1. Cancer-targeted PEDF-DNA therapy for metastatic colorectal cancer
- Author
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Fazhan Wang, Jun Zeng, Lei Wang, Xiangrong Song, Hai Huang, Xuelian Liao, Xingting Bao, and Cui-Cui Ma
- Subjects
Male ,Lung Neoplasms ,Receptors, Peptide ,Colorectal cancer ,Genetic enhancement ,Pharmaceutical Science ,Apoptosis ,Cell-Penetrating Peptides ,02 engineering and technology ,030226 pharmacology & pharmacy ,Metastasis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,PEDF ,Cell Movement ,Cell Line, Tumor ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,Nerve Growth Factors ,Receptors, Immunologic ,Eye Proteins ,Serpins ,Mice, Inbred BALB C ,Lung ,business.industry ,Cancer ,021001 nanoscience & nanotechnology ,medicine.disease ,In vitro ,Tumor Burden ,Clinical trial ,medicine.anatomical_structure ,Gene Targeting ,Cancer research ,Colorectal Neoplasms ,0210 nano-technology ,business ,Oligopeptides - Abstract
Colorectal cancer (CRC) is a major cause of cancer-related mortality worldwide. Moreover, metastasis is one of the main causes of death in CRC patients. Nanotechnology-based gene therapy has shown significant therapeutic benefits in recent clinical trials for cancer treatment. Recent studies have shown that pigment epithelium-derived factor (PEDF) protein can inhibit tumor growth and metastasis by anti-angiogenesis and pro-apoptosis. In this study, we prepared a PEDF-DNA-loaded liposome for cancer-targeted gene therapy for metastatic CRC using an iRGD peptide. Our results showed that cancer-targeted PEDF-DNA liposomes (R-LP/PEDF) exhibited enhanced inhibitory effects on invasion, migration, and pro-apoptosis of CRC cells in vitro. In addition, it reduced metastasis tumor nodules in lung and prolonged the survival time in a mouse model of metastatic CRC.
- Published
- 2020
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