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3. 3D printing novel porous granule-type bioceramics via magnesium tuning biological performances beneficial for implantation and clinical translation

Author

Yue, Xusong, primary, Jiao, Xiaoyi, additional, Xu, Chuchu, additional, Zhang, Yan, additional, Wu, Fanghui, additional, Wang, Haojie, additional, Zhu, Qiao, additional, Zhang, Zhan, additional, Zhao, Liben, additional, Sun, Xiaoliang, additional, Yang, Xianyan, additional, He, Fuming, additional, Gou, Zhongru, additional, Yang, Guojing, additional, and Zhang, Lei, additional

Published
2024
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6. The 2023 report of the Lancet Countdown on health and climate change: the imperative for a health-centred response in a world facing irreversible harms

Author

Romanello, Marina, primary, Napoli, Claudia di, additional, Green, Carole, additional, Kennard, Harry, additional, Lampard, Pete, additional, Scamman, Daniel, additional, Walawender, Maria, additional, Ali, Zakari, additional, Ameli, Nadia, additional, Ayeb-Karlsson, Sonja, additional, Beggs, Paul J, additional, Belesova, Kristine, additional, Berrang Ford, Lea, additional, Bowen, Kathryn, additional, Cai, Wenjia, additional, Callaghan, Max, additional, Campbell-Lendrum, Diarmid, additional, Chambers, Jonathan, additional, Cross, Troy J, additional, van Daalen, Kim R, additional, Dalin, Carole, additional, Dasandi, Niheer, additional, Dasgupta, Shouro, additional, Davies, Michael, additional, Dominguez-Salas, Paula, additional, Dubrow, Robert, additional, Ebi, Kristie L, additional, Eckelman, Matthew, additional, Ekins, Paul, additional, Freyberg, Chris, additional, Gasparyan, Olga, additional, Gordon-Strachan, Georgiana, additional, Graham, Hilary, additional, Gunther, Samuel H, additional, Hamilton, Ian, additional, Hang, Yun, additional, Hänninen, Risto, additional, Hartinger, Stella, additional, He, Kehan, additional, Heidecke, Julian, additional, Hess, Jeremy J, additional, Hsu, Shih-Che, additional, Jamart, Louis, additional, Jankin, Slava, additional, Jay, Ollie, additional, Kelman, Ilan, additional, Kiesewetter, Gregor, additional, Kinney, Patrick, additional, Kniveton, Dominic, additional, Kouznetsov, Rostislav, additional, Larosa, Francesca, additional, Lee, Jason K W, additional, Lemke, Bruno, additional, Liu, Yang, additional, Liu, Zhao, additional, Lott, Melissa, additional, Lotto Batista, Martín, additional, Lowe, Rachel, additional, Odhiambo Sewe, Maquins, additional, Martinez-Urtaza, Jaime, additional, Maslin, Mark, additional, McAllister, Lucy, additional, McMichael, Celia, additional, Mi, Zhifu, additional, Milner, James, additional, Minor, Kelton, additional, Minx, Jan C, additional, Mohajeri, Nahid, additional, Momen, Natalie C, additional, Moradi-Lakeh, Maziar, additional, Morrissey, Karyn, additional, Munzert, Simon, additional, Murray, Kris A, additional, Neville, Tara, additional, Nilsson, Maria, additional, Obradovich, Nick, additional, O'Hare, Megan B, additional, Oliveira, Camile, additional, Oreszczyn, Tadj, additional, Otto, Matthias, additional, Owfi, Fereidoon, additional, Pearman, Olivia, additional, Pega, Frank, additional, Pershing, Andrew, additional, Rabbaniha, Mahnaz, additional, Rickman, Jamie, additional, Robinson, Elizabeth J Z, additional, Rocklöv, Joacim, additional, Salas, Renee N, additional, Semenza, Jan C, additional, Sherman, Jodi D, additional, Shumake-Guillemot, Joy, additional, Silbert, Grant, additional, Sofiev, Mikhail, additional, Springmann, Marco, additional, Stowell, Jennifer D, additional, Tabatabaei, Meisam, additional, Taylor, Jonathon, additional, Thompson, Ross, additional, Tonne, Cathryn, additional, Treskova, Marina, additional, Trinanes, Joaquin A, additional, Wagner, Fabian, additional, Warnecke, Laura, additional, Whitcombe, Hannah, additional, Winning, Matthew, additional, Wyns, Arthur, additional, Yglesias-González, Marisol, additional, Zhang, Shihui, additional, Zhang, Ying, additional, Zhu, Qiao, additional, Gong, Peng, additional, Montgomery, Hugh, additional, and Costello, Anthony, additional

Published
2023
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14. SCYL3, as a novel binding partner and regulator of ROCK2, promotes hepatocellular carcinoma progression

Author

Lei, Martina Mang Leng, primary, Leung, Carmen Oi Ning, additional, Lau, Eunice Yuen Ting, additional, Leung, Rainbow Wing Hei, additional, Ma, Victor Wan San, additional, Tong, Man, additional, Lu, Yin Ying, additional, Huang, Chen Yang, additional, Zhu, Qiao Hua, additional, Ng, Irene Oi Lin, additional, Ma, Stephanie, additional, and Lee, Terence Kin Wah, additional

Published
2023
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22. Cryo-EM structure of the entire FtsH-HflKC AAA protease complex

Author

Zhu Qiao, Tatsuhiko Yokoyama, Xin-Fu Yan, Ing Tsyr Beh, Jian Shi, Sandip Basak, Yoshinori Akiyama, Yong-Gui Gao, School of Biological Sciences, and NTU Institute of Structural Biology

Subjects
Escherichia coli Proteins, AAA protease, Cryoelectron Microscopy, Biological sciences [Science], HflKC, prohibitin, General Biochemistry, Genetics and Molecular Biology, AAA Protease, SPFH, Protein Quality Control, ATP-Dependent Proteases, Bacterial Proteins, Escherichia coli, ATPases Associated with Diverse Cellular Activities, Humans, protein quality control, FtsH
Abstract

The membrane-bound AAA protease FtsH is the key player controlling protein quality in bacteria. Two single-pass membrane proteins, HflK and HflC, interact with FtsH to modulate its proteolytic activity. Here, we present structure of the entire FtsH-HflKC complex, comprising 12 copies of both HflK and HflC, all of which interact reciprocally to form a cage, as well as four FtsH hexamers with periplasmic domains and transmembrane helices enclosed inside the cage and cytoplasmic domains situated at the base of the cage. FtsH K61/D62/S63 in the β2-β3 loop in the periplasmic domain directly interact with HflK, contributing to complex formation. Pull-down and in vivo enzymatic activity assays validate the importance of the interacting interface for FtsH-HflKC complex formation. Structural comparison with the substrate-bound human m-AAA protease AFG3L2 offers implications for the HflKC cage in modulating substrate access to FtsH. Together, our findings provide a better understanding of FtsH-type AAA protease holoenzyme assembly and regulation. Ministry of Education (MOE) Published version This work was supported by a Tier II grant MOE2019-T2-2-099 and a Tier 1 grant RG108/20 from the Ministry of Education (MOE) of Singapore (Y.-G.G.).

Published
2022

23. Orchestrated actin nucleation by the Candida albicans polarisome complex enables filamentous growth

Author

Hongye Li, Shengyang Jin, Yinyue Deng, Yansong Miao, Zhu Qiao, Lanyuan Lu, Jiao Xue, Zhi Yang Loh, Yue Wang, Yong-Gui Gao, Feng Zhou, Jialin Sun, and Ying Xie

Subjects
0301 basic medicine, Hyphal growth, Polarisome, 030102 biochemistry & molecular biology, biology, Chemistry, Saccharomyces cerevisiae, Nucleation, macromolecular substances, Cell Biology, biology.organism_classification, Biochemistry, Actins, Polymerization, Cell biology, Fungal Proteins, 03 medical and health sciences, 030104 developmental biology, Formins, Candida albicans, Cell polarity, biology.protein, Molecular Biology, Actin, Actin nucleation
Abstract

Candida albicans is a dimorphic fungus that converts from a yeast form to a hyphae form during infection. This switch requires the formation of actin cable to coordinate polarized cell growth. It's known that nucleation of this cable requires a multiprotein complex localized at the tip called the polarisome, but the mechanisms underpinning this process were unclear. Here, we found that C. albicans Aip5, a homolog of polarisome component ScAip5 in Saccharomyces cerevisiae that nucleates actin polymerization and synergizes with the formin ScBni1, regulates actin assembly and hyphae growth synergistically with other polarisome proteins Bni1, Bud6, and Spa2. The C terminus of Aip5 binds directly to G-actin, Bni1, and the C-terminal of Bud6, which form the core of the nucleation complex to polymerize F-actin. Based on insights from structural biology and molecular dynamic simulations, we propose a possible complex conformation of the actin nucleation core, which provides cooperative positioning and supports the synergistic actin nucleation activity of a tri-protein complex Bni1-Bud6-Aip5. Together with known interactions of Bni1 with Bud6 and Aip5 in S. cerevisiae, our findings unravel molecular mechanisms of C. albicans by which the tri-protein complex coordinates the actin nucleation in actin cable assembly and hyphal growth, which is likely a conserved mechanism in different filamentous fungi and yeast.

Published
2020

24. Structural and computational examination of the Arabidopsis profilin–Poly-P complex reveals mechanistic details in profilin-regulated actin assembly

Author

Yansong Miao, Si Hui Koh, Qianqian Ma, Yuguang Mu, He Sun, Yong-Gui Gao, Justin Tze Yang Ng, and Zhu Qiao

Subjects
0301 basic medicine, 030102 biochemistry & molecular biology, biology, Molecular model, Chemistry, macromolecular substances, Cell Biology, biology.organism_classification, Biochemistry, 03 medical and health sciences, 030104 developmental biology, Profilin, Plant protein, Formins, Arabidopsis, biology.protein, Biophysics, Molecular Biology, Actin, Actin nucleation, Polyproline helix
Abstract

Profilins are abundant cytosolic proteins that are universally expressed in eukaryotes and that regulate actin filament elongation by binding to both monomeric actin (G-actin) and formin proteins. The atypical profilin Arabidopsis AtPRF3 has been reported to cooperate with canonical profilin isoforms in suppressing formin-mediated actin polymerization during plant innate immunity responses. AtPRF3 has a 37-amino acid-long N-terminal extension (NTE), and its suppressive effect on actin assembly is derived from enhanced interaction with the polyproline (Poly-P) of the formin AtFH1. However, the molecular mechanism remains unclear. Here, we solved the crystal structures of AtPRF3Δ22 and AtPRF3Δ37, as well as AtPRF2 apo form and in complex with AtFH1 Poly-P at 1.5-3.6 A resolutions. By combining these structures with molecular modeling, we found that AtPRF3Δ22 NTE has high plasticity, with a primary "closed" conformation that can adopt an open conformation that enables Poly-P binding. Furthermore, using molecular dynamics simulation and free-energy calculations of protein-protein binding, along with experimental validation, we show that the AtPRF3Δ22 binds to Poly-P in an adaptive manner, thereby enabling different binding modes that maintain the interaction through disordered sequences. Together, our structural and simulation results suggest that the dynamic conformational changes of the AtPRF3 NTE upon Poly-P binding modulate their interactions to fine-tune formin-mediated actin assembly.

Published
2019

25. Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway

Author

Ying, Zhang, Changjun, Yin, Chao, Wei, Shuai, Xia, Zhu, Qiao, Xue-Wen, Zhang, Bin, Yu, Jun, Zhou, and Rong, Wang

Subjects
Pharmacology, Epithelial-Mesenchymal Transition, Tumor Suppressor Proteins, RNA-Binding Proteins, Nerve Tissue Proteins, Gene Expression Regulation, Neoplastic, MicroRNAs, Cancer-Associated Fibroblasts, WW Domain-Containing Oxidoreductase, Cell Line, Tumor, Tumor Microenvironment, Humans, CELF Proteins, Colorectal Neoplasms, Cell Proliferation
Abstract

Migration, invasion, epithelial-mesenchymal transformation (EMT), and chemotherapeutic resistance are the leading causes of therapeutic failure in people with colorectal cancer (CRC). The migration of exosomal miRNA between cancer cells and the tumor microenvironment is directly associated with malignant behavior in cancer-associated fibroblasts (CAFs). In the context of earlier research, the purpose of the current study was to assess the role and potential mechanism of miR-625-3p released by CAFs in CRC cells. Exosomes were extracted and purified from CAFs conditioned medium by ultracentrifugation. Western blot, immunohistochemistry, CCK-8, transwell assay, HE staining, Tunnel, real-time PCR, double luciferase assay, RNA-binding protein immunoprecipitation (RIP), and immunofluorescence double staining experiments were used to investigate the effects of CAFs-Exo and miR-625-3p on CRC cell invasion, migration, proliferation, EMT, chemotherapeutic resistance, and molecular mechanisms. The current results indicated that CAFs-Exo was directly internalized by CRC cells, and exosomal miR-625-3p derived from CAFs might promote migration, invasion, EMT and chemotherapeutic resistance in CRC cells by inhibiting the CELF2/WWOX pathway, providing a potential candidate for CRC prediction and treatment.

Published
2022

37. A novel bacteriocin BMP11 and its antibacterial mechanism on cell envelope of Listeria monocytogenes and Cronobacter sakazakii

Author

Zhu Qiao, Lingli Luo, Lanhua Yi, Xin Lü, Hong Yan, Xin Li, and Yingying Lu

Subjects
0301 basic medicine, Lysis, biology, 030106 microbiology, Perforation (oil well), biology.organism_classification, medicine.disease_cause, Antimicrobial, Cronobacter sakazakii, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Bacteriocin, Listeria monocytogenes, chemistry, medicine, Propidium iodide, Cell envelope, Food Science, Biotechnology
Abstract

Listeria monocytogenes and Cronobacter sakazakii are notorious pathogens involved in numerous foodborne outbreaks after ingested contaminated food. Bacteriocins are natural food preservatives, some of which have antimicrobial activity comparable with antibiotics. In this study, a plasmid encoded novel bacteriocin BMP11 produced by Lactobacillus crustorum MN047 was innovatively identified by combining complete genome and LC-MS/MS. The BMP11 was found to have rich α-helix conformation after prediction. Moreover, the antimicrobial activity of BMP11 was verified after its heterologous expression in E. coli with 1280 and 640 AU/mL against L. monocytogenes and C. sakazakii, respectively. After purification by anion-exchange chromatography and HPLC, BMP11 had MIC values of 0.3–38.4 μg/mL against tested foodborne pathogens. Further, it was found that BMP11 had bactericidal action mode with concomitant cell lysis to pathogens by growth curve and time-kill kinetics. The results of scanning electron microscope (SEM) and transmission electron microscope (TEM) indicated that BMP11 destroyed the integrity of cell envelope of pathogens with cell wall perforation and cell membrane permeabilization. The destruction of cell envelope integrity was further verified by propidium iodide (PI) uptake and lactic dehydrogenase (LDH) release. BMP11 increased inner-membrane permeability of C. sakazakii in a concentration-dependent manner. Meanwhile, BMP11 exhibited antibiofilm formation activity. In addition, BMP11 inhibited the growth of L. monocytogenes in milk. Therefore, BMP11 had promising potential as antimicrobial to control foodborne pathogens in dairy products.

Published
2018

38. Crystal structure of a chitinase (RmChiA) from the thermophilic fungus Rhizomucor miehei with a real active site tunnel

Author

Yong-Gui Gao, Song-Qing Hu, Yuchun Liu, Zhu Qiao, Qiaojuan Yan, Shaoqing Yang, Junwen Ma, and Zhengqiang Jiang

Subjects
Stereochemistry, Mutant, Biophysics, Rhizomucor miehei, medicine.disease_cause, Biochemistry, Analytical Chemistry, Triosephosphate isomerase, Fungal Proteins, Catalytic Domain, Enzyme Stability, medicine, Rhizomucor, Molecular Biology, Escherichia coli, Thermostability, chemistry.chemical_classification, biology, Hydrolysis, Chitinases, Active site, biology.organism_classification, Amino acid, chemistry, Mutation, Chitinase, biology.protein
Abstract

A chitinase gene (RmChiA) encoding 445 amino acid (aa) residues from a fungus Rhizomucor miehei was cloned and overexpressed in Escherichia coli. Two kinds of RmChiA crystal forms, with space groups P32 2 1 and P1, were obtained by sitting-drop vapor diffusion and the structures were determined by X-ray diffraction. The overall structure of RmChiA monomer, which is the first structure of bacterial-type chitinases from nonpathogenic fungi, adopts a canonical triosephosphate isomerase (TIM) barrel fold with two protruding chitinase insertion domains. RmChiA exhibited a unique NxDxE catalytical motif and a real active site tunnel structure, which are firstly found in GH family 18 chitinases. The motif had high structural homolog with the typical DxDxE motif in other GH family 18 chitinases. The tunnel is formed by two unusual long loops, containing 15 aa and 45 aa respectively, linked by a disulfide bond across the substrate-binding cleft. Mutation experiments found that opening the roof of tunnel structure increased the hydrolysis efficiency of RmChiA, but the thermostability of the mutants decreased. Moreover, the tunnel structure endowed RmChiA with the exo-chitinase character.

Published
2021

47. Cross-polarization conversion with periodic perforated silver film in the near-infrared spectral range

Author

Hong-Mei Gong, Tie-Zhu Qiao, and Wei-bang Ji

Subjects
Materials science, business.industry, Near-infrared spectroscopy, Surface plasmon, Physics::Optics, Oblique case, Extraordinary optical transmission, 02 engineering and technology, 021001 nanoscience & nanotechnology, Polarization (waves), 01 natural sciences, Waveplate, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Wavelength, Optics, Electric field, 0103 physical sciences, Electrical and Electronic Engineering, 010306 general physics, 0210 nano-technology, business
Abstract

An enhanced polarization rotation of the zero-order transmission in the near-infrared spectral range is realized by using a silver film with an array of perforated normal and oblique rectangular holes. The ellipticity is small when the phase difference between E x and E y components approaches to π, and the transmitted field is nearly linear polarized within a wide spectral range. The polarization of transmitted field is determined by the intensity ratio between x and y components of electric field, which is modified by manipulating the incident wavelength or the incident polarization. Without changing the geometry parameters, one can get any desired rotation angle using the designed structure. In addition, the transmission is strong, and the designed structure can be used as a subwavelength half wave plate.

Published
2016

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