1. Functional potential of sewage sludge digestate microbes to degrade aliphatic hydrocarbons during bioremediation of a petroleum hydrocarbons contaminated soil
- Author
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David Huguenot, Anna Gielnik, Giovanni Esposito, Eric D. van Hullebusch, Aurélie Cébron, Yoan Pechaud, Laboratoire Géomatériaux et Environnement (LGE), Université Paris-Est Marne-la-Vallée (UPEM), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio (UNICAS), Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), European Project: 643071,H2020,H2020-MSCA-ITN-2014,ABWET(2015), Gielnik, A., Pechaud, Y., Huguenot, D., Cebron, A., Esposito, G., van Hullebusch, E. D., Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut de Physique du Globe de Paris (IPGP (UMR_7154))
- Subjects
Organic wastes ,Environmental Engineering ,alkB gene ,PHC ,0208 environmental biotechnology ,Bioreactor ,AlkB ,02 engineering and technology ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,engineering.material ,complex mixtures ,01 natural sciences ,12. Responsible consumption ,Soil ,Bioreactors ,Bioremediation ,alkB genes ,Biochar ,Soil Pollutants ,Waste Management and Disposal ,Soil Microbiology ,Organic waste ,0105 earth and related environmental sciences ,2. Zero hunger ,Sewage ,biology ,[SDE.IE]Environmental Sciences/Environmental Engineering ,Chemistry ,PHCs ,General Medicine ,Biodegradable waste ,15. Life on land ,Soil contamination ,Hydrocarbons ,6. Clean water ,020801 environmental engineering ,Biodegradation, Environmental ,Petroleum ,13. Climate action ,Environmental chemistry ,Digestate ,biology.protein ,engineering ,Soil clean-up ,Fertilizer ,Sludge - Abstract
International audience; Sewage sludge digestate is a valuable organic waste which can be used as fertilizer in soil bioremediation. Sewage sludge digestate is not only a good source of nutrients but is also rich in bacteria carrying alkB genes, which are involved in aliphatic hydrocarbons metabolism. Increase of alkB genes ratio in polluted soils has been observed to improve bioremediation efficiency. In this study, for the first time, the genetic potential of indigenous microorganisms of digestate to degrade petroleum products was assessed. The objectives were to study petroleum hydrocarbons (PHCs) removal together with shifts in soil taxa and changes in the concentration of alkB genes after digestate application. Initial alkB genes concentration in contaminated soils and digestate was 1.5% and 4.5%, respectively. During soil incubation with digestate, alkB genes percentage increased up to 11.5% and after the addition of bacteria immobilized onto biochar this value increased up to 60%. Application of digestate positively affected soil respiration and bacterial density, which was concomitant with enhanced PHCs degradation. Incubation of soil amended with digestate resulted in 74% PHCs decrease in 2 months, while extra addition of bacteria immobilized onto biochar increased this value up to 95%. The use of digestate affected the microbial community profiles by increasing initial bacterial density and diversity, including taxa containing recognized PHCs degraders. This study reveals the great potential of digestate as a soil amendment which additionally improves the abundance of alkB genes in petroleum contaminated soils.
- Published
- 2021
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