1. Superior protection against paratuberculosis by a heterologous prime-boost immunization in a murine model.
- Author
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Colombatti Olivieri MA, Cuerda MX, Moyano RD, Gravisaco MJ, Pinedo MFA, Delgado FO, Calamante G, Mundo S, de la Paz Santangelo M, Romano MI, Alonso MN, and Del Medico Zajac MP
- Subjects
- Animals, Mice, Female, Bacterial Vaccines immunology, Bacterial Vaccines administration & dosage, Mice, Inbred BALB C, Vaccinia virus immunology, Vaccinia virus genetics, Antigens, Bacterial immunology, Antigens, Bacterial genetics, Immunity, Cellular immunology, Vaccines, Synthetic immunology, Vaccines, Synthetic administration & dosage, Freund's Adjuvant administration & dosage, Freund's Adjuvant immunology, Mycobacterium avium subsp. paratuberculosis immunology, Immunization, Secondary methods, Paratuberculosis prevention & control, Paratuberculosis immunology, Immunoglobulin G blood, Cytokines metabolism, Antibodies, Bacterial blood, Antibodies, Bacterial immunology, Adjuvants, Immunologic administration & dosage, Disease Models, Animal
- Abstract
Vaccination is the best strategy to control Paratuberculosis (PTB), which is a significant disease in cattle and sheep. Previously we showed the humoral and cellular immune response induced by a novel vaccine candidate against PTB based on the Argentinian Mycobacterium avium subspecies paratuberculosis (Map) 6611 strain. To improve 6611 immunogenicity and efficacy, we evaluated this vaccine candidate in mice with two different adjuvants and a heterologous boost with a recombinant modified vaccinia Ankara virus (MVA) expressing the antigen 85A (MVA85A). We observed that boosting with MVA85A did not improve total IgG or specific isotypes in serum induced by one or two doses of 6611 formulated with incomplete Freund's adjuvant (IFA). However, when 6611 was formulated with ISA201 adjuvant, MVA85A boost enhanced the production of IFNγ, Th1/Th17 cytokines (IL-2, TNF, IL-17A) and IL-6, IL-4 and IL-10. Also, this group showed the highest levels of IgG2b and IgG3 isotypes, both important for better protection against Map infection in the murine model. Finally, the heterologous scheme elicited the highest levels of protection after Map challenge (lowest CFU count and liver lesion score). In conclusion, our results encourage further evaluation of 6611 strain + ISA201 prime and MVA85A boost in bovines., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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