1. Lipid droplet levels vary heterogeneously in response to simulated gastrointestinal stresses in different probiotic Saccharomyces cerevisiae strains
- Author
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Marcos Aronovich, Bruno Douradinha, Fernando A. Bozza, Mónica Montero-Lomelí, Daniel Zamith-Miranda, Flaviano S. Martins, Leonardo Nimrichter, Patrícia T. Bozza, Clarissa M. Maya-Monteiro, Gabriel S. Matos, Ernesto T. A. Marques, Mariana L. Palma, and Johnathon G. Schiebel
- Subjects
0301 basic medicine ,Saccharomyces cerevisiae var boulardii ,Therapeutic action ,Saccharomyces cerevisiae ,Medicine (miscellaneous) ,Saccharomyces ,law.invention ,Microbiology ,03 medical and health sciences ,Probiotic ,law ,Lipid droplet ,TX341-641 ,Gastrointestinal tract stresses ,Nutrition and Dietetics ,biology ,Nutrition. Foods and food supply ,Probiotics ,technology, industry, and agriculture ,biology.organism_classification ,Stress resistance ,Lipid droplets ,030104 developmental biology ,Induced stress ,Food Science - Abstract
To exert their therapeutic action, probiotic Saccharomyces cerevisiae strains must survive harsh digestive environments. Lipid droplets accumulate in cells which undergo stress-inducing situations, supposedly having a protective role. We assessed lipid droplet levels, either naturally accumulated or induced in response to digestive challenges, of probiotic strains S. boulardii, S. cerevisiae A-905, S. cerevisiae Sc47 and S. cerevisiae L11, and of non-probiotic strains S. cerevisiae BY4741 and S. cerevisiae BY4743. Strains 905 and Sc47 had lower and higher lipid droplet levels, respectively, when compared to the remaining strains, showing that higher accumulationof these neutral lipids is not a feature shared by all probiotic Saccharomyces strains. When submitted to simulated gastric or bile salts environments, lipid droplet levels increase in all tested probiotic strains, at least for one to the induced stresses, suggesting that lipid droplets participate in the protective mechanisms against gastrointestinal stresses in probiotic Saccharomyces yeasts.
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