Search

Objective to assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and to evaluate their diagnostic potential to replace the old conventional method in animal ascetic fluid. Methods Highly reactive and specific IgM MAb secreting hybridoma cell line to S. mansoni SEA was cultured in three different media; serum rich replacement medium (conventional), serum low replacement medium (SLRM) and serum free replacement medium (SFRM). Results Cell count and viability were better in conventional medium and SLRM than SFRM. The lower detection limit of the assay Mab of SLRM culture was 1.1 ng/ml of S. mansoni SEA. SFRM was excluded because of the low concentration and reactivity. MAb was tested against 34 human samples. Sensitivity and specificity of the assay for each were 92% and specificity 90% for SLRM respectively. The diagnostic efficacy of the assay was 94%. Conclusions the production and diagnostic efficacy of antischistosomal MAbs was comparable on usage of either in vitro cell culture supernate in SLRM or conventional media.

OL-021 Echinococcosis of children in Bosnia and Herzegovina A. Bajraktarevic1 *, S. Trhulj Putica1, S. Trninic1, A. Skopljak1, N. Dizdarevic Kreso1, B. Djukic2, A. Hadzimuratovic Jr.3, E. Mujicic Selimovic4, A. Drnda5, Z. Jatic6, A. Hadzimuratovic7, J. Gutic8, M. Ridzal8. 1Public Health Institution of Health Center Sarajevo Pediatrics Department, 2First Medical Aid New Sarajevo, Pediatrics Department, 3Pediatrics Clinic Sarajevo, 4Clinical Medical Center Sarajevo, 5Infective Clinic Sarajevo, 6Medical Faculty of Sarajevo, 7Children Surgery Sarajevo, 8General Hospital Sarajevo, Bosnia and Herzegovina