1. The NS-1 polypeptide of the autonomous parvovirus MVM is a nuclear phosphoprotein.
- Author
-
Cotmore SF and Tattersall P
- Subjects
- Cell Line, DNA, Viral analysis, Electrophoresis, Polyacrylamide Gel, Parvoviridae genetics, Peptide Biosynthesis, Peptides genetics, Phosphoproteins genetics, Phosphorylation, Protein Biosynthesis, RNA, Messenger metabolism, RNA, Viral metabolism, Viral Proteins genetics, Cell Nucleus analysis, Parvoviridae metabolism, Phosphoproteins biosynthesis, Viral Proteins biosynthesis
- Abstract
Cells infected with the autonomous parvovirus MVM synthesize a major virally coded non-structural protein which accumulates in the nucleus and is phosphorylated to a significant extent. Peptide map analysis shows that this in vivo product has the same primary sequence as the NS-1 protein previously identified in the in vitro translation products of MVM encoded mRNA, and as such is the product of the largest messenger RNA species, a spliced 4.8 kb (R1) transcript. In vivo NS-1 exists in two predominant forms, a phosphorylated 84-85 kDa species and an unphosphorylated or poorly phosphorylated 83 kDa form. Both forms are preferentially extracted from the nucleus using an extraction procedure which enriches for replication complexes.
- Published
- 1986
- Full Text
- View/download PDF