1. An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors.
- Author
-
Cruz DD, Nava-García E, and Arellano E
- Subjects
- Animals, Humans, Polymerase Chain Reaction, Chagas Disease parasitology, Chagas Disease transmission, Chemical Fractionation methods, DNA, Protozoan isolation & purification, Disease Vectors, Trypanosoma cruzi genetics
- Abstract
An improved protocol for DNA extraction for the Chagas disease vectors is proposed based on modification to a low cost method described twenty years ago. Quality DNA and high molecular weight were obtained from all samples. NADH-4 gene was successfully amplified by PCR using the isolated DNA. The extraction protocol presented in this technical note is a fast, low-cost, and non-aggressive method to human health for obtaining genetic data from this group of epidemiological importance and potentially other insects., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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