Bacillariophyta) are unicellular photosynthetic organisms commonly occurring in aquatic habitats on Earth. Their autecology makes them almost perfect indicators of environmental conditions and so have high potential for use in forensics. Both eurytopic and stenotopic species are important in site identification: eurytopic forms due to their abundance, and stenotopic forms due to their narrow range of tolerance to environmental conditions. Their presence can hence provide a clear insight into an ecosystem and its microhabitats. The diatoms are useful as significant indicator in diagnosing of drowning. However, to definitively link a corpse with the place where it was found and to indicate whether this was also the crime scene, it is essential that any comparative diatomaceous analyses are performed correctly. The following study presents selected cases in which a comparative diatomaceous analysis was performed. In all cases, the biological samples secured during the autopsy were compared with environmental samples collected from the site of the cadaver disclosure. Our findings show both the possibilities and limitations of using this method in forensics. These forensic investigations need close collaboration between coroners and diatomology experts. It is crucially important to reveal whether the place of corpse finding is the same as the place of drowning., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Forensic facial image comparison lacks a methodological standardization and empirical validation. We aim to address this problem by assessing the potential of machine learning to support the human expert in the courtroom. To yield valid evidence in court, decision making systems for facial image comparison should not only be accurate, they should also provide a calibrated confidence measure. This confidence is best conveyed using a score-based likelihood ratio. In this study we compare the performance of different calibrations for such scores. The score, either a distance or a similarity, is converted to a likelihood ratio using three types of calibration following similar techniques as applied in forensic fields such as speaker comparison and DNA matching, but which have not yet been tested in facial image comparison. The calibration types tested are: naive, quality score based on typicality, and feature-based. As transparency is essential in forensics, we focus on state-of-the-art open software and study their power compared to a state-of-the-art commercial system. With the European Network of Forensic Science Institutes (ENFSI) Proficiency tests as benchmark, calibration results on three public databases namely Labeled Faces in the Wild, SC Face and ForenFace show that both quality score and feature based calibration outperform naive calibration. Overall, the commercial system outperforms open software when evaluating these Likelihood Ratios. In general, we conclude that calibration implemented before likelihood ratio estimation is recommended. Furthermore, in terms of performance the commercial system is preferred over open software. As open software is more transparent, more research on open software is urged for., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The development of fingermarks on reflective surfaces is often a challenge regarding the photography of images with overlapping lines, low contrast and reflections, especially considering that many forensic laboratories are supplied only with basic instrumentation for fingerprint analysis. The present study overviews these difficulties and proposes a combination of chemical and optical procedures, using low-cost products and equipment, to develop fingermarks on silver mirror surfaces. The chemical treatment promotes the delimitation of the substrate, transforming the reflective surface into a transparent surface. The results were statistically analyzed, indicating quality improvement of natural fingermarks pictures taken with standard digital camera on transparent surface. There was good observation of details and minutiae, even for samples recovered several days or weeks after deposition. The suggested method substantially modifies the composition of the substrate without any contact with the fingermark, preserving its characteristics and properties. Like other nondestructive methodologies, this approach could be prioritized over methods that directly change the evidence itself and allows for the photography of the fingermark in unaltered condition. Lastly, it does not impact on the efficiency of subsequent exams., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Multi-metal deposition (MMD) is a versatile fingermarks detection technique adapted from the colloidal gold biolabeling. However, the tedious procedures of MMD makes it receive little attention compared with other methods. The aim of this study is to evaluate the efficacy of MMD technique on several common fabrics, which is considered notoriously challenging for latent fingermark detection. Four different MMD formulations were examined to process fingermarks deposited on nylon taffeta, polyester taffeta, polyester pongee and cotton sateen to determine the most suitable one and the influence of aging and water immersion were also determined through subsequent experiments. It was found that MMD I outperformed other three formulations and obtained excellent results on nylon taffeta, polyester taffeta and satin ribbon, with polyester taffeta and satin ribbon providing more than 30% of identifiable marks even for fingermarks aged over 28 days. Cotton sateen and oxford cloth failed to produce ridge details but evidence of "touch" were successfully visualized, which may contribute to further DNA extraction. Water immersion did have some observable influence on the quality of detected marks as part of the MMD reactant within fingermarks lost during immersion, but the result from nylon taffeta and satin ribbon is still satisfying with the percentage of marks scored 3 and 4 reached 30%. The result of this study confirmed the capability of MMD I in treated with fingermarks on several kinds of fabrics, and shows potential to promote this non-instrumentation dependent technique., Competing Interests: Conflict of interest The authors declare that they do not have any competing interest to declare. There is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Visualization of latent fingermarks on light fabric using multi-metal deposition (MMD)---A preliminary study”., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Being able to identify the author of an anonymous or disputed document is an important task in forensic science. This can be treated as a form of pattern evidence based on writing style, but the subjective analysis of writing style may have all the well-known problems of other forms of subjective pattern evidence. In this paper, we demonstrate a computer program to address these issues. This program analyzes a pair of documents (a known document and a questioned document) to determine if they were written by the same author. More importantly, this paper also validates the accuracy of this program through a large-scale series of controlled experiments involving English language blogs. Across more than 32,000 different document pairs, the system achieved a measured accuracy of 77%. This paper concludes that this system not only addresses a key problem in forensic linguistics, but also provides the repeatability, reproducibility, and measured accuracy levels that are key to the advancement of forensic science., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Ground-penetrating radar (GPR) is an established geophysical technique used extensively for the accurate reconstruction of the shallow (<10 m) subsurface. Reconstructions have largely been completed and presented as 2D vertical and horizontal planes, leaving limited visualization of subsurface 3D shapes and their spatial relationships. With technological advancements, particularly the availability and integration of various software platforms, 3D modelling of GPR data is now emerging as the new standard. However, despite these developments, there remains an inadequate examination and testing of these techniques, particularly in determining if their application is beneficial and warranted. In this study we conducted a GPR grid survey on a churchyard cemetery to generate and evaluate 2D and 3D-modelled reconstructions of the cemetery burial sites. Data collection and processing was completed using a Sensors and Software Incorporated pulseEKKO™ Pro SmartCart GPR system and EKKO&#95;Project™ software, respectively. The modelling component was achieved using Schlumberger's Petrel™ E & P software platform, which is tailored to the petroleum industry. The subsurface patterns present in the 2D and 3D models closely matched the cemetery plot plan, validating our data collection, processing, and modelling methods. Both models were adequate for 2D horizontal visualization of reflection patterns at any specific depth. The 3D model was used to identify the presence of a companion burial plot (stacked caskets) and possible leachate plumes below and encircling burial sites, both of which were not evident in the 2D model, highlighting the benefits of 3D modelling when discerning subsurface objects. We expect our findings to be of value to similar GPR studies, with particular significance to geoforensic studies and criminal investigations., (Copyright © 2021. Published by Elsevier B.V.)

Bidis are small handmade cigarettes consisting of ~0.2 g of tobacco flakes rolled in a dried leaf of 'Tendu' (Diospyros melanoxylon) or Piliostigma racemosum and tied with a thin thread. They have gained worldwide popularity among smokers and are often collected as forensic DNA evidence from crime scenes and processed similarly to cigarette butts. However, bidi's composition and manufacturing process differs distinctly from the cigarette, and hence the optimal processing for DNA analysis should not be assumed to be similar to cigarette butts. In the present study, the methodical evaluation of the bidi for DNA analysis is reported and an additional process of cell elution from bidi stubs prior to DNA extraction is systematically compared with the direct lysis of bidi stubs which is identical to the standard practice in forensic labs for cigarette butts. In terms of cell recovery from bidi stubs, the SDS based Cell Elution Buffer (CEB) proved to be better than Tween20 based CEB. The three components (cell-elute, supernatant, and processed stub) obtained after the cell elution process of smoked bidi stubs showed varying amounts of DNA. The cell-elute and processed stub exhibited high quality DNA, resulting in 90-100% of the samples giving a full STR profile. On the contrary, the directly lysed stubs yielded high quantity but low quality of DNA, resulting in only 40% of the samples with full STR profiles. The cell elution process enables three components namely cell-elute, supernatant and processed stub from the same bidi to be used for forensic DNA analysis, although the cell-elute proved to be the best source of DNA for STR profiling. The current study demonstrates that the additional cell elution process proved to be an essential step prior to DNA extraction procedure for bidi stubs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The purpose of this study was to compare the accuracy and precision between the Reverse Projection and PhotoModeler methods for suspect height analysis. Thirty analysts were assigned to measure the heights of three different suspects, one for each method, with the suspects having been recorded standing at three different distances in a scene. For Reverse Projection, the analysts were provided with height scales to place and video-record at the same positions their suspects stood in at the test scene, so that frames could be extracted from the video and overlaid onto frames of the suspects to measure height. For PhotoModeler, analysts calibrated frames of the suspects using 3D point cloud data obtained from a laser scan of the scene, so that measurements could be made in PhotoModeler software. Errors were calculated for the measurements and compared using the Mann-Whitney U-test and Kruskal-Wallis H-test, which indicated significant differences for errors between the two methods (p = 0.0025 and p = 0.008). Reverse Projection yielded a greater range of error and tended to have higher standard deviations than PhotoModeler, but the overall accuracy between the two methods was found to be comparable. The majority of absolute measurement errors for both methods were less than 2 cm., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The sensitivity of imaging spectroscopy to haemoglobin derivatives makes it a promising tool for detecting blood. However, due to complexity and high dimensionality of hyperspectral images, the development of hyperspectral blood detection algorithms is challenging. To facilitate their development, we present a new hyperspectral blood detection dataset. This dataset, published under an open access license, consists of multiple detection scenarios with varying levels of complexity. It allows to test the performance of Machine Learning methods in relation to different acquisition environments, types of background, age of blood and presence of other blood-like substances. We have explored the dataset with blood detection experiments, for which we have used a hyperspectral target detection algorithm based on the well-known Matched Filter detector. Our results and their discussion highlight the challenges of blood detection in hyperspectral data and form a reference for further works., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Bayesian networks have shown to be a useful tool for the evaluation of forensic findings given activity level propositions. In this paper, we demonstrate how case specific experiments can be used to assign probabilities to the states of the nodes of a Bayesian network for the evaluation of fingermarks given activity level propositions. The transfer, persistence and recovery of fingermarks on knives is studied in experiments where a knife is either used to stab a victim or to cut food, representing the activities that were disputed in the case of the murder of Meredith Kercher. Two Bayesian networks are constructed, exploring the effect of different uses of the experimental data by assigning the probabilities based on the results of the experiments. The evaluation of the findings using the Bayesian networks demonstrates the potential for fingermarks in addressing activity level propositions., Competing Interests: Declaration of Competing Interest None., (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)

Considering the widespread use of mobile phones, audio recordings of crime scenes are widely used as digital evidence; however, it is important to authenticate the audio recordings before consideration as legal evidence. This study aimed to develop a method to authenticate audio recordings generated using the iPhone through three steps: 1) bitrate/audio latency time analysis of audio recordings, 2) comparison of the file structure/timestamp on audio recordings, and 3) device-based log history examinations for the provenance of audio recordings. Herein, we analyzed audio recording samples from ten different models of mobile handsets of the iPhone with Advanced Audio Coding (AAC) or Apple Lossless Audio Codec (ALAC), through the Voice Memos application depending on the iPhone Operating System (iOS). To analyze the characteristics of these audio recordings, we compared features including audio latency, file format/structure, and timestamps between the audio recordings generated in the iPhone and those edited through the built-in audio editing function. Furthermore, we investigated the log history registered in devices during the generation of the audio recordings. Differences in the audio latency, file size, timestamps, bitrate, and log history were confirmed on the iPhone when manipulating the audio recordings. The present results show that it is possible to verify the authentication of audio recordings generated using the Voice Memos application on iPhone., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The estimation of the time since death (minimum Post Mortem Interval, minPMI) is an essential aspect of forensic investigations. This is particularly complex when a human body is found submerged, floating or beached in a marine environment. When a cadaver is found in a terrestrial environment the minPMI estimation is generally based on the presence of carrion insects. However, when a cadaver is found in an aquatic environment, a correct crime scene reconstruction is more complex and requires the consideration of the time the remains spent submerged underwater (minimum Post Mortem Submersion Interval, minPMSI) and/or floating (Floating Interval, FI). In marine crime scene scenarios, the use of barnacles (Crustacea: Cirripedia) has recently received some attention, due to their permanent settlement on human remains and their accompanying clothing. Previous research considered barnacle growth on human shoes, but the present research is the first to focus on the colonisation of barnacles on clothing materials (fabrics). Polystyrene floats were covered by either cotton, velvet, satin or neoprene and submerged underwater over a period of six months off the coast of Perth, Western Australia. The aims of this research were 1) the identification of marine species colonising the fabrics, with special attention to barnacles; 2) the identification of which fabric type provides the most desirable environment for colonisation; and 3) the identification of factors that affect the growth rate of the different species. Three species of barnacles, Balanus trigonus Darwin, Amphibalanus reticulatus (Utinomi) and A. variegatus (Darwin), were present in varying numbers and sizes. The colonisation process of the barnacles occurred rapidly, with the first sighting of barnacles observed within the first month on neoprene and control floats. The surface that attracted the largest number of barnacles was neoprene, followed by satin and cotton, while velvet showed an inconsistent colonisation rate. The largest size barnacles were observed on the control floats, while all fabrics showed a similar smaller size. Overall, time spent in water and water temperature had a significant positive relationship with both number and size of the colonising barnacles. This study is the first to provide information that will aid in the investigation of human remains recovered from Western Australian marine waters, using the barnacle colonisation on different fabric types., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Plant science has been more and more utilized in forensic investigation, although its full potential is still to be reached. Plant macroremains are a powerful tool to link a body or other evidence back to a primary crime scene as they can provide detailed information about its previous ecological and geographic location. However, plant macroremains are often poorly preserved and difficult to identify, as diagnostic elements are seldom present within the assemblage occurring on the scene. Plant fragments most likely to be found are those exposed to the environment and resistant to degradation. The bark of woody plants meets these requirements but the possibility of its identification at species level from small fragments is not known. Starting from a real homicide case, where bark splinters were found on the victim, we aimed to assess the forensic potential of bark identification from small fragments like those likely to occur on a crime scene. Two identification keys were prepared for 16 common lowland tree species from Northern Italy; one key used all the available anatomical traits, the second only those from the outer bark. The second key was not able to discriminate some couples of species unambiguously, but could identify the bark fragments of the homicide as Robinia pseudoacacia, as confirmed from direct comparison with a reference sample. Bark fragments deserve to be included into the macroremains to be analyzed during an investigation, but small samples could easily lack diagnostic traits, and the building of a reference collection should be encouraged., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Textile structures are considered as the main component of protective clothing against the penetration of sharp objects. In the current research work, woven fabrics reinforced with metal threads as weft yarn were designed with various weave patterns including Twill, Satin, and Hopsack, and by using a weft-backed weaving structure. The mentioned reinforced samples were produced by altering the diameter and number of metal threads in each fabric structure. Assessment of fabric resistance against bending and sharp object penetration reveals that although the application of metal thread improves the fabric resistance against sharp object penetration, it leads to an increase in the fabric stiffness. According to the results, the influence of the number of metal threads on the penetration resistance was considerably higher than the metal thread diameter. However, more increase in the fabric's bending stiffness was observed with the rise of metal thread's diameter compared to the number of metal threads. According to the obtained outcomes, weave structure is an effective parameter that changes fabric's resistance against penetration. In this regard, weave patterns with more firmness that limit the yarn movement during the indentation of the sharp object present higher protection ability. Furthermore, the increment of fabric density enhances the resistance against penetration. In this study, the resistance of two-layer samples with various orientations angle of 0 ° /90 ° and -45 ° /+45 ° were probed, as well. Outcomes confirmed that two-layer samples resist better than single-layer sample against sharp object penetration due to the formation of a grid of metal threads. Among two studied fabrics' orientation angles, the sample with the angle of -45 ° /+45 ° presents greater penetration resistance., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Wildlife trade and fraudulence in food, artefacts and cosmetic industries had raised serious concern in protection of the wild faunal diversity. Lack of proper tools and molecular based techniques for identification of wild species are some of the major constrains faced by the judiciary and law enforcement agencies while framing charges against poachers and illicit agitator. The emergence of wildlife forensics serves as a boon in solving long pending cases of wildlife crimes. Wildlife forensics have proven to be fast, accurate and reliable criminal investigation processes with comprehensive coverage and easy accessibility. It has also helped resolving taxonomic disputes, determining spatiotemporal genetic divergence, evolutionary history, origins and even endemism. Collaboration among inter-disciplinary fields has even led to engineered signature markers and phylogenetics for several species. Development in fields of genetics, molecular and evolutionary biology and other omics techniques have further contributed in accurate identification of species. Wildlife forensics, with the support of proper international mega database units for population reference, will be fundamental in wildlife investigations through its unlimited information sharing ability. The efficient conservation of species will, however, require a collaborative approach consisting of national policy makers, local stakeholders and implementation agencies in addition to experts from the scientific communities., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

A taphonomic research facility for the study of human remains was recently realized in Amsterdam, the Netherlands, to systematically investigate the decomposition of the human body under known conditions. Governmental authorization was obtained to make use of the body donation program of the Amsterdam University Medical Centers, location Academic Medical Center, for this specific purpose. In contrast to the small number of comparable initiatives elsewhere, this facility specifically allows for the study of buried bodies e.g. with the use of telemetry and remote sensing. Here, we discuss the concept of body donation in the Netherlands, its role in taphonomic research, and the sequence of events that preceded the realization of this facility, which is the first of its kind in Europe. In addition to offering novel research options to the scientific community, we hope that it will also pave the way for the successful realization of similar initiatives in other locations., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)

The effectiveness of alternate light source (ALS) to fluoresce bone and other materials is well-attested to in a laboratory setting but rarely, if ever, has it been used in field excavation. This study examined the recovery rates of fragmentary bone, fabric, and metal, both with and without the use of an ALS, through practical and controlled excavation experiments with multiple users. All archaeology, including forensic archaeology and crime scene investigation more generally, should account for trace evidence. Currently, there is limited empirical data for the recovery of evidence from excavation, and those studies that do exist, highlight the short-comings in current methods. Six comparable test pits were created, representing empty graves in which only trace evidence remained. Each contained 20 fragments of bone (≤10mm), 16 hair fibres, two pieces of fabric and two lead pieces, which were back-filled and left for over 15 weeks. Three excavators were each tasked with excavating two test pits: one using ALS, one in daylight conditions. The results of the experiment identified some critical aspects of using blue 455nm wavelength ALS in the field, and the importance of experienced practitioners. Sample evidence was small in size and recovery rates were low. In daylight conditions, an average of 46% of trace evidence was identified, while just 40% was recovered using ALS. This excludes hair fibres which were almost undetectable in all conditions. When using ALS, smaller bone fragments were more than twice as likely to be recovered, but less non-fluorescent materials were found. The experience of each excavator had a positive correlation with excavation results. Excavation error rates were calculated, demonstrating that excavation is comparable using either technique, but daylight conditions lead to greater accuracy. The findings suggest that ALS can be used to increase recovery of some evidence types. Test pits provided none of the usual primary evidence associated with graves and excavators had no prior experience of ALS. While retrieval rates were low, almost all recovered items were found in situ and an accurate records maintained. Error rates in forensic archaeology are essential and it is hoped that the method outlined here can be developed towards the establishment of acceptable error rates. While ALS use in forensic archaeology should not be considered a panacea to issues of trace evidence recovery, a combination of well-tested archaeological excavation methods, alongside the implementation of such proven forensic techniques, would likely lead to improved recovery of evidence., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The first author privately funded this work., (Copyright © 2020 Elsevier B.V. All rights reserved.)

This paper draws parallels between the current COVID-19 crisis and the apparent forensic science crisis. It investigates if shared approaches of the problem and solutions could emerge. Some relevant aspects of the medical system as it reacted to the pandemic crisis and the situation in forensic science are presented. Further, three main stages of the COVID-19 crisis and its impact on individuals and society are proposed, highlighting similarities to the effects of forensic science. Finally, some lessons from COVID-19 for forensic science are identified and discussed. It is concluded that forensic science's best assurance to address current and future challenges, particularly in an increasingly digital environment, remains a sound scientific approach, including critical thinking and inter-disciplinary collaborations., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

In burglary cases, the skeleton keys used by burglars often leave tool marks that can not be identified. Hence, forensic trace experts can only provide investigating authorities with useful information by categorizing the lock opening method of pin tumbler lock cylinders and defining the class characteristics of the tool used. It is important to note that as per the standard processes, expensive and more time consuming DNA-tests may not be applied to this evidence type. During the past few years, more and more dimple locks were documented by the police being removed from security doors at burglary scenes and sent to the Hungarian Institute for Forensic Sciences. These dimple locks were made in China with no brand mark, with 6-8 pin tumblers. We noticed previously unknown tool marks on the horizontal keyway, on the face and on the side of the tumblers of most of these dimple locks. We also found fragments of aluminium foil in the bottom of the plugs. Based on this, we came to the conclusion that the burglars work with a new method when opening this type of locks, which required forensic trace experts to introduce a new way of examination. In this study specific data is meant to be presented supporting the observations above. This paper is presenting a new lock opening method and the tool marks left behind. Our purpose was to develop a new method which takes the benefit of a new type of evidence: foil fragments noticed in the cylinder with possible DNA evidence, which was previously unknown by the police. This goes beyond the scope of general investigative findings, allowing a more effective identification of the burglars. We are also going to explicate the results of our DNA tests. Foil fragments having been recorded under sterile conditions brought searchable DNA-profiles in 52 % of the cases. In 55 % of these cases DNA-profiles were matched with suspects' and a series of crimes and groups of criminals were explored. Examining the DNA-profiles that did not match suspects' profiles revealed the presence of victims' DNA in certain cases. The outcomes of our study highlight that collecting victim DNA-samples should be implemented as a default process in cases where a DNA-test is required., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

A previous paper published in this journal proposed a model for evaluating the location of fingermarks on two-dimensional items (de Ronde, van Aken, de Puit and de Poot (2019)). In this paper, we apply the proposed model to a dataset consisting of letters to test whether the activity of writing a letter can be distinguished from the alternative activity of reading a letter based on the location of the fingermarks on the letters. An experiment was conducted in which participants were asked to read a letter and write a letter as separate activities on A4- and A5-sized papers. The fingermarks on the letters were visualized, and the resulting images were transformed into grid representations. A binary classification model was used to classify the letters into the activities of reading and writing based on the location of the fingermarks in the grid representations. Furthermore, the limitations of the model were studied by testing the influence of the length of the letter, the right- or left-handedness of the donor and the size of the paper with an additional activity of folding the paper. The results show that the model can predict the activities of reading or writing a letter based on the fingermark locations on A4-sized letters of right-handed donors with 98 % accuracy. Additionally, the length of the written letter and the handedness of the donor did not influence the performance of the classification model. Changing the size of the letters and adding an activity of folding the paper after writing on it decreased the model's accuracy. Expanding the training set with part of this new set had a positive influence on the model's accuracy. The results demonstrate that the model proposed by de Ronde, van Aken, de Puit and de Poot (2019) can indeed be applied to other two-dimensional items on which the disputed activities would be expected to lead to different fingermark locations. Moreover, we show that the location of fingermarks on letters provides valuable information about the activity that is carried out., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

Physical fit examinations have long played a critical role in forensic science, particularly in the trace evidence, toolmark, and questioned documents disciplines. Specifically, in trace evidence, physical fits arise in various instances such as separated pieces of duct tape, torn textile fragments, and fractured polymeric items to name a few. The case report and research basis for forensic physical fit dates to the late 1700s and varies by material type. Three main areas of physical fit appear within the literature: case reports, fractography studies, and quantitative assessment of a fracture fit. A strong foundation within the discipline lies in case reports, articles demonstrating occurrences of physical fit the authors have experienced in their laboratories. Fractography research offers information about the fracturing mechanism of a given material for purposes of identifying a potential breaking source. Also, fractography studies demonstrate variation in fracture morphology per material types, with a qualitative basis for comparison and reporting. The current shift in the research appears to be more quantitative or performance-based, assessing the error rates associated with physical fit examinations, the application of likelihood ratios as a means to determine evidential weight, probabilistic interpretations of large sample sets, and the implementation of automatic edge-detection algorithms to support the examiner's expert opinion. This review aims to establish the current state of physical fit research through what has been accomplished, the limitations faced due to the unpredictable nature of casework, and the future directions of the discipline. In addition, current practice in the field is evaluated through a review of standard operating procedures., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier B.V. All rights reserved.)