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In the field of local application of antimicrobials, a number of novel drugs and/or new drug delivery systems have been developed in recent years. The present study aimed to investigate hydroxyapatite cement (HAC) as a carrier for vancomycin in the treatment of chronic osteomyelitis due to Staphylococcus aureus strains with various mechanisms of resistance. The release of vancomycin from standard test cylinders was determined in vitro and the efficacy of the delivery system was measured in vivo using a rabbit model of chronic osteomyelitis. First, powdered HAC was mixed with vancomycin at 80, 160 and 240 mg/g. After hardening, formed cylinders were eluted in phosphate buffer and antibiotic release was measured by agar diffusion. High levels of release (1512+/-318 to 1937+/-336 microg/ml) were obtained for 12 to 20 days depending on the dosage of vancomycin. Additionally, bone infection was induced in the tibia of 30 New Zealand white rabbits by injecting either a methicillin-resistant S. aureus strain (MRSA) or a S. aureus strain with a small colony variant (SCV) phenotype. After 3 weeks (chronic infection), all animals were treated by debridement. Moreover, group 1 (challenged with SCVs) and group 2 (challenged with MRSA) were treated by filling the marrow with HAC alone, whereas in groups 3 (SCVs) and 4 (MRSA) the marrow was filled with HAC/vancomycin (160 mg/g). After 6 weeks all animals were sacrificed. At 3 weeks, pathogens were detected in 24 of 30 animals. All swabs of the control groups, positive for S. aureus on day 21, were also positive on day 42 and S. aureus strains recovered were shown to be clonal to the strains used for induction of osteomyelitis. By contrast, no growth was found in the treatment group following 7 days of incubation in BHI bouillon. HAC/vancomycin-treated animals showed no histological evidence of infection on day 42. In the other groups, different stages of chronic osteomyelitis were found histologically. No local or systemic side effects due to HAC or vancomycin were seen. HAC is an effective carrier material for antibiotic compounds even in refractory infections due to MRSA or S. aureus SCVs.

Objective: To determine the accuracy of rasterstereographic three-dimensional back surface analysis and reconstruction of the spine in idiopathic scoliosis treated by posterior correction and fusion., Design: Prospective imaging study of 25 patients with idiopathic scoliosis who underwent posterior correction and fusion and were followed for one year., Background: In an earlier study published in this journal rasterstereography has proved to be an accurate imaging modality for quantifying the changes in the three-dimensional shape of the spine and posterior rib cage after anterior correction and fusion. Goal of the present study was to determine the accuracy for the more common posterior correction and fusion with attention paid to the presence of the posterior implants and scarring., Methods: Twenty-five patients with idiopathic scoliosis with maximum Cobb angles of 78 degrees were examined by rasterstereography and radiography. Seventy-one anterior-posterior radiographs were digitised. Twenty-four were preoperative and 47 postoperative radiographs. Rasterstereographic and radiographic curves were compared by best-fit superimposition. Root-mean-square differences were calculated as parameters of accuracy., Results: The accuracy of rasterstereography in severe idiopathic scoliosis with Cobb angles between 48 degrees and 78 degrees was satisfactory with root-mean-square differences of 5.8 mm for the lateral deviation and 4.8 degrees for vertebral rotation. Following posterior correction the accuracy was good. The root-mean-square difference was 4.5 mm for the lateral deviation and 4.3 degrees for vertebral rotation., Conclusion: The accuracy obtained for posteriorly-operated scolioses between 50 degrees and 80 degrees was similar to the findings for scolioses operated via anterior approach, as well as those with curves up to 50 degrees Cobb angle. Therefore rasterstereography can be used postoperatively to reduce the number of radiographs and radiation exposure. Additionally, the method provides an objective quantification of the postoperative improvement in the cosmesis of the back shape., Relevance: In the treatment of severe idiopathic scoliosis rasterstereography provides both a considerable reduction of X-rays and an objective documentation of the cosmesis before after scoliosis surgery.

Objective: To determine the accuracy of rasterstereographic three-dimensional back surface analysis and reconstruction of the spine in cases of severe idiopathic scoliosis treated by anterior correction and fusion., Design: Comparison of digitized radiographic curves and rasterstereographic curves by best fit superimposition and calculation of root mean square differences as parameters of similarity., Background: Rasterstereography has been proven to be accurate in scoliosis up to 50 degrees Cobb angle. Since 1989 the device is in clinical routine use for non-operatively treated patients and reduces the need for otherwise indispensable radiographs significantly., Methods: Fifty two patients with severe idiopathic scoliosis with Cobb angles up to 88 degrees were examined rasterstereographically and radiographically. Forty eight pre-operative anterior-posterior radiographs and 101 post-operative anterior-posterior radiographs were digitized. Radiographic and rasterstereographic curves were compared and the root mean square differences were calculated as parameters of precision of rasterstereographic reconstruction., Results: Accuracy of rasterstereography in idiopathic scoliosis with Cobb angles between 50 degrees and 88 degrees is satisfactory. The root mean square difference of the radiographic and rasterstereographic curves was 6.4 mm for lateral deviation and 4.5 degrees for vertebral rotation. After anterior scoliosis surgery the precision of the device is good. The root mean square difference for lateral deviation was 3.4 mm and 3.2 degrees for rotation. Considering both groups an average root mean square of 4.7 mm and 3.7 degrees was calculated., Conclusions: Accuracy in severe scoliosis up to 88 degrees Cobb angle was satisfactory. The results of this first evaluation of surgically treated severe scoliosis showed a good accuracy after anterior surgery. The system can be used for post-operative follow up examinations and may reduce the number of X-rays considerably. In contrast to radiography, CT or MRI rasterstereography provides an objective quantification and documentation of the post-operative cosmetic improvement of the back shape in standing posture. RELEVANCE STATEMENT: Based on the findings of this study rasterstereography in future enables both objective quantification of cosmetic improvement and significant reduction of X-rays in idiopathic scoliosis with Cobb angles higher than 50 degrees before and after anterior surgical correction and fusion.