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Salt stress is one of the major environmental stresses limiting plant growth and productivity. To adapt to salt stress, plants have developed various strategies to integrate exogenous salinity stress signals with endogenous developmental cues to optimize the balance of growth and stress responses. Accumulating evidence indicates that phytohormones, besides controlling plant growth and development under normal conditions, also mediate various environmental stresses, including salt stress, and thus regulate plant growth adaptation. In this review, we mainly discuss and summarize how plant hormones mediate salinity signals to regulate plant growth adaptation. We also highlight how, in response to salt stress, plants build a defense system by orchestrating the synthesis, signaling, and metabolism of various hormones via multiple crosstalks.

Alkali-salinity exerts severe osmotic, ionic and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test the functions of the Na2CO3-responsive proteins. A total of 104 and 102 Na2CO3-responsive proteins were identified in leaves and chloroplasts, respectively. In addition, 84 Na2CO3-responsive phosphoproteins were identified, including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts, which are crucial for the regulation of photosynthesis, ion transport, signal transduction and energy homeostasis. A full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, leading to enhanced Na2CO3 tolerance. All these results indicate that thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of photosystem (PS) II, PSI activity, and ROS homeostasis were altered in response to Na2CO3 stress, and they have improved our understanding of the Na2CO3-responsive mechanisms in halophytes.

Among the properties of homogeneity of incidence structures, flag-transitivity obviously is a particularly important and natural one. Originally, Buekenhout et al. reached a classification of flag-transitive Steiner 2-designs. Recently, Huber completely classified all flag-transitive Steiner t -designs with t ≤ 6 using the classification of the finite 2-transitive permutation groups. Hence the determination of all flag-transitive t -designs with λ ≥ 2 has remained of particular interest and has been known as a long-standing and still open problem.This article is a contribution to the study of the automorphism groups of 4-( v, k , 3) designs. Let S = ( P , B ) be a non-trivial 4- ( q + 1 , k , 3 ) design. If PSL (2, q ) acts flag-transitively on S , then S is a 4-(168,12,3) design and G B is conjugate to A 4 or Z 12 .

Alkali grass (Puccinellia tenuiflora), a monocotyledonous perennial halophyte species, is a good pasture with great nutritional value for livestocks. It can thrive under low temperature in the saline-alkali soil of Songnen plain in northeastern China. In the present study, the chilling-responsive mechanism in P. tenuiflora leaves was investigated using physiological and proteomic approaches. After treatment of 10°C for 10 and 20days, photosynthesis, biomass, contents of osmolytes and antioxidants, and activities of reactive oxygen species scavenging enzymes were analyzed in leaves of 20-day-old seedlings. Besides, 89 chilling-responsive proteins were revealed from proteomic analysis. All the results highlighted that the growth of seedlings was inhibited due to chilling-decreased enzymes in photosynthesis, carbohydrate metabolism, and energy supplying. The accumulation of osmolytes (i.e., proline, soluble sugar, and glycine betaine) and enhancement of ascorbate-glutathione cycle and glutathione peroxidase/glutathione S-transferase pathway in leaves could minimize oxidative damage of membrane and other molecules under the chilling conditions. In addition, protein synthesis and turnover in cytoplasm and chloroplast were altered to cope with the chilling stress. This study provides valuable information for understanding the chilling-responsive and cross-tolerant mechanisms in monocotyledonous halophyte plant species.

The article mainly deal with the block-transitive automorphism groups of 2 − ( v , k , 1 ) designs. Let G = P G L 2 ( q ) for q a power of an odd prime number. We prove that if G acts block-transitively on a non-trivial 2 − ( v , k , 1 ) design D then D is either a projective plane or 2 − ( 136 , 10 , 1 ) design.

Fern spore is a good single-cell model for studying the sophisticated molecular networks in asymmetric cell division, differentiation, and polar growth. Osmunda cinnamomea L. var. asiatica is one of the oldest fern species with typical separate-growing trophophyll and sporophyll. The chlorophyllous spores generated from sporophyll can germinate without dormancy. In this study, the spore ultrastructure, antioxidant enzyme activities, as well as protein and gene expression patterns were analyzed in the course of spore germination at five typical stages (i.e. mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Proteomic analysis revealed 113 differentially expressed proteins, which were mainly involved in photosynthesis, reserve mobilization, energy supplying, protein synthesis and turnover, reactive oxygen species scavenging, signaling, and cell structure modulation. The presence of multiple proteoforms of 25 differentially expressed proteins implies that post-translational modification may play important roles in spore germination. The dynamic patterns of proteins and their encoding genes exhibited specific characteristics in the processes of cell division and rhizoid tip growth, which include heterotrophic and autotrophic metabolisms, de novo protein synthesis and active protein turnover, reactive oxygen species and hormone (brassinosteroid and ethylene) signaling, and vesicle trafficking and cytoskeleton dynamic. In addition, the function skew of proteins in fern spores highlights the unique and common mechanisms when compared with evolutionarily divergent spermatophyte pollen. These findings provide an improved understanding of the typical single-celled asymmetric division and polar growth during fern spore germination.

The covalent attachment of O-linked β-N-acetylglucosamine (O-GlcNAc) to Ser/Thr residues of proteins acts as not only a posttranslational modification but also a nutritional sensor in nucleus and cytoplasm, which directly regulates the expression of genes and multiple crucial signal transduction pathways. Dynamic O-GlcNAcylation at Ser/Thr residues is catalyzed by two key enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase, which are responsible for addition and removal of the O-GlcNAc modification, respectively. O-GlcNAc modification plays important roles in cellular signaling in animals, especially in human diseases. Two orthologs of OGT in plants, SECRET AGENT and SPINDLY, have been reported to be involved in diverse plant processes. However, compared with the functional mechanisms revealed in animals, the consequences of protein O-GlcNAc modification in plants is largely unknown, and the relationship between O-GlcNAcylation and cellular processes needs to be explored. In this review, we summarized the recent advances on O-GlcNAc modification and its biological functions in animals and plants, and prospect of more special functions of O-GlcNAc will be revealed in plants.

Salinity is one of the major abiotic stresses that limits agricultural productivity worldwide. Plant roots function as the primary site of salinity perception. Salt responses in roots are essential for maintaining root functionality, as well as for transmitting the salt signal to shoot for proper salt response and adaptation in the entire plant. Therefore, a thorough understanding of signaling and metabolic mechanisms of salt response in roots is critical for improving plant salt tolerance. Current proteomic studies have provided salt-responsive expression patterns of 905 proteins in 14 plant species. Through integrative analysis of salt-responsive proteins and previous physiological and molecular findings, this review summarizes current understanding of salt responses in roots and highlights proteomic findings on the molecular mechanisms in the fine-tuned salt-responsive networks. At the proteome level, the following processes become dominant in root salt response: (i) salt signal perception and transduction; (ii) detoxification of reactive oxygen species (ROS); (iii) salt uptake/exclusion and compartmentalization; (iv) protein translation and/or turnover dynamics; (v) cytoskeleton/cell wall dynamics; (vi) carbohydrate and energy metabolism; and (vii) other salt-responsive metabolisms. These processes work together to gain cellular homeostasis in roots and determine the overall phenotype of plant growth and development under salt stress.

Guard cells are highly specialized cells that form tiny pores called stomata on the leaf surface. The opening and closing of stomata control leaf gas exchange and water transpiration as well as allow plants to quickly respond and adjust to new environmental conditions. Mesophyll cells are specialized for photosynthesis. Despite the phenotypic and obvious functional differences between the two types of cells, the full protein components and their functions have not been explored but are addressed here through a global comparative proteomics analysis of purified guard cells and mesophyll cells. With the use of isobaric tags for relative and absolute quantification (iTRAQ) tagging and two-dimensional liquid chromatography mass spectrometry, we identified 1458 non-redundant proteins in both guard cells and mesophyll cells of Brassica napus leaves. Based on stringent statistical criteria, a total of 427 proteins were quantified, and 74 proteins were found to be enriched in guard cells. Proteins involved in energy (respiration), transport, transcription (nucleosome), cell structure, and signaling are preferentially expressed in guard cells. We observed several well characterized guard cell proteins. By contrast, proteins involved in photosynthesis, starch synthesis, disease/defense/stress, and other metabolisms are preferentially represented in mesophyll cells. Of the identified proteins, 110 have corresponding microarray data obtained from Arabidopsis guard cells and mesophyll cells. About 72% of these proteins follow the same trend of expression at the transcript and protein levels. For the rest of proteins, the correlation between proteomics data and the microarray data is poor. This highlights the importance of quantitative profiling at the protein level. Collectively this work represents the most extensive proteomic description of B. napus guard cells and has improved our knowledge of the functional specification of guard cells and mesophyll cells.

Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. Because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants of Arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. Here we describe a method for obtaining a large quantity of in vitro germinating rice pollen for proteomics study. Two-dimensional electrophoresis of approximately 2300 protein spots revealed 186 that were differentially expressed in mature and germinated pollen. Most showed a changed level of expression, and only 66 appeared to be specific to developmental stages. Furthermore 160 differentially expressed protein spots were identified on mass spectrometry to match 120 diverse protein species. These proteins involve different cellular and metabolic processes with obvious functional skew toward wall metabolism, protein synthesis and degradation, cytoskeleton dynamics, and carbohydrate/energy metabolism. Wall metabolism-related proteins are prominently featured in the differentially expressed proteins and the pollen proteome as compared with rice sporophytic proteomes. Our study also revealed multiple isoforms and differential expression patterns between isoforms of a protein. These results provide novel insights into pollen function specialization.

Salidroside is the active ingredient in the roots of a Chinese medicine herb, Rhodiola sachalinensis A. Bor. Salidroside content in the Rh. sachalinensis differs greatly in different natural habitats. The effects of soil nutrient factors on the salidroside production in the root of Rh. sachalinensis were for the first time investigated in Chang Bai Mountain, PR China. The results showed that rich organic matter, low pH and high levels of exchangeable nitrogen and total nitrogen in soil were most essential to guarantee the high productivity of salidroside. The salidroside yield reached higher than 0.7% of root dry weight when the soil pH value was about 4.5, the soil organic matter content was higher than 17% or soil exchangeable nitrogen and total nitrogen contents were higher than 34 mg/100 mg and 0.6% of soil dry weight, respectively. However, soil bioavailable phosphorus content higher than 5 ppm and exchangeable potassium content higher than 180 ppm resulted in a low salidroside yield, only about 0.2% dry weight. This investigation will guide the soil management on how to select soil and how to fertilize the soil for the cultivation of Rh. sachalinensis to improve the salidroside productivity.