1. Liver damage and caspase-dependent apoptosis is related to protein malnutrition in mice: Effect of methionine
- Author
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Ruben Danilo Conde, Ana Marcela Giudici, José A. Ferragut, Veronica Jorgelina Caballero, Daniel Marcelo Lombardo, Miguel Saceda, and Julieta Renee Mendieta
- Subjects
medicine.medical_specialty ,Histology ,mice ,Protein Carbonylation ,Apoptosis ,X-Linked Inhibitor of Apoptosis Protein ,medicine.disease_cause ,liver ,Superoxide dismutase ,Ciencias Biológicas ,Mice ,chemistry.chemical_compound ,Methionine ,Protein Deficiency ,Internal medicine ,medicine ,Animals ,methionine ,Mice, Inbred BALB C ,biology ,protein depletion ,Caspase 3 ,apoptosis ,Apoptosis Inducing Factor ,Cell Biology ,General Medicine ,Bioquímica y Biología Molecular ,XIAP ,Fatty acid synthase ,Endocrinology ,Glutathione S-Transferase pi ,Biochemistry ,chemistry ,biology.protein ,Apoptosis-inducing factor ,Female ,Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) ,Oxidative stress ,CIENCIAS NATURALES Y EXACTAS - Abstract
This study aimed to determine whether the effects on the mouse liver caused by three periods of feeding a protein-free diet for 5 days followed by a normal complete diet for 5 days (3PFD-CD) are prevented by a constant methionine supply (3PFD + Met-CD). The expressions of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) were assessed by proteomics and reverse transcriptase-polymerase chain reactions. The liver redox status was examined by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein carbonylation. Because oxidative stress can result in apoptosis, the activity and content of caspase-3, as well as the x-linked inhibitor of the apoptosis protein (XIAP) and mitochondrial caspase-independent apoptosis inducing factor (AIF) contents were assessed. In addition, the liver histomorphology was examined. Compared to the controls fed a normal complete diet throughout, feeding with 3PFD-CD increased the FAS content, decreased the CAIII content, decreased both the SOD and CAT activities, and increased protein carbonylation. It also activated caspase-3, decreased the XIAP content, decreased the AIF content, increased the number of GSTP1-positive foci and caspase-3-positive cells, and caused fatty livers. Conversely, the changes were lessened to varying degrees in mice fed 3PFD + Met-CD. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver. Fil: Caballero, Veronica Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina Fil: Saceda, Miguel. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España Fil: Ferragut, José Antonio. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
- Published
- 2014