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Your search keyword '"Yoshitake, Kazutoshi"' showing total 9 results
Start Over Author "Yoshitake, Kazutoshi" Publisher elsevier bv
9 results on '"Yoshitake, Kazutoshi"'

1. A homozygous structural variant of RPGRIP1 is frequently associated with achromatopsia in Japanese patients with IRD

Author

Suga, Akiko, primary, Mizobuchi, Kei, additional, Inooka, Taiga, additional, Yoshitake, Kazutoshi, additional, Minematsu, Naoko, additional, Tsunoda, Kazushige, additional, Kuniyoshi, Kazuki, additional, Kawai, Yosuke, additional, Omae, Yosuke, additional, Tokunaga, Katsushi, additional, Ishibashi-Ueda, Hatsue, additional, Tomita, Tsutomu, additional, Noguchi, Michio, additional, Takahashi, Ayako, additional, Goto, Yu-ichi, additional, Yoshida, Sumiko, additional, Hattori, Kotaro, additional, Matsumura, Ryo, additional, Iida, Aritoshi, additional, Maruoka, Yutaka, additional, Gatanaga, Hiroyuki, additional, Sugiyama, Masaya, additional, Suzuki, Satoshi, additional, Miyo, Kengo, additional, Matsubara, Yoichi, additional, Umezawa, Akihiro, additional, Hata, Kenichiro, additional, Kaname, Tadashi, additional, Ozaki, Kouichi, additional, Tokuda, Haruhiko, additional, Watanabe, Hiroshi, additional, Niida, Shumpei, additional, Noiri, Eisei, additional, Kitajima, Koji, additional, Miyahara, Reiko, additional, Shimanuki, Hideyuki, additional, Hayashi, Takaaki, additional, Ueno, Shinji, additional, and Iwata, Takeshi, additional

Published
2024
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7. Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori

Author

Tomihara, Kenta, primary, Satta, Katsuya, additional, Matsuzaki, Shohei, additional, Yoshitake, Kazutoshi, additional, Yamamoto, Kimiko, additional, Uchiyama, Hironobu, additional, Yajima, Shunsuke, additional, Futahashi, Ryo, additional, Katsuma, Susumu, additional, Osanai-Futahashi, Mizuko, additional, and Kiuchi, Takashi, additional

Published
2021
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9. Dimerization-based homogeneous fluorosensor proteins for the detection of specific dsDNA

Author

Yoshitake, Kazutoshi, Waki, Shoko, and Ueda, Hiroshi

Subjects
Phage display, Inverted repeat, Molecular Sequence Data, Analytical chemistry, Biomedical Engineering, Biophysics, chemistry.chemical_element, Biosensing Techniques, Zinc, Green fluorescent protein, chemistry.chemical_compound, Metalloproteins, Fluorescence Resonance Energy Transfer, Electrochemistry, skin and connective tissue diseases, Fluorescent Dyes, Repetitive Sequences, Nucleic Acid, Detection limit, Zinc finger, Base Sequence, Zinc Fingers, DNA, Sequence Analysis, DNA, General Medicine, Fluorescence, Molecular biology, DNA-Binding Proteins, Luminescent Proteins, Förster resonance energy transfer, chemistry, Dimerization, Biosensor, Biotechnology
Abstract

While there are many hybridization-based DNA sensors, few can detect native double-stranded DNA (dsDNA), which is most commonly found in physiological conditions. Here we made novel fluorosensor proteins comprised of a pair of two zinc fingers with an N-terminal dimerization motif and a C-terminal GFP variant to detect specific dsDNA sequence in a homogeneous solution. When a pair of purified zinc finger-GFP colour variant proteins (Zif12-eCFP, Zif12-eYFP) were mixed and added with specific dsDNA with 12 bp inverted repeat sequence, fluorescence spectra of the solution showed significant concentration-dependent enhancement of fluorescence resonance energy transfer (FRET), with the detection limit of approximately 10 nM. No significant change in FRET was observed upon addition of dsDNA with non-specific sequence, indicating dsDNA-dependent dimerization of the two proteins. This dimerization-based dsDNA sensors will have a range of applications where conventional hybridization-based assay is difficult.

Published
2008
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