1. Bortezomib Facilitates Reparative Dentin Formation after Pulp Access Cavity Preparation in Mouse Molar
- Author
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Jae-Kwang Jung, Wern-Joo Sohn, Ki-Rim Kim, Tae-Yub Kwon, Seo-Young An, Gi-Jeong Gwon, Jae-Young Kim, Jung-Hong Ha, Chang-Hyeon An, Ji-Youn Kim, Youngkyun Lee, and Sanjiv Neupane
- Subjects
Male ,0301 basic medicine ,Dentistry ,Bone morphogenetic protein ,Bortezomib ,Mice ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,medicine ,Animals ,General Dentistry ,Mice, Inbred ICR ,Chemistry ,business.industry ,Wnt signaling pathway ,030206 dentistry ,Nestin ,Molar ,Molecular biology ,Pulp capping ,030104 developmental biology ,Proteasome ,Dentin ,Proteasome inhibitor ,Pulp (tooth) ,Dental Cavity Preparation ,business ,medicine.drug - Abstract
Introduction The aim of this study was to evaluate in vitro and ex vivo roles of bortezomib, a proteasome inhibitor that binds to the active site of the 26S proteasome, in tertiary dentin formation. Methods We established pulpal access cavity preparation that was treated with or without bortezomib before direct pulp capping with a calcium hydroxide–based material. We also analyzed bone morphogenetic protein (Bmp)- and Wnt-related signaling molecules using quantitative real-time polymerase chain reaction. Results In the short-term observation period, the bortezomib-treated pulp specimens showed the period-altered immunolocalization patterns of nestin, CD31, and myeloperoxidase, whereas the control specimens did not. The bortezomib-treated group showed a complete dentin bridge with very few irregular tubules after 42 days. The micro–computed tomographic images showed more apparent dentin bridge structures in the treated specimens than were in the controls. Quantitative real-time polymerase chain reaction analysis showed up-regulated Bmp and Wnt. Conclusions These findings revealed that treatment with 1 μmol/L bortezomib induced reparative dentin formation that facilitated the maintenance of the integrity of the remaining pulpal tissue via early vascularization and regulation of Bmp and Wnt signaling.
- Published
- 2017