4 results on '"Takashi Sakatani"'
Search Results
2. Osteoclast-like giant cells in invasive breast cancer predominantly possess M2-macrophage phenotype
- Author
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Takashi Sakatani, Hiroyuki Takei, Keiko Yanagihara, Zenya Naito, Ryuji Ohashi, Shigeki Namimatsu, and Akira Shimizu
- Subjects
Adult ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Foreign-body giant cell ,Osteoclasts ,Estrogen receptor ,Breast Neoplasms ,Biology ,Giant Cells ,Pathology and Forensic Medicine ,Capillary Permeability ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Osteoclast ,medicine ,Humans ,Lymphangiogenesis ,CD68 ,Macrophages ,Cell Biology ,Middle Aged ,medicine.disease ,Phenotype ,030104 developmental biology ,medicine.anatomical_structure ,Giant cell ,030220 oncology & carcinogenesis ,Female ,Breast carcinoma ,CD163 - Abstract
Breast carcinoma with osteoclast-like giant cells (OGCs) is a rare tumor; however, their clinicopathological aspects remain unclear. We described the clinicopathological characteristics of 8 patients with breast carcinoma with OGCs. Immuno-phenotypes of the OGCs were comparatively examined with that of foreign body giant cells (FBGCs) in 4 cases of granulomatous reaction (GR) without cancerous elements. In most cancers, tumors displayed cribriform and tubular growth patterns. Three cases showed moderate to high nuclear grade, while all the other tumors had low nuclear grade. Six patients were estrogen receptor (ER) positive, while triple negative phenotype was identified in 2 patients. During the follow-up period, 1 patient had local recurrence of the tumor, and all the patients remained alive. All OGCs and FBGCs expressed CD68, a pan-macrophage marker. OGCs in all the breast cancers showed moderate to high expression of CD163 - a marker of M2-macrophage with pro-tumoral function - whereas its expression in FBGCs was low to moderate (p=0.04). CD86 - a marker of M1-macrophage with a tumoricidal activity - was positive in the OGCs of 3 breast cancers, and in the FBGCs of 3 GR cases (p=0.15). The expression of CD163 was significantly higher than that of CD86 in the OGCs of breast cancer (p
- Published
- 2018
3. Identification of CCDC6-RET Fusion in the Human Lung Adenocarcinoma Cell Line, LC-2/ad
- Author
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Hiroyuki Aburatani, Daisuke Matsubara, Takashi Sakatani, Yoshihiko Kanai, Shiori Ohara, Yoshinori Murakami, Sachiko Oguni, Hiroaki Kataoka, Masashi Fukayama, Tomoko Tamura, Toshiro Niki, Taichiro Yoshimoto, Shunsuke Endo, and Shumpei Ishikawa
- Subjects
Lung adenocarcinoma ,Pathology ,Lung Neoplasms ,Oncogene Proteins, Fusion ,endocrine system diseases ,Mice, SCID ,Vandetanib ,Immunoenzyme Techniques ,Mice ,Piperidines ,Mice, Inbred NOD ,Carcinoma, Non-Small-Cell Lung ,Antineoplastic Combined Chemotherapy Protocols ,Tumor Cells, Cultured ,Phosphorylation ,Reverse Transcriptase Polymerase Chain Reaction ,Gefitinib ,Blot ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,Oncology ,Female ,medicine.drug ,Pulmonary and Respiratory Medicine ,congenital, hereditary, and neonatal diseases and abnormalities ,endocrine system ,medicine.medical_specialty ,Blotting, Western ,Transplantation, Heterologous ,Adenocarcinoma ,Real-Time Polymerase Chain Reaction ,medicine ,Carcinoma ,Animals ,Humans ,RNA, Messenger ,neoplasms ,RET fusion ,Cell Proliferation ,Lung ,business.industry ,Proto-Oncogene Proteins c-ret ,medicine.disease ,Transplantation ,Cytoskeletal Proteins ,Cell culture ,Quinazolines ,Cancer research ,Cell line ,business - Abstract
Rearranged during transfection (RET) fusions have been newly identified in approximately 1% of patients with primary lung tumors. However, patient-derived lung cancer cell lines harboring RET fusions have not yet been established or identified, and therefore, the effectiveness of an RET inhibitor on lung tumors with endogenous RET fusion has not yet been studied. In this study, we report identification of CCDC6-RET fusion in the human lung adenocarcinoma cell line LC-2/ad. LC-2/ad showed distinctive sensitivity to the RET inhibitor, vandetanib, among 39 non–small lung cancer cell lines. The xenograft tumor of LC-2/ad showed cribriform acinar structures, a morphologic feature of primary RET fusion–positive lung adenocarcinomas. LC-2/ad cells could provide useful resources to analyze molecular functions of RET-fusion protein and its response to RET inhibitors.
- Published
- 2012
4. Epigenetic Heterogeneity at Imprinted Loci in Normal Populations
- Author
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Motonobu Katoh, Daisuke Wada, Benjamin Tycko, Masahide Ikeguchi, Chiga Okita, Makiko Meguro, Hisao Ito, Michelle Wei, Mitsuo Oshimura, Kohzoh Mitsuya, and Takashi Sakatani
- Subjects
Male ,Organic Cation Transport Proteins ,Population ,Biophysics ,Biology ,Autoantigens ,Polymerase Chain Reaction ,Biochemistry ,snRNP Core Proteins ,Genomic Imprinting ,Japan ,Insulin-Like Growth Factor II ,Leukocytes ,Humans ,Epigenetics ,Allele ,education ,Molecular Biology ,Gene ,Alleles ,Genetics ,education.field_of_study ,Genetic Variation ,Membrane Proteins ,DNA ,Cell Biology ,Ribonucleoproteins, Small Nuclear ,Phenotype ,Pedigree ,Gene Expression Regulation ,Expression quantitative trait loci ,Trait ,Female ,Genomic imprinting - Abstract
Genomic imprinting is the phenomenon by which the two alleles of certain genes are differentially expressed according to their parental origin. Extensive analysis of allelic expression at multiple imprinted loci in a normal population has not performed so far. In the present study, we examined the allelic expression pattern of three imprinted genes in a panel of 262 Japanese normal individuals. We observed differences in the extent of maintenance of allele-specific expression of the three genes. The allelic expression of small nuclear ribonucleoprotein N (SNRPN) was stringently regulated while that of multimembrane-spanning polyspecific transporter-like gene 1 (IMPT1) showed a large degree of variation. Significant biallelic expression of insulin-like growth factor II (IGF2) was observed in about 10% of normal individuals. Our findings add to the accumulating evidence for variable allelic expression at multiple loci in a normal human population. This epigenetic heterogeneity can be a stable trait and potentially influence individual phenotypes.
- Published
- 2001
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