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Your search keyword '"Surani, M. Azim"' showing total 42 results
Start Over Author "Surani, M. Azim" Publisher elsevier bv
42 results on '"Surani, M. Azim"'

3. Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells

Author

Kobayashi, Toshihiro, primary, Castillo-Venzor, Aracely, additional, Penfold, Chris A., additional, Morgan, Michael, additional, Mizuno, Naoaki, additional, Tang, Walfred W.C., additional, Osada, Yasuyuki, additional, Hirao, Masao, additional, Yoshida, Fumika, additional, Sato, Hideyuki, additional, Nakauchi, Hiromitsu, additional, Hirabayashi, Masumi, additional, and Surani, M. Azim, additional

Published
2021
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5. DNMTs Play an Important Role in Maintaining the Pluripotency of Leukemia Inhibitory Factor-Dependent Embryonic Stem Cells

Author

Wu, Baojiang, primary, Li, Yunxia, additional, Li, Bojiang, additional, Zhang, Baojing, additional, Wang, Yanqiu, additional, Li, Lin, additional, Gao, Junpeng, additional, Fu, Yuting, additional, Li, Shudong, additional, Chen, Chen, additional, Surani, M. Azim, additional, Tang, Fuchou, additional, Li, Xihe, additional, and Bao, Siqin, additional

Published
2021
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6. Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage

Author

Zhu, Qifan, primary, Sang, Fei, additional, Withey, Sarah, additional, Tang, Walfred, additional, Dietmann, Sabine, additional, Klisch, Doris, additional, Ramos-Ibeas, Priscila, additional, Zhang, Haixin, additional, Requena, Cristina E., additional, Hajkova, Petra, additional, Loose, Matt, additional, Surani, M. Azim, additional, and Alberio, Ramiro, additional

Published
2021
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7. Activin A and BMP4 Signaling Expands Potency of Mouse Embryonic Stem Cells in Serum-Free Media

Author

Wu, Baojiang, primary, Li, Lin, additional, Li, Bojiang, additional, Gao, Junpeng, additional, Chen, Yanglin, additional, Wei, Mengyi, additional, Yang, Zhiqing, additional, Zhang, Baojing, additional, Li, Shudong, additional, Li, Kexin, additional, Wang, Changshan, additional, Surani, M. Azim, additional, Li, Xihe, additional, Tang, Fuchou, additional, and Bao, Siqin, additional

Published
2020
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8. Dynamic Heterogeneity and DNA Methylation in Embryonic Stem Cells

Author

Singer, Zakary S., Yong, John, Tischler, Julia, Hackett, Jamie A., Altinok, Alphan, Surani, M. Azim, Cai, Long, and Elowitz, Michael B.

Subjects
Resource, Gene Expression Profiling, Cell Biology, DNA Methylation, Time-Lapse Imaging, Epigenesis, Genetic, Mice, Animals, RNA, Messenger, Single-Cell Analysis, Transcriptome, Molecular Biology, Cells, Cultured, Embryonic Stem Cells, In Situ Hybridization, Fluorescence
Abstract

Summary Cell populations can be strikingly heterogeneous, composed of multiple cellular states, each exhibiting stochastic noise in its gene expression. A major challenge is to disentangle these two types of variability and to understand the dynamic processes and mechanisms that control them. Embryonic stem cells (ESCs) provide an ideal model system to address this issue because they exhibit heterogeneous and dynamic expression of functionally important regulatory factors. We analyzed gene expression in individual ESCs using single-molecule RNA-FISH and quantitative time-lapse movies. These data discriminated stochastic switching between two coherent (correlated) gene expression states and burst-like transcriptional noise. We further showed that the “2i” signaling pathway inhibitors modulate both types of variation. Finally, we found that DNA methylation plays a key role in maintaining these metastable states. Together, these results show how ESC gene expression states and dynamics arise from a combination of intrinsic noise, coherent cellular states, and epigenetic regulation., Graphical Abstract, Highlights • smFISH in ESCs reveals two transcriptional states and highly stochastic expression • Live-cell expression dynamics reveal the in situ transition rates between states • DNA methylation regulates state-switching dynamics • “2i” signaling inhibitors impact both gene expression noise and state transitions, Using single-molecule RNA-FISH and quantitative time-lapse movies, Singer et al. discriminate two primary sources of gene expression heterogeneity in embryonic stem cells: random switching between correlated gene expression states and burst-like transcriptional noise. Perturbing specific signaling pathways modulates both processes, and DNA methylation is key to maintaining these states.

Published
2014
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10. Dynamic Equilibrium and Heterogeneity of Mouse Pluripotent Stem Cells with Distinct Functional and Epigenetic States

Author

Hayashi, Katsuhiko, de Sousa Lopes, Susana M Chuva, Tang, Fuchou, Lao, Kaiqin, and Surani, M Azim

Subjects
Pluripotent Stem Cells, Chromosomal Proteins, Non-Histone, Cellular differentiation, Population, Cell Separation, Biology, Hydroxamic Acids, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Genetics, Animals, Homeostasis, Cell Lineage, Epigenetics, education, Induced pluripotent stem cell, Cells, Cultured, Embryonic Stem Cells, reproductive and urinary physiology, 030304 developmental biology, 0303 health sciences, education.field_of_study, urogenital system, Gene Expression Regulation, Developmental, Cell Differentiation, Cell Biology, DNA Methylation, Flow Cytometry, STEMCELL, Antigens, Differentiation, Embryonic stem cell, Molecular biology, Histone Deacetylase Inhibitors, Repressor Proteins, Epiblast, Blastocyst Inner Cell Mass, embryonic structures, DNA methylation, Molecular Medicine, biological phenomena, cell phenomena, and immunity, Stem cell, Germ Layers, 030217 neurology & neurosurgery
Abstract

Embryonic stem cells (ESC) are apparently homogeneous self-renewing cells, but Stella, a marker of preimplantation embryos and germ cells showed heterogeneous expression in ESCs. Here we show that the Stella-positive ESCs were like the inner cell mass (ICM) while the Stella-negative cells were like the epiblast cells, although these populations exhibited interchangeability that reflected an adherence to an inherent program. Thus, ESCs are in a metastable state since they shift between the ICM- and epiblast-like phenotypes, which provides an insight into their instability, plasticity and pluripotency. This equilibrium was skewed reversibly by environmental and epigenetic cues; for example, the absence of signals from feeder cells caused a shift towards an epiblast-like state while trichostatin A, an inhibitor of histone deactelylase, restored Stella-positive population. Consistently, the two populations showed different histone modifications but with hypomethylated DNA in stella locus, as well as functional differences when induced to undergo differentiation. The Stella-negative cells were apparently more like the post-implantation epiblast-derived stem cells (EpiSCs), except that the stella locus was hypermethylated and repressed in the latter, which denotes a robust boundary between ESCs and EpiSCs.

Published
2008

13. Trim28 Haploinsufficiency Triggers Bi-stable Epigenetic Obesity

Author

Dalgaard, Kevin, primary, Landgraf, Kathrin, additional, Heyne, Steffen, additional, Lempradl, Adelheid, additional, Longinotto, John, additional, Gossens, Klaus, additional, Ruf, Marius, additional, Orthofer, Michael, additional, Strogantsev, Ruslan, additional, Selvaraj, Madhan, additional, Lu, Tess Tsai-Hsiu, additional, Casas, Eduard, additional, Teperino, Raffaele, additional, Surani, M. Azim, additional, Zvetkova, Ilona, additional, Rimmington, Debra, additional, Tung, Y.C. Loraine, additional, Lam, Brian, additional, Larder, Rachel, additional, Yeo, Giles S.H., additional, O’Rahilly, Stephen, additional, Vavouri, Tanya, additional, Whitelaw, Emma, additional, Penninger, Josef M., additional, Jenuwein, Thomas, additional, Cheung, Ching-Lung, additional, Ferguson-Smith, Anne C., additional, Coll, Anthony P., additional, Körner, Antje, additional, and Pospisilik, J. Andrew, additional

Published
2016
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20. Rebuilding Pluripotency from Primordial Germ Cells

Author

Leitch, Harry G., primary, Nichols, Jennifer, additional, Humphreys, Peter, additional, Mulas, Carla, additional, Martello, Graziano, additional, Lee, Caroline, additional, Jones, Ken, additional, Surani, M. Azim, additional, and Smith, Austin, additional

Published
2013
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41. Esrrb Complementation Rescues Development of Nanog-Null Germ Cells

Author

Zhang, Man, Leitch, Harry G, Tang, Walfred WC, Festuccia, Nicola, Hall-Ponsele, Elisa, Nichols, Jennifer, Surani, M Azim, Smith, Austin, and Chambers, Ian

Subjects
urogenital system, competence, naive pluripotency, Cell Differentiation, Nanog Homeobox Protein, 3. Good health, Mice, Germ Cells, Receptors, Estrogen, transcription factors, embryonic structures, primordial germ cells, Animals, PGCLCs, biological phenomena, cell phenomena, and immunity, reproductive and urinary physiology
Abstract

The transcription factors (TFs) Nanog and Esrrb play important roles in embryonic stem cells (ESCs) and during primordial germ-cell (PGC) development. Esrrb is a positively regulated direct target of NANOG in ESCs that can substitute qualitatively for Nanog function in ESCs. Whether this functional substitution extends to the germline is unknown. Here, we show that germline deletion of Nanog reduces PGC numbers 5-fold at midgestation. Despite this quantitative depletion, Nanog-null PGCs can complete germline development in contrast to previous findings. PGC-like cell (PGCLC) differentiation of Nanog-null ESCs is also impaired, with Nanog-null PGCLCs showing decreased proliferation and increased apoptosis. However, induced expression of Esrrb restores PGCLC numbers as efficiently as Nanog. These effects are recapitulated in vivo: knockin of Esrrb to Nanog restores PGC numbers to wild-type levels and results in fertile adult mice. These findings demonstrate that Esrrb can replace Nanog function in germ cells.

42. Activin A and BMP4 Signaling Expands Potency of Mouse Embryonic Stem Cells in Serum-Free Media

Author

Wu, Baojiang, Li, Lin, Li, Bojiang, Gao, Junpeng, Chen, Yanglin, Wei, Mengyi, Yang, Zhiqing, Zhang, Baojing, Li, Shudong, Li, Kexin, Wang, Changshan, Surani, M Azim, Li, Xihe, Tang, Fuchou, and Bao, Siqin

Subjects
Pluripotent Stem Cells, Bone Morphogenetic Protein 4, chemically defined, Culture Media, Serum-Free, Genomic Instability, Epigenesis, Genetic, Mice, Animals, implantation, Cell Self Renewal, development, Protein Kinase Inhibitors, reproductive and urinary physiology, mouse, Mitogen-Activated Protein Kinase Kinases, epigenetics, urogenital system, blastocyst, Mouse Embryonic Stem Cells, embryonic stem cells, DNA Methylation, pluripotency, 3. Good health, genomic imprinting, Activins, Up-Regulation, hypermethylation, embryonic structures, biological phenomena, cell phenomena, and immunity, Signal Transduction
Abstract

Inhibitors of Mek1/2 and Gsk3β, known as 2i, and, together with leukemia inhibitory factor, enhance the derivation of embryonic stem cells (ESCs) and promote ground-state pluripotency (2i/L-ESCs). However, recent reports show that prolonged Mek1/2 suppression impairs developmental potential of ESCs, and is rescued by serum (S/L-ESCs). Here, we show that culturing ESCs in Activin A and BMP4, and in the absence of MEK1/2 inhibitor (ABC/L medium), establishes advanced stem cells derived from ESCs (esASCs). We demonstrate that esASCs contributed to germline lineages, full-term chimeras and generated esASC-derived mice by tetraploid complementation. We show that, in contrast to 2i/L-ESCs, esASCs display distinct molecular signatures and a stable hypermethylated epigenome, which is reversible and similar to serum-cultured ESCs. Importantly, we also derived novel ASCs (blASCs) from blastocysts in ABC/L medium. Our results provide insights into the derivation of novel ESCs with DNA hypermethylation from blastocysts in chemically defined medium.

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