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Start Over Author "Yi, Shounan" Publisher elsevier bv
10 results on '"Yi, Shounan"'

2. Regulatory T cells in kidney disease and transplantation

Author

Hu, Min, primary, Wang, Yuan Min, additional, Wang, Yiping, additional, Zhang, Geoff Y., additional, Zheng, Guoping, additional, Yi, Shounan, additional, O’Connell, Philip J., additional, Harris, David C.H., additional, and Alexander, Stephen I., additional

Published
2016
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3. Apoptosis detection by annexin V binding: a novel method for the quantitation of cell-mediated cytotoxicity

Author

Yi Shounan, Ximin Feng, and Philip J. O'Connell

Subjects
Cytotoxicity, Immunologic, Programmed cell death, Swine, Ligand binding assay, Immunology, Apoptosis, Phosphatidylserines, Annexin A5 affinity assay, Biology, Cytotoxicity Tests, Immunologic, Lymphocyte Activation, Molecular biology, Membrane Lipids, Phagocytosis, Annexin, Leukocytes, Mononuclear, Animals, Humans, Immunology and Allergy, Cytotoxic T cell, DNA fragmentation, Annexin A5, Cytotoxicity
Abstract

Current standard methods for the measurement of cell-mediated cytotoxicity rely on radioactive tracers, which either detect the release of cytoplasmic contents after plasma membrane disintegration by dying cells (51Cr release), or retained DNA by living cells (the JAM test). In this study, the annexin V binding assay of early apoptosis was applied to measure cell-mediated cytotoxicity. Primed human lymphocytes were examined for their ability to lyse either xenogeneic pig endothelial or allogeneic human PBMC target cells by assaying annexin V binding and the results compared with those obtained by the JAM test. Assaying annexin V binding by indirect immunofluorescence was demonstrated to be more sensitive and faster than the JAM test, which is a well-described, sensitive and simple assay for DNA fragmentation and cell death. However, the annexin V binding method was considered a more accurate measurement of absolute cytotoxicity as individual cell lysis was detected directly. In other methods, cytotoxic activity was calculated indirectly as a percentage of retained or released radioactive label. In addition, the apoptosis induced by the cell-mediated cytotoxicity can be visualized by this method thereby allowing a more accurate and sensitive quantitation of the number of apoptotic cells present when low effector to target ratios are used. These advantages make the annexin V binding method superior to other conventional cytotoxicity assays, particularly in situations where effector cells can be easily distinguished or separated from target cells.

Published
1998

4. Thyroid-stimulating hormone regulates hepatic bile acid homeostasis via SREBP-2/HNF-4α/CYP7A1 axis

Author

Song, Yongfeng, primary, Xu, Chao, additional, Shao, Shanshan, additional, Liu, Jun, additional, Xing, Wanjia, additional, Xu, Jin, additional, Qin, Chengkun, additional, Li, Chunyou, additional, Hu, Baoxiang, additional, Yi, Shounan, additional, Xia, Xuefeng, additional, Zhang, Haiqing, additional, Zhang, Xiujuan, additional, Wang, Tingting, additional, Pan, Wenfei, additional, Yu, Chunxiao, additional, Wang, Qiangxiu, additional, Lin, Xiaoyan, additional, Wang, Laicheng, additional, Gao, Ling, additional, and Zhao, Jiajun, additional

Published
2015
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