1. Effective protection of biological membranes against photo-oxidative damage: Polymeric antioxidant forming a protecting shield over the membrane.
- Author
-
Mertins O, Mathews PD, Gomide AB, Baptista MS, and Itri R
- Subjects
- Antioxidants radiation effects, Cell Membrane chemistry, Cell Membrane radiation effects, Chitosan radiation effects, Coated Materials, Biocompatible radiation effects, Light, Materials Testing, Oxidation-Reduction radiation effects, Oxygen chemistry, Radiation-Protective Agents radiation effects, Unilamellar Liposomes radiation effects, Antioxidants chemical synthesis, Chitosan chemistry, Coated Materials, Biocompatible chemical synthesis, Gallic Acid chemistry, Radiation-Protective Agents chemistry, Unilamellar Liposomes chemistry
- Abstract
We have prepared a chitosan polymer modified with gallic acid in order to develop an efficient protection strategy biological membranes against photodamage. Lipid bilayers were challenged with photoinduced damage by photosensitization with methylene blue, which usually causes formation of hydroperoxides, increasing area per lipid, and afterwards allowing leakage of internal materials. The damage was delayed by a solution of gallic acid in a concentration dependent manner, but further suppressed by the polymer at very low concentrations. The membrane of giant unilamellar vesicles was covered with this modified macromolecule leading to a powerful shield against singlet oxygen and thus effectively protecting the lipid membrane from oxidative stress. The results have proven the discovery of a promising strategy for photo protection of biological membranes., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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