1. Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model
- Author
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Yinghe Hu, Qiang Sun, Xiaoluan Wei, Pu Wu, Qian Shen, Jie Feng, Hou-Da Li, Juan Dong, Yujuan Jin, and Rong Zhang
- Subjects
Blood Glucose ,Genetically modified mouse ,medicine.medical_specialty ,Antigens, Polyomavirus Transforming ,Transgene ,Mice, Transgenic ,Bioengineering ,Biology ,Applied Microbiology and Biotechnology ,Mice ,Western blot ,Somatomedins ,Internal medicine ,medicine ,Animals ,Glucose test ,geography ,geography.geographical_feature_category ,Oncogene ,medicine.diagnostic_test ,Pancreatic islets ,Tetracycline ,Phosphoproteins ,Islet ,Pancreatic Neoplasms ,Disease Models, Animal ,Neuroendocrine Tumors ,medicine.anatomical_structure ,Endocrinology ,Insulin Receptor Substrate Proteins ,Cancer research ,Insulinoma ,Pancreas ,Biotechnology - Abstract
A line of double-transgenic mice that develop neoplasms arising primarily in the pancreas was established. In these mice, the oncogene SV40 T antigen (Tag) was detected in the pancreas with and without the control of Tet-on system. The transgenic mice that developed pancreatic tumors as early as 20 weeks of age showed hypoglycemia on a blood glucose test. Pathological and immunohistochemical characterizations demonstrated that the tumors belonged to neuroendocrine neoplasms arising from pancreatic islets. A change in IGFs/IGF-1R signaling pathway was detected using real-time PCR analysis. A potential association between the IGFs/IGF-1R system and SV40Tag was studied to further explain the cancerogenesis of the double-transgenic mice by Western blot analysis and immunoprecipitation experiments. The results suggest that a Tag transgenic mice model could be used to study the molecular mechanism of the tumorigenesis of islets.
- Published
- 2007