1. Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper.
- Author
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Blessborn D, Römsing S, Annerberg A, Sundquist D, Björkman A, Lindegardh N, and Bergqvist Y
- Subjects
- Humans, Lumefantrine, Paper, Reproducibility of Results, Sensitivity and Specificity, Solid Phase Extraction, Antimalarials blood, Antimalarials isolation & purification, Chromatography, Liquid methods, Ethanolamines blood, Ethanolamines isolation & purification, Fluorenes blood, Fluorenes isolation & purification
- Abstract
A bioanalytical method for the determination of lumefantrine in 100 microl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0.75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-day variation was below 10% over the range 0.4-25 microM. The lower limit of quantification was 0.25 microM in 100 microl capillary blood. No decrease in lumefantrine concentration in dried blood spot is seen after 4 months storage at 22 degrees C. The method was also evaluated in field samples from patients in Tanzania after treatment with lumefantrine/artemether. Lumefantrine could be estimated accurately enough to assess bioavailability and treatment compliance on day 7 (i.e. 4 days after the last dose) after a standard regimen with the lumefantrine/artemether combination.
- Published
- 2007
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