1. Primary metabolism changes triggered in soybean leaves by Fusarium tucumaniae infection
- Author
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Alicia G. Luque, Gerardo Domingo Lucio Cervigni, Mónica E. Hourcade, Claudia P. Spampinato, María Mercedes Scandiani, Romina G. Rosati, and Luciana D. Lario
- Subjects
0106 biological sciences ,0301 basic medicine ,Fusarium ,Plant Science ,SUDDEN DEATH SYNDROME ,METABOLOMICS ,01 natural sciences ,Ciencias Biológicas ,03 medical and health sciences ,Genetics ,Metabolomics ,GC–MS ,Cultivar ,Pathogen ,Disease Resistance ,Peroxidase ,Plant Diseases ,Chlorosis ,biology ,Inoculation ,fungi ,RuBisCO ,food and beverages ,General Medicine ,Sudden infant death syndrome ,GLYCINE MAX ,Bioquímica y Biología Molecular ,biology.organism_classification ,PLANT SUSCEPTIBILITY ,Plant Leaves ,Horticulture ,030104 developmental biology ,Fusariosis ,biology.protein ,PATHOGEN INFECTION ,Electrophoresis, Polyacrylamide Gel ,Soybeans ,Agronomy and Crop Science ,CIENCIAS NATURALES Y EXACTAS ,010606 plant biology & botany - Abstract
Sudden death syndrome (SDS) of soybean can be caused by at least four distinct Fusarium species, with F. tucumaniae being the main causal agent in Argentina. The fungus is a soil-borne pathogen that is largely confined to the roots, but damage also reaches aerial part of the plant and interveinal chlorosis and necrosis, followed by premature defoliation can be observed. In this study, two genetically diverse soybean cultivars, one susceptible (NA 4613) and one partially resistant (DM 4670) to SDS infection, were inoculated with F. tucumaniae or kept uninoculated. Leaf samples at 7, 10, 14 and 25 days post-inoculation (dpi) were chosen for analysis. With the aim of detecting early markers that could potentially discriminate the cultivar response to SDS, gas chromatography–mass spectrometry (GC–MS) analyses and biochemical studies were performed. Metabolic analyses show higher levels of several amino acids in the inoculated than in the uninoculated susceptible cultivar starting at 10 dpi. Biochemical studies indicate that pigment contents and Rubisco level were reduced while class III peroxidase activity was increased in the inoculated susceptible plant at 10 dpi. Taken together, our results indicate that the pathogen induced an accumulation of amino acids, a decrease of the photosynthetic activity, and an increase of plant-specific peroxidase activity in the susceptible cultivar before differences of visible foliar symptoms between genotypes could be observed, thus suggesting that metabolic and biochemical approaches may contribute to a rapid characterization of the cultivar response to SDS. Fil: Rosati, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lario, Luciana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Hourcade, Mónica E.. Universidad Nacional de Rosario; Argentina Fil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Luque, Alicia G.. Universidad Nacional de Rosario; Argentina Fil: Scandiani, María Mercedes. Universidad Nacional de Rosario; Argentina Fil: Spampinato, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
- Published
- 2018