1. Epidemiology of human respiratory viruses in children with acute respiratory tract infection in a 3-year hospital-based survey in Northern Italy☆
- Author
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Flora De Conto, F. Ferraglia, Maria Cristina Medici, Maria Cristina Arcangeletti, Adriana Calderaro, Carlo Chezzi, F. Pinardi, and Francesca Conversano
- Subjects
0301 basic medicine ,Male ,Microbiological Techniques ,Epidemiology ,viruses ,BAL, bronchial alveolar lavage ,Acute respiratory tract infections ,0302 clinical medicine ,PCR, polymerase chain reaction ,HEP-2, human larynx epidermoid carcinoma ,Surveys and Questionnaires ,rt-RT-PCR, reverse transcription RT-PCR ,030212 general & internal medicine ,Respiratory system ,Child ,Acute respiratory tract infection ,HMPV, human metapneumovirus ,Respiratory Tract Infections ,Respiratory tract infections ,ARTI, acute respiratory tract infection ,Coinfection ,Incidence (epidemiology) ,Incidence ,HBOV, human bocavirus ,Molecular assays ,SP, sputum ,General Medicine ,Hospital based ,IBV, influenza virus B ,IAV, influenza virus A ,Hospitals ,Infectious Diseases ,CPE, cytopathic effect ,Italy ,HCOV, human coronavirus ,Virus Diseases ,Child, Preschool ,Viruses ,IV, influenza virus ,HEV, human enterovirus ,Female ,LLC-MK2, rhesus monkey-kidney ,RT-PCR, real-time PCR ,Microbiology (medical) ,medicine.medical_specialty ,Adolescent ,030106 microbiology ,PBS, phosphate-buffered saline ,MDCK-SIAT1, Madin-Darby Canine Kidney with enhanced expression of 6-linked sialic acids ,MRC-5, human lung fibroblasts ,Virus ,Article ,TS, throat swab ,03 medical and health sciences ,medicine ,NS, nasal swab ,Humans ,mAb, monoclonal antibody ,HADV, human adenovirus ,IIF, indirect immunofluorescence assay ,Respiratory viruses ,Virus isolation in cell culture ,business.industry ,Diagnostic Tests, Routine ,Vero, African green monkey kidney ,Intestine 407, embryonic human intestine ,Infant, Newborn ,ECHO, echovirus ,Infant ,HPIV, human parainfluenza virus ,Virology ,BAS, bronchial aspirate ,Northern italy ,HRSV, human respiratory syncytial virus ,NPA, nasopharyngeal aspirate ,Laboratory diagnosis ,BSA, bovine serum albumin ,business ,CV, coxsackievirus - Abstract
Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012–September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50.44%); 1037 (79.83%) were single infections and 262 (20.17%) mixed infections. The highest infection incidence was in children aged >6 months to ≤3 years (57.36%). Human respiratory syncytial virus (27.12%) and human adenovirus (23.58%) were the most common viruses identified. The virus detection rate decreased significantly between the first and third epidemic season (53.9% vs. 43.05%, P, Highlights • Respiratory viruses were assessed in children from October 2012 to September 2015. • Viruses were detected using antigen and molecular assays, and cell culture. • Respiratory syncytial virus and adenovirus were the most common viruses detected. • Influenza virus and respiratory syncytial virus detection showed seasonal variation. • Respiratory virus detection was highest in children aged >6 months to ≤3 years.
- Published
- 2019