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A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detection.

A rapid and specific high-performance liquid chromatographic assay for the quantitative determination of angiotensin-converting enzyme activity is described. Hippuryl-L-histidyl-L-leucine (Hip-His-Leu) is used as substrate and the released hippuric acid is measured. The procedure is accurate and precise and no extraction is required.

A simple, specific, and sensitive high-performance liquid chromatographic method for the determination of papaveraldine and papaverinol in papaverine hydrochloride injection has been developed. The compounds were chromatographed on a reversed-phase C-18 column using a water-methanol-acetonitrile solvent system containing sodium lauryl sulphate ion-pair reagent.

A reversed-phase high-performance liquid chromatographic method has been developed to determine paracetamol, caffeine and propyphenazone in a typical tablet formulation with high accuracy and precision. The mobile phase is a linear water-methanol gradient.