1. Error analysis in titration microcalorimetry of biochemical systems
- Author
-
Harry P. Hopkins, Virginia Carey, Syed Zakir Ali, and Leland P. Vickers
- Subjects
Isothermal microcalorimetry ,Enthalpy ,Biophysics ,Analytical chemistry ,Indicator Dilution Techniques ,Calorimetry ,Bovine pancreatic ribonuclease ,Biochemistry ,Standard deviation ,Cytidine Monophosphate ,Animals ,Molecular Biology ,Propagation of uncertainty ,biology ,Chemistry ,Microchemistry ,food and beverages ,Ribonuclease, Pancreatic ,Cell Biology ,Calorimeter ,biology.protein ,Thermodynamics ,Cattle ,Titration ,Protein Binding - Abstract
A simplified method for titrations of biochemical systems is described as well as extensive error propagation through the data analysis. This work uses a Tronac Model 450 isoperibol titration calorimeter. Sample volumes of 2 ml are used and total heats of less than 5 mcal can be routinely measured. The binding of 3'-CMP to bovine pancreatic ribonuclease A is used to illustrate the methods. The binding enthalpy can be determined with a standard deviation of 1.5% and the free energy with a standard deviation of 2 to 3%.
- Published
- 1985