1. Comparison of point-of-care test and enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies in the diagnosis of human schistosomiasis japonica
- Author
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Patcharaporn Boonroumkaew, Wanchai Maleewong, Tongjit Thanchomnang, Hiroshi Yamasaki, Phiraphol Chusongsang, Hiroshi Ohmae, Oranuch Sanpool, Yanin Limpanont, Lakkhana Sadaow, Pewpan M. Intapan, Weeraya Phupiewkham, and Rutchanee Rodpai
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Asia ,Concordance ,Point-of-care testing ,030106 microbiology ,Antibodies, Helminth ,Enzyme-Linked Immunosorbent Assay ,Schistosomiasis ,Infectious and parasitic diseases ,RC109-216 ,Sensitivity and Specificity ,Gastroenterology ,Schistosoma japonicum ,Immunoglobulin G ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Predictive Value of Tests ,Positive predicative value ,Internal medicine ,parasitic diseases ,Animals ,Humans ,Medicine ,030212 general & internal medicine ,Point-of-care test ,Immunoassay ,Serodiagnosis ,biology ,business.industry ,General Medicine ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Point-of-Care Testing ,Adult somatic antigen ,Schistosomiasis japonica ,biology.protein ,Female ,Antibody ,business ,Immunochromatographic test kit - Abstract
Objective Schistosomiasis japonica is an important helminthic disease in Asia. Sensitive and accurate diagnostic tools are indispensable for clinical diagnosis, screening infection and monitoring its control. In this study, we developed an immunochromatographic test (Sj-ICT) to detect anti-Schistosoma japonicum immunoglobulin G antibodies in human sera. Methods Somatic extract from adult S. japonicum was used as an antigen. The Sj-ICT was developed and optimized as a point-of-care test. All 214 human serum samples were evaluated for diagnostic usefulness and comparison with an enzyme-linked immunosorbent assay (ELISA). Results The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy of the Sj-ICT were 90.8%, 87.9%, 86.4%, 91.9% and 89.3%, respectively. For ELISA the values were respectively 91.8%, 87.9%, 86.5%, 92.7% and 89.7%. The concordance between both methods was 86.4 % (Cohen’s kappa value = 0.729). Conclusions The immunochromatographic test kit developed can support clinical diagnosis and large-scale surveys in endemic areas without requiring additional facilities or ancillary supplies.
- Published
- 2021