1. The effect of thermal acclimation on the activity of arylhydrocarbon hydroxylase in rainbow trout (Oncorhynchus mykiss).
- Author
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Carpenter HM, Fredrickson LS, Williams DE, Buhler DR, and Curtis LR
- Subjects
- Animals, Aryl Hydrocarbon Hydroxylases isolation & purification, Blotting, Western, Cytochrome P-450 Enzyme System isolation & purification, Isoenzymes isolation & purification, Kinetics, Temperature, Acclimatization, Aryl Hydrocarbon Hydroxylases metabolism, Cytochrome P-450 Enzyme System metabolism, Isoenzymes metabolism, Microsomes, Liver enzymology, Trout physiology
- Abstract
1. The possibility that temperature acclimation (to 10 or 18 degrees C for 28 days) would alter the cytochromes P-450 of rainbow trout was addressed. 2. The specific content of LM4b (P-450 IA1), the trout isozyme responsible for activation of polynuclear aromatic hydrocarbons, was lower in 18 degrees C fish than it was in 10 degrees C fish. 3. Kinetic analysis of aryl hydrocarbon hydroxylase indicated that, while thermal acclimation caused no change in Vmax, it lowered the apparent Km of this enzyme for benzo[a]pyrene when assayed at acutely shifted temperatures. 4. Thermal acclimation of fish may have significance when feral populations are subjected to acute temperature shifts.
- Published
- 1990
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