1. A diphenyl diselenide-supplemented diet and swimming exercise promote neuroprotection, reduced cell apoptosis and glial cell activation in the hypothalamus of old rats.
- Author
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Leite MR, Cechella JL, Pinton S, Nogueira CW, and Zeni G
- Subjects
- Animals, Antigens, Nuclear metabolism, Astrocytes drug effects, Biomarkers metabolism, Brain-Derived Neurotrophic Factor metabolism, Dietary Supplements, Glial Fibrillary Acidic Protein metabolism, Homeostasis, Hypothalamus metabolism, Male, Microglia drug effects, Nerve Tissue Proteins metabolism, Physical Conditioning, Animal, Poly(ADP-ribose) Polymerases metabolism, Rats, Rats, Wistar, Aging physiology, Apoptosis physiology, Benzene Derivatives pharmacology, Hypothalamus drug effects, Neuroprotection, Organoselenium Compounds pharmacology, Swimming physiology
- Abstract
Aging is a process characterized by deterioration of the homeostasis of various physiological systems; although being a process under influence of multiple factors, the mechanisms involved in aging are not well understood. Here we investigated the effect of a (PhSe)2-supplemented diet (1ppm, 4weeks) and swimming exercise (1% of body weight, 20min per day, 4weeks) on proteins related to glial cells activation, apoptosis and neuroprotection in the hypothalamus of old male Wistar rats (27month-old). Old rats had activation of astrocytes and microglia which was demonstrated by the increase in the levels of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule 1 (Iba-1) in hypothalamus. A decrease of B-cell lymphoma 2 (Bcl-2) and procaspase-3 levels as well as an increase of the cleaved PARP/full length PARP ratio (poly (ADP-ribose) polymerase, PARP) and the pJNK/JNK ratio (c-Jun N-terminal kinase, JNK) were observed. The levels of mature brain-derived neurotrophic factor (mBDNF), the pAkt/Akt ratio (also known as protein kinase B) and NeuN (neuronal nuclei), a neuron marker, were decreased in the hypothalamus of old rats. Old rats that received a (PhSe)2-supplemented diet and performed swimming exercise had the hypothalamic levels of Iba-1 and GFAP decreased. The combined treatment also increased the levels of Bcl-2 and procaspase-3 and decreased the ratios of cleaved PARP/full length PARP and pJNK/JNK in old rats. The levels of mBDNF and NeuN, but not the pAkt/Akt ratio, were increased by combined treatment. In conclusion, a (PhSe)2-supplemented diet and swimming exercise promoted neuroprotection in the hypothalamus of old rats, reducing apoptosis and glial cell activation., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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