1. Translational misreading in Mycobacterium smegmatis increases in stationary phase
- Author
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Xin Xu, Babak Javid, Tianqi Leng, and Miaomiao Pan
- Subjects
Microbiology (medical) ,Genotype ,Mycobacterium smegmatis ,Immunology ,Lysine ,medicine.disease_cause ,Microbiology ,Exponential growth ,Genes, Reporter ,Luciferases, Firefly ,medicine ,Luciferase ,Codon ,Escherichia coli ,Genetics ,Escherichia coli K12 ,biology ,Gene Expression Regulation, Bacterial ,biology.organism_classification ,Oxidative Stress ,Infectious Diseases ,Stationary phase ,Protein Biosynthesis ,Codon usage bias ,Mutation ,Streptomycin ,RNA, Transfer, Lys ,Mycobacterium - Abstract
The study of errors in gene translation has largely been confined to a small number of model organisms. We have examined all possible misreading errors at a defined codon in Mycobacterium smegmatis. Using a dual-luciferase gain of function reporter system that employs a mutated essential lysine in firefly luciferase, we accurately quantified mistranslation errors. Overall, accuracy of gene translation was comparable with Escherichia coli at
- Published
- 2015
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