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Your search keyword '"Stremersch, Stephan"' showing total 9 results
Start Over Author "Stremersch, Stephan" Publisher elsevier bv
9 results on '"Stremersch, Stephan"'

1. Laser-induced vapor nanobubbles for B16-F10 melanoma cell killing and intracellular delivery of chemotherapeutics

Author

Ramon, Jana, primary, Engelen, Yanou, additional, De Keersmaecker, Herlinde, additional, Goemaere, Ilia, additional, Punj, Deep, additional, Mejía Morales, Julián, additional, Bonte, Cédric, additional, Berx, Geert, additional, Hoste, Esther, additional, Stremersch, Stephan, additional, Lentacker, Ine, additional, De Smedt, Stefaan C., additional, Raemdonck, Koen, additional, and Braeckmans, Kevin, additional

Published
2024
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2. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Author

Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin, Afd Pharmaceutics, and Pharmaceutics

Subjects
Cell type, intracellular delivery, T cells, RM1-950, photoporation, MESENCHYMAL STEM-CELLS, Genome engineering, ACTIVATION, Cell therapy, DELIVERY, stem cells, Drug Discovery, Medicine and Health Sciences, Primary cell, gene editing, Chemistry, Mesenchymal stem cell, Biology and Life Sciences, Transfection, CAR, Cell biology, T-CELLS, Molecular Medicine, Therapeutics. Pharmacology, CRISPR-Cas9, Stem cell, SYSTEM, Intracellular
Abstract

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular delivery of Cas9 ribonucleoprotein (RNP) complexes in target cells poses a significant hurdle, especially in refractive primary cells. In the present work, vapor nanobubble (VNB) photoporation was explored for Cas9 RNP transfection in a variety of cell types. Proof of concept was first demonstrated in H1299-EGFP cells, before proceeding to hard-to-transfect stem cells and T cells. Gene knock-out levels over 80% and up to 60% were obtained for H1299 cells and mesenchymal stem cells, respectively. In these cell types, the unique possibility of VNB photoporation to knock out genes according to user-defined spatial patterns was demonstrated as well. Next, effective targeting of the programmed cell death 1 receptor and Wiskott-Aldrich syndrome gene in primary human T cells was demonstrated, reaching gene knock-out levels of 25% and 34%, respectively. With a throughput of >200,000 T cells per second, VNB photoporation is a scalable and versatile intracellular delivery method that holds great promise for CRISPR-Cas9-mediated ex vivo engineering of cell therapy products.

Published
2021

3. Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis

Author

Fraire, Juan C., primary, Houthaeve, Gaëlle, additional, Liu, Jing, additional, Raes, Laurens, additional, Vermeulen, Lotte, additional, Stremersch, Stephan, additional, Brans, Toon, additional, García-Díaz Barriga, Gerardo, additional, De Keulenaer, Sarah, additional, Van Nieuwerburgh, Filip, additional, De Rycke, Riet, additional, Vandesompele, Jo, additional, Mestdagh, Pieter, additional, Raemdonck, Koen, additional, De Vos, Winnok H., additional, De Smedt, Stefaan, additional, and Braeckmans, Kevin, additional

Published
2020
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7. Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori

Author

Santos, Rita S, primary, Dakwar, George R, additional, Xiong, Ranhua, additional, Forier, Katrien, additional, Remaut, Katrien, additional, Stremersch, Stephan, additional, Guimarães, Nuno, additional, Fontenete, Sílvia, additional, Wengel, Jesper, additional, Leite, Marina, additional, Figueiredo, Céu, additional, De Smedt, Stefaan C, additional, Braeckmans, Kevin, additional, and Azevedo, Nuno F, additional

Published
2015
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