1. Multiple drug resistant, tumorigenic stem-like cells in oral cancer
- Author
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Tessy Thomas Malieckal, M. Radhakrishna Pillai, Vinitha Richard, Sree Narayanan Nair, Madhumathy G. Nair, T. R. Santhosh Kumar, and Paul Sebastian
- Subjects
Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,ATP Binding Cassette Transporter, Subfamily B ,Integrin beta Chains ,Neoplasm, Residual ,Carcinogenesis ,Mice, SCID ,Biology ,Aldehyde Dehydrogenase 1 Family ,Mice ,Side population ,Mice, Inbred NOD ,Cancer stem cell ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,Animals ,Humans ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Squamous Cell Carcinoma of Head and Neck ,Regeneration (biology) ,Mesenchymal stem cell ,Membrane Proteins ,Retinal Dehydrogenase ,Cancer ,medicine.disease ,Immunohistochemistry ,Xenograft Model Antitumor Assays ,Isoenzymes ,Oncology ,Drug Resistance, Neoplasm ,Head and Neck Neoplasms ,Tumor progression ,Cell culture ,Basigin ,Carcinoma, Squamous Cell ,Neoplastic Stem Cells ,Cancer research ,Mouth Neoplasms ,Stem cell - Abstract
An in vitro cell line model was established to exemplify tumor stem cell concept in oral cancer. We were able to identify CD147 expressing fractions in SCC172 OSCC cell line with differing Hoechst dye efflux activity and DNA content. In vivo tumorigenic assay revealed three fractions enriched with stem-like cells capable of undergoing mesenchymal transition and a non-tumorigenic fraction. The regeneration potential and transition of one fraction to other imitated the phenotypic switch and functional disparities evidenced during oral tumor progression. Knowledge of these additional stem-like subsets will improve understanding of stem cell based oral epithelial tumor progression from normal to malignant lesions.
- Published
- 2013