1. Saponin loaded montmorillonite-human serum albumin nanocomposites as drug delivery system in colorectal cancer therapy
- Author
-
Betül Bozdoğan, Öznur Akbal, Soheil Malekghasemi, Tayfun Vural, Emir Baki Denkbaş, Fen-Edebiyat Fakültesi, BOZDOGAN, BETUL -- 0000-0003-1546-3895, and [Akbal, Oznur] Hacettepe Univ, Adv Technol Applicat & Res Ctr, TR-06800 Ankara, Turkey -- [Akbal, Oznur -- Denkbas, Emir Baki] Hacettepe Univ, Grad Sch Sci & Engn, Nanotechnol & Nanomed Div, TR-06800 Ankara, Turkey -- [Vural, Tayfun -- Denkbas, Emir Baki] Hacettepe Univ, Chem Dept, Biyochem Div, TR-06800 Ankara, Turkey -- [Malekghasemi, Soheil] Hacettepe Univ, Grad Sch Sci & Engn, Dept Bioengn, TR-06800 Ankara, Turkey -- [Bozdogan, Betul] Aksaray Univ, Fac Arts & Sci, Dept Chem, Biochem Div, TR-68100 Aksaray, Turkey
- Subjects
Colorectal cancer ,Saponin ,Serum albumin ,Cancer Treatment ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,behavioral disciplines and activities ,01 natural sciences ,DLD-1 cell ,Nanocomposites ,chemistry.chemical_compound ,Geochemistry and Petrology ,mental disorders ,medicine ,Cytotoxic T cell ,Montmorillonite ,Human Serum Albumin ,biology ,Geology ,021001 nanoscience & nanotechnology ,medicine.disease ,Human serum albumin ,0104 chemical sciences ,chemistry ,Toxicity ,Cancer cell ,Drug delivery ,biology.protein ,Glutaraldehyde ,0210 nano-technology ,medicine.drug - Abstract
WOS: 000449135300025, This study reports the synthesis and application of new saponin (SAP)-loaded montmorillonite-human serum albumin (Mt-HSA) nanocomposites (NCs) as an anticancer drug delivery agent. When the biodegradable and biofriendly HSA was combined with Mt., which possesses excellent mucoadhesive properties, the resulting NCs could pass through the gastrointestinal barrier easily. SAP-Mt-HSA NCs were prepared by a modified desolvation technique in which ethanol and glutaraldehyde (GA) were used as precipitating and crosslinking agents, respectively. The efficacy of these NCs was assessed in a colorectal cancer cell line (DLD-1) as an in-vitro gastrointestinal model and L929 fibroblast cells as a healthy cell model. The release profile of SAP and the cytotoxic effect and cellular uptake of NCs were evaluated. The results clearly demonstrated that SAP-Mt-HSA NCs can induce dose-dependent cancer cell death with little or no toxicity toward healthy cells.
- Published
- 2018