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Your search keyword '"Weber, Franz"' showing total 39 results
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39 results on '"Weber, Franz"'

2. SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model

Author

Casanova, Elisa A., primary, Rodriguez-Palomo, Adrian, additional, Stähli, Lisa, additional, Arnke, Kevin, additional, Gröninger, Olivier, additional, Generali, Melanie, additional, Neldner, Yvonne, additional, Tiziani, Simon, additional, Dominguez, Ana Perez, additional, Guizar-Sicairos, Manuel, additional, Gao, Zirui, additional, Appel, Christian, additional, Nielsen, Leonard C., additional, Georgiadis, Marios, additional, Weber, Franz E., additional, Stark, Wendelin, additional, Pape, Hans-Christoph, additional, Cinelli, Paolo, additional, and Liebi, Marianne, additional

Published
2023
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15. Accuracy of Cuff-Measured Blood Pressure

Author

Picone, Dean S., primary, Schultz, Martin G., additional, Otahal, Petr, additional, Aakhus, Svend, additional, Al-Jumaily, Ahmed M., additional, Black, J. Andrew, additional, Bos, Willem J., additional, Chambers, John B., additional, Chen, Chen-Huan, additional, Cheng, Hao-Min, additional, Cremer, Antoine, additional, Davies, Justin E., additional, Dwyer, Nathan, additional, Gould, Brian A., additional, Hughes, Alun D., additional, Lacy, Peter S., additional, Laugesen, Esben, additional, Liang, Fuyou, additional, Melamed, Roman, additional, Muecke, Sandy, additional, Ohte, Nobuyuki, additional, Okada, Sho, additional, Omboni, Stefano, additional, Ott, Christian, additional, Peng, Xiaoqing, additional, Pereira, Telmo, additional, Pucci, Giacomo, additional, Rajani, Ronak, additional, Roberts-Thomson, Philip, additional, Rossen, Niklas B., additional, Sueta, Daisuke, additional, Sinha, Manish D., additional, Schmieder, Roland E., additional, Smulyan, Harold, additional, Srikanth, Velandai K., additional, Stewart, Ralph, additional, Stouffer, George A., additional, Takazawa, Kenji, additional, Wang, Jiguang, additional, Westerhof, Berend E., additional, Weber, Franz, additional, Weber, Thomas, additional, Williams, Bryan, additional, Yamada, Hirotsugu, additional, Yamamoto, Eiichiro, additional, and Sharman, James E., additional

Published
2017
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20. Enzymatic Formation of Modular Cell-Instructive Fibrin Analogs for Tissue Engineering

Author

Ehrbar, Martin, Rizzi, Simone, Hlushchuk, Ruslan, Djonov, Valentin, Zisch, Andreas, Hubbell, Jeffrey, Weber, Franz, Lutolf, Matthias, Ehrbar, Martin, Rizzi, Simone, Hlushchuk, Ruslan, Djonov, Valentin, Zisch, Andreas, Hubbell, Jeffrey, Weber, Franz, and Lutolf, Matthias

Abstract

The molecular engineering of cell-instructive artificial extracellular matrices is a powerful means to control cell behavior and enable complex processes of tissue formation and regeneration. This work reports on a novel method to produce such smart biomaterials by recapitulating the crosslinking chemistry and the biomolecular characteristics of the biopolymer fibrin in a synthetic analog. We use activated coagulation transglutaminase factor XIIIa for site-specific coupling of cell adhesion ligands and engineered growth factor proteins to multiarm poly(ethylene glycol) macromers that simultaneously form proteolytically sensitive hydrogel networks in the same enzyme-catalyzed reaction. Growth factor proteins are quantitatively incorporated and released upon cell-derived proteolytic degradation of the gels. Primary stromal cells can invade and proteolytically remodel these networks both in an in vitro and in vivo setting. The synthetic ease and potential to engineer their physicochemical and bioactive characteristics makes these hybrid networks true alternatives for fibrin as provisional drug delivery platforms in tissue engineering.

Published
2007

37. The epigenetically active small chemical N-methyl pyrrolidone (NMP) prevents estrogen depletion induced osteoporosis

Author

Franz E. Weber, Bebeka Gjoksi, Barbara Siegenthaler, Marcy Zenobi-Wong, Chafik Ghayor, Nisarat Ruangsawasdi, University of Zurich, and Weber, Franz E

Subjects
Physiology, Endocrinology, Diabetes and Metabolism, Osteoporosis, Osteoclasts, 2722 Histology, Epigenesis, Genetic, Bone remodeling, Rats, Sprague-Dawley, 0302 clinical medicine, Bone Density, Femur, Bone mineral, 0303 health sciences, Chemistry, Cell Differentiation, Pyrrolidinones, 3. Good health, 2712 Endocrinology, Diabetes and Metabolism, medicine.anatomical_structure, 10076 Center for Integrative Human Physiology, 030220 oncology & carcinogenesis, Ovariectomized rat, Female, Bone Remodeling, medicine.medical_specialty, Histology, Ovariectomy, 610 Medicine & health, Bone morphogenetic protein, Bone and Bones, Bone resorption, 03 medical and health sciences, Imaging, Three-Dimensional, Osteoclast, Internal medicine, medicine, Animals, Bone Resorption, Bone regeneration, 030304 developmental biology, Osteoblasts, Body Weight, Estrogens, X-Ray Microtomography, 1314 Physiology, Alkaline Phosphatase, medicine.disease, Protein Structure, Tertiary, Rats, Endocrinology, Microscopy, Fluorescence, 10069 Clinic of Cranio-Maxillofacial Surgery
Abstract

Currently, there are several treatments for osteoporosis however; they all display some sort of limitation and/or side effects making the need for new treatments imperative. We have previously demonstrated that NMP is a bioactive drug which enhances bone regeneration in vivo and acts as an enhancer of bone morphogenetic protein (BMP) in vitro. NMP also inhibits osteoclast differentiation and attenuates bone resorption. In the present study, we tested NMP as a bromodomain inhibitor and for osteoporosis prevention on ovariectomized (OVX) induced rats while treated systemically with NMP. Female Sprague-Dawley rats were ovariectomized and weekly NMP treatment was administrated 1 week after surgery for 15 weeks. Bone parameters and related serum biomarkers were analyzed. 15 weeks of NMP treatment decreased ovariectomy-induced gained weight in average by 43% and improved bone mineral density (BMD) and bone volume over total volume (BV/TV) in rat femur on average by 25% and 41% respectively. Moreover, mineral apposition rate and bone biomarkers of bone turnover in the treatment group were at similar levels with those of the Sham group. Due to the function of NMP as a low affinity bromodomain inhibitor and its mechanism of action involving osteoblasts/osteoclasts balance and inhibitory effect on inflammatory cytokines, NMP is a promising therapeutic compound for the prevention of osteoporosis.

Published
2015

38. N-methyl pyrrolidone/bone morphogenetic protein-2 double delivery with in situ forming implants

Author

Michael de Wild, Franz E. Weber, Barbara Siegenthaler, Jean-Christophe Leroux, Chafik Ghayor, Lindsay S. Karfeld-Sulzer, University of Zurich, and Weber, Franz E

Subjects
medicine.medical_specialty, 3003 Pharmaceutical Science, Bone Morphogenetic Protein 2, Pharmaceutical Science, 610 Medicine & health, Bone morphogenetic protein, Bone morphogenetic protein 2, chemistry.chemical_compound, Osteogenesis, Transforming Growth Factor beta, In vivo, medicine, Animals, Bone regeneration, Polyglactin 910, Drug Implants, Lactide, Regeneration (biology), Skull, Pyrrolidinones, Recombinant Proteins, In vitro, Surgery, Cell biology, chemistry, 10076 Center for Integrative Human Physiology, Rabbits, Implant, 10069 Clinic of Cranio-Maxillofacial Surgery
Abstract

Bone morphogenetic proteins (BMPs) are growth and differentiation factors involved during development in morphogenesis, organogenesis and later mainly in regeneration processes, in particular in bone where they are responsible for osteoinduction. For more than a decade, recombinant human (rh)BMP-2 has been used in the clinic for lumbar spinal fusion at non-physiological high dosages that appear to be causative for side effects, like male sterility. A possible strategy to reduce the effective amount of rhBMP-2 in the clinic is the co-delivery with an enhancer of BMPs' activity. In an earlier study, we showed that N-methylpyrrolidone (NMP) enhances BMP activity in vitro and in vivo. Here we report on the development of a slow and sustained double delivery of rhBMP-2 and NMP via an in situ forming implant based on poly(lactide-co-glycolide). The results showed that the release of NMP can be adjusted by varying the lactide/glycolide ratio and the polymer's molecular weight. The same applied to rhBMP-2, with release rates that could be sustained from two to three weeks. In the in vivo model of a critical size defect in the calvarial bone of rabbits, the implant containing 50mol% lactide performed better than the one having 75mol% lactide in terms of defect bridging and extent of bony regenerated area. In situ forming implants for the double delivery of the BMP enhancer NMP and rhBMP-2 appear to be promising delivery systems in bone regeneration.

Published
2015

39. Effect of cerium chloride application on fibroblast and osteoblast proliferation and differentiation

Author

Franz E. Weber, Patrick R. Schmidlin, Alexandre Tchouboukov, Florian J Wegehaupt, University of Zurich, and Weber, Franz E

Subjects
Bone Morphogenetic Protein 2, ALIZARIN RED, 610 Medicine & health, Chloride, Cell Line, 1307 Cell Biology, Andrology, Calcification, Physiologic, Chlorides, 10066 Clinic of Conservative and Preventive Dentistry, medicine, Humans, Fibroblast, Cytotoxicity, General Dentistry, Cell Proliferation, Analysis of Variance, Osteoblasts, Chemistry, Cell Differentiation, Osteoblast, Cerium, Cell Biology, General Medicine, Fibroblasts, Alkaline Phosphatase, 3500 General Dentistry, Staining, 10022 Division of Surgical Research, 2733 Otorhinolaryngology, medicine.anatomical_structure, Otorhinolaryngology, Biochemistry, Alkaline phosphatase, Analysis of variance, 10069 Clinic of Cranio-Maxillofacial Surgery, medicine.drug
Abstract

OBJECTIVE: This study investigated effects of cerium-chloride on fibroblast and osteoblast differentiation and proliferation. METHODS: MC3T3-E1 cells were plated for an alkaline phosphatase (ALP) activity test. On day 3, CeCl(3)-solutions (1, 5 or 10, w/v%) were added. After 10s, the solutions were aspirated and washed to remove residual CeCl(3). On day 6 ALP activity was determined. Cell activity and proliferation was assessed by thiazolyl blue tetrazolium dye reduction assay (MTT-test) also 3 days after exposure to the CeCl(3)-solutions. Calcium deposition by preosteoblastic cells was determined 4 weeks after the exposure of the cells by alizarin red staining. Furthermore, in all experiments the influence of adding rhBMP-2 was tested. Statistical analysis was performed by repeated-measures ANOVA using the post hoc Fisher least significant difference (LSD) test. Statistical significance was set at p < 0.05. RESULTS: Exposure to a Ce-solution of 1% or higher reduced ALP activity significantly. The addition of rhBMP-2 was able to elevate ALP activity above control level. MTT-test showed a significant decrease in cellular activity by 5% Ce or higher. The addition of rhBMP-2 had no positive effect. For human foreskin fibroblasts, exposure to even 10% Ce yielded a significant increase in cellular activity. Ce reduced calcium deposition to a level of below 50% of the control. The addition of rhBMP-2 restored mineral deposition to control levels for all Ce concentrations. CONCLUSION: CeCl(3) had a stimulating effect on fibroblasts but a depressing influence on osteoblasts. However, adding rhBMP-2 could compensate the latter influence.

Published
2012

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