Search

Your search keyword '"Scudiero, Rosaria"' showing total 60 results
Start Over Author "Scudiero, Rosaria" Publisher elsevier bv
60 results on '"Scudiero, Rosaria"'

2. Exploring the molecular and toxicological mechanism associated with interactions between heavy metals and the reproductive system of Mytilus galloprovincialis

Author

Marinaro, Carmela, primary, Lettieri, Gennaro, additional, Chianese, Teresa, additional, Bianchi, Anna Rita, additional, Zarrelli, Armando, additional, Palatucci, Domenico, additional, Scudiero, Rosaria, additional, Rosati, Luigi, additional, De Maio, Anna, additional, and Piscopo, Marina, additional

Published
2024
Full Text
View/download PDF

47. Aluminium exposure leads to neurodegeneration and alters the expression of marker genes involved to parkinsonism in zebrafish brain

Author

Teresa Capriello, Gianluca Di Meglio, Anna De Maio, Rosaria Scudiero, Anna Rita Bianchi, Marco Trifuoggi, Maria Toscanesi, Antonella Giarra, Ida Ferrandino, Capriello, Teresa, Di Meglio, Gianluca, DE MAIO, Anna, Scudiero, Rosaria, Bianchi, ANNA RITA, Trifuoggi, Marco, Toscanesi, Maria, Giarra, Antonella, and Ferrandino, Ida

Subjects
Mammals, Environmental Engineering, Health, Toxicology and Mutagenesis, Public Health, Environmental and Occupational Health, Brain, Neurodegenerative Diseases, Parkinson Disease, General Medicine, General Chemistry, Poly(ADP-ribose) Polymerase Inhibitors, Pollution, Brain, Gene expression, Glial fibrillary acidic protein, PARP, Histology, Myelin, Glial Fibrillary Acidic Protein, Animals, Environmental Chemistry, Neurotoxicity Syndromes, Poly(ADP-ribose) Polymerases, Biomarkers, Zebrafish, Aluminum
Abstract

Aluminium, despite being extremely widespread in the world, is a non-essential metal to human metabolism. This metal is known to have toxic effects on a variety of organs including the brain and is considered an etiological factor in neurodegenerative diseases. However, the molecular mechanisms by which aluminium exerts neurotoxic effects are not yet completely understood. Zebrafish is an animal model also used to study neurodegenerative diseases since the overall anatomical organization of the central nervous system is relatively conserved and similar to mammals. Adult zebrafish were exposed to 11 mg/L of Al for 10, 15, and 20 days and the neurotoxic effects of aluminium were analysed by histological, biochemical, and molecular evaluations. Histological stainings allowed to evaluation of the morphology of the brain parenchyma, the alteration of myelin and the activation of neurodegenerative processes. The expression of the Glial Fibrillary Acidic Protein, a marker of glial cells, was evaluated to observe the quantitative alteration of this important protein for the nervous system. In addition, the poly(ADP-ribose) polymerase activity was measured to verify a possible oxidative DNA damage caused by exposure to this metal. Finally, the evaluation of the markers involved in Parkinsonism was assessed by Real-Time PCR to better understand the role of aluminium in the regulation of genes related to Parkinson's neurodegenerative disease. Data showed that aluminium significantly affected the histology of cerebral tissue especially in the first periods of exposure, 10 and 15 days. This trend was also followed by the expression of GFAP. At longer exposure times, there was an improvement/stabilization of the overall neurological conditions and decrease in PARP activity. In addition, aluminium is involved in the deregulation of the expression of genes closely related to Parkinsonism. Overall, the data confirm the neurotoxicity induced by aluminium and shed a light on its involvement in neurodegenerative processes.

Published
2022
Full Text
View/download PDF

48. Role of estrogen receptors, P450 aromatase, PCNA and p53 in high-fat-induced impairment of spermatogenesis in rats

Author

Mariailaria Verderame, Vincenzo Migliaccio, Rosaria Scudiero, Verderame, Mariailaria, Migliaccio, Vincenzo, and Scudiero, Rosaria

Subjects
Male, 0301 basic medicine, medicine.medical_specialty, Estrogen receptor, Adipose tissue, Estrogen receptors, spermatogenesis, estrogen receptors, hyperlipidic diet, aromatase, PCNA, p53, rats, mammals, In situ hybridization, Wistar rat, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Aromatase, 0302 clinical medicine, Western blot, Proliferating Cell Nuclear Antigen, Internal medicine, Testis, medicine, Animals, PCNA, Rats, Wistar, Spermatogenesis, P53, High-fat diet, 030219 obstetrics & reproductive medicine, General Immunology and Microbiology, biology, medicine.diagnostic_test, Estrogen Receptor alpha, Estrogens, General Medicine, Spermatids, Epithelium, Rats, Proliferating cell nuclear antigen, Seminiferous Epithelium, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Receptors, Estrogen, biology.protein, Tumor Suppressor Protein p53, General Agricultural and Biological Sciences
Abstract

Obesity and overweight are frequently associated with male subfertility. To address new findings on the players involved in the obesity-induced impairment of spermatogenesis, we used a high-fat diet-induced overweight-rat model. Following four weeks of high-fat diet, the organization of seminiferous epithelium was affected, and tubules lumen showed immature/degenerated cells, typical signs of hormonal imbalance and testicular damage. Real-time PCR analysis allowed us to detect increased levels of ERα and decreased levels of aromatase CYP19 transcripts in testis, suggesting an increase in circulating estrogens derived from the accumulating adipose tissue rather than the induction of testicular estrogen synthesis. Moreover, in situ hybridization analysis showed an increased susceptibility towards estrogens in testis from high-fat fed rats, being ERs expressed not only in spermatogonia, as in control testis, but also in spermatids. Western blot and immunohistochemical analyses revealed an increase in the amount of p53 and PCNA, together with a change in their immunodetection, being the proteins localised on germ cells at different stages of maturation. Differences in p53 and PCNA expression may give evidence and be part of a cellular response to stress conditions and damage caused by the excessive intake of saturated fatty acids.

Published
2018
Full Text
View/download PDF

49. Retinoblastoma binding protein 6 and crystallin lambda 1 are cadmium-responsive genes in zebrafish embryos and adults retinae

Author

Palma Simoniello, Chiara Maria Motta, Maria Esposito, Rosaria Scudiero, Scudiero, Rosaria, Esposito, Mg, Simoniello, P, and Motta, CHIARA MARIA

Subjects
Male, Genetics and Molecular Biology (all), inorganic chemicals, 0301 basic medicine, Embryo, Nonmammalian, animal structures, Immunology and Microbiology (all), Adult zebrafish retina, Danio, chemistry.chemical_element, Zebrafish embryos, 010501 environmental sciences, Protein degradation, Biochemistry, 01 natural sciences, Retina, General Biochemistry, Genetics and Molecular Biology, Lens protein, 03 medical and health sciences, Crystallin, Animals, Cadmium toxicology, gene expression, embryo development, retina, zebrafish, Zebrafish, mRNA differential display, 0105 earth and related environmental sciences, Retinoblastoma Binding Proteins, Cadmium, General Immunology and Microbiology, biology, Medicine (all), Binding protein, General Medicine, biology.organism_classification, Crystallins, Molecular biology, 030104 developmental biology, Agricultural and Biological Sciences (all), chemistry, Female, Gene expression, In situ hybridization, Biochemistry, Genetics and Molecular Biology (all), General Agricultural and Biological Sciences
Abstract

Nonessential metal cadmium is widely used and released in the environment, causing cell toxicity and posing a severe threat to wildlife. Zebrafish (Danio rerio) is one of the most commonly used animals in the investigation of environmental cadmium toxicity in vertebrates. In this study, we identified two cadmium-responsive genes, RBBP6 and CRYL1, in the early phases of zebrafish development, at the gastrula stage. The retinoblastoma binding protein 6 is associated with increased protein degradation and cell proliferation; crystallin-lambda 1 is a lens protein with redox activity. In situ hybridization analysis performed on adult zebrafish exposed to 1.5-40 μM cadmium for 30 days confirmed the ability of cadmium to up-regulate the expression of both genes in retinal cells in a dose-dependent manner. The over-expression was transient, being switched off when cadmium was removed. The involvement of RBBP6 and CRYL1 in the onset of cadmium-induced morphological alterations in adult zebrafish retina is discussed.

Published
2017
Full Text
View/download PDF

50. Identification and expression of an atypical isoform of metallothionein in the African clawed frog Xenopus laevis

Author

Rosaria Scudiero, Rosa Carotenuto, Margherita Tussellino, Scudiero, Rosaria, Tussellino, Margherita, and Carotenuto, Rosa

Subjects
Gene isoform, Aging, African clawed frog, DNA, Complementary, Molecular Sequence Data, Xenopus laevi, Xenopus, Real-Time Polymerase Chain Reaction, Real Time, General Biochemistry, Genetics and Molecular Biology, Xenopus laevis, Animals, Protein Isoforms, Coding region, Metallothionein, Amino Acid Sequence, Cysteine, Cloning, Molecular, Gene, Western clawed frog, Phylogeny, Silurana, Genetics, Base Sequence, General Immunology and Microbiology, biology, Computational Biology, General Medicine, biology.organism_classification, Biological Evolution, Molecular biology, Gene Expression Regulation, RNA, Gene expression, General Agricultural and Biological Sciences
Abstract

Exploiting the annotation of the western clawed frog Silurana tropicalis genome, we identified a new metallothionein (MT) gene, exhibiting all the features to be considered an active gene, but with an atypical coding region, showing only 17 cysteine residues instead of the canonical 20 cysteines of vertebrate metallothioneins and two anomalous cysteine triplets. However, the presence of a gene in the genome does not ensure its effective expression. By using conventional and Real-Time PCR analyses, we demonstrated that this atypical MT is constitutively expressed throughout the life cycle of the African clawed frog Xenopus laevis; moreover, this gene is highly expressed in the adult liver, the major site of MT expression and synthesis in vertebrates. To our knowledge, the X. laevis MT described in this paper is the first sequence of a vertebrate MT showing only 17 cysteine residues, arranged in two Cys–Cys–Cys motifs. Phylogenetic analyses also demonstrated that the atypical X. laevis MT merges in the anuran clade, but is the most derived sequence among tetrapods MTs. Finally, Tajima's Relative Rate Test suggested a different evolutionary rate between the canonical X. laevis MT and this novel isoform.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results