1. Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene-α (Gro-α) from endometrioma stromal cells.
- Author
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Takamura M, Osuga Y, Izumi G, Yoshino O, Koga K, Saito A, Hirata T, Hirota Y, Harada M, Hasegawa A, and Taketani Y
- Subjects
- Cells, Cultured, Chemotaxis, Leukocyte, Culture Media, Conditioned metabolism, Endometriosis immunology, Endometriosis pathology, Endometriosis surgery, Endometrium immunology, Endometrium pathology, Endometrium surgery, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Hospitals, University, Humans, Immunohistochemistry, Japan, Neutrophils immunology, Neutrophils pathology, Stromal Cells immunology, Stromal Cells pathology, Chemokine CXCL1 metabolism, Endometriosis metabolism, Endometrium metabolism, Interleukin-17 metabolism, Neutrophils metabolism, Paracrine Communication, Stromal Cells metabolism
- Abstract
Objective: To investigate a new role of interleukin (IL)-17A in endometriosis., Design: Laboratory study., Setting: University hospital., Patient(s): Patients with ovarian endometrioma undergoing laparoscopy or laparotomy., Intervention(s): Primary culture of endometrioma stromal cells (EoSCs) was stimulated with IL-17A. Sections of endometrioma tissue were immunostained with antibodies for IL-17A, growth-regulated oncogene-α (Gro-α), and elastase, a marker of neutrophils. They were also examined with immunofluorescent double staining for IL-17A and myeloperoxidase, another marker of neutrophils., Main Outcome Measure(s): Concentration of Gro-α was measured using a specific ELISA. Neutrophil chemotaxis was measured with Boyden chamber method. Immunostained sections were examined under microscope., Result(s): Interleukin-17A increased the secretion of Gro-α from EoSCs dose-dependently. The conditioned medium of EoSCs stimulated with IL-17A attracted more neutrophils than that of EoSCs stimulated with vehicle, and the increase was inhibited by the addition of Gro-α-neutralizing antibody. On immunostaining, IL-17A and Gro-α were detected in similar areas of the stroma beneath the epithelium, where Gro-α was detected in cells with a stromal cell appearance whereas IL-17A was detected in neutrophils as determined by detection of elastase. Fluorescent immunostaining corroborated that myeloperoxidase-positive neutrophils were also positive for IL-17A., Conclusion(s): It is suggested that IL-17A produced by neutrophils stimulates Gro-α secretion from EoSCs, thereby recruiting more neutrophils and inducing perpetuating inflammation in endometriosis., (Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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