1. Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags
- Author
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Volkmar Gieselmann, Nahal Brocke-Ahmadinejad, Frederic Brosseron, Marc Sylvester, Teodora Stella Wijasa, Markus P. Kummer, and Michael T. Heneka
- Subjects
0301 basic medicine ,Proteomics ,Male ,Proteome ,Analytical chemistry ,Short Communications ,metabolism [Cerebrum] ,metabolism [White Matter] ,metabolism [Gray Matter] ,Nerve Tissue Proteins ,Computational biology ,Mass spectrometry ,Mass Spectrometry ,03 medical and health sciences ,Mice ,Animals ,Humans ,ddc:610 ,Gray Matter ,metabolism [Synaptosomes] ,Cerebrum ,ComputingMethodologies_COMPUTERGRAPHICS ,metabolism [Nerve Tissue Proteins] ,Chemistry ,General Neuroscience ,Chromatography liquid ,White Matter ,030104 developmental biology ,Proteome profiling ,Isobaric process ,methods [Proteomics] ,Synaptosomes ,Chromatography, Liquid - Abstract
Graphical abstract, Highlights • Protocol for quantitative proteomics of nitrosylation on synaptosomal proteins. • Identification of endogenous nitrosylation independent of induction by NO donors. • Use of iodoTMT sixplex mass tags for stable labeling, enrichment, identification, and multiplex quantitation. • Applicable on low amounts of sample material of mouse and human brain tissue.
- Published
- 2017
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