1. Role of adenosine on intestinal ischemia-reperfusion injury in rabbits.
- Author
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Taha MO, Miranda-Ferreira R, Simões RS, Abrão MS, Oliveira-Junior IS, Monteiro HP, Santos JM, Rodrigues PH, Rodrigues JV, Alves AE, Nascimento EC, Silva TL, Zeviani WM, and Caricati-Neto A
- Subjects
- Animals, Blood Circulation, Electric Stimulation, Femoral Vein drug effects, Femoral Vein physiology, Jejunum blood supply, Jejunum drug effects, Jejunum physiology, Male, Mesenteric Artery, Superior physiology, Muscle Contraction drug effects, Muscle Contraction physiology, Potassium Chloride pharmacology, Purinergic P1 Receptor Agonists, Rabbits, Sodium Chloride pharmacology, Adenosine pharmacology, Intestines blood supply, Reperfusion Injury drug therapy
- Abstract
To study if the treatment with adenosine (ADO), an agonist of adenosine receptors, attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), we treated rabbits with ADO (15 mg x kg(-1), intravenously) or saline solution (SS) to I (60 minutes) before occlusion of superior mesenteric artery and/or R (120 min). After I or I/R, isolated jejunal segments (2 cm) were mounted in an organ bath to study nerve-mediated contractions stimulated by electrical pulses or KCl using a digital recording system. Thin jejunal slices were stained (hematoxylin and eosin) for analysis by optical microscopy. Compared to the sham group, the jejunal contractions were similar in I + ADO, but reduced in I + SS, I/R + SS, and I/R + ADO groups. We concluded that the jejunal enteric nerves were damaged in I + SS, I/R + SS, and I/R + ADO, but not in I + ADO group. These results suggested that ADO attenuated intestinal dysfunction due to I, but not to R., (Copyright (c) 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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