1. Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
- Author
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Vasco Fachada, Paavo Rahkila, Nuno Fachada, Tuomas Turpeinen, Urho M. Kujala, and Heikki Kainulainen
- Subjects
Histology ,lipid droplets ,Muscle Fibers, Skeletal ,Skeletal muscle ,lihakset ,lipidit ,Perilipin-5 ,Type II diabetes ,SDG 3 - Good Health and Well-being ,insulin resistance ,Humans ,skeletal muscle ,Muscle, Skeletal ,Insulin resistance ,Cell Biology ,General Medicine ,Lipid Metabolism ,Lipid droplets ,insuliiniresistenssi ,fiber type ,PLIN5 ,Diabetes Mellitus, Type 2 ,proteiinit ,Fiber type ,aikuistyypin diabetes - Abstract
Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions. peerReviewed
- Published
- 2022
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