1. Micropropagation in wild pear (Pyrus syricca)
- Author
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M. M. Ajlouni, S. Aljanabi, Mohamad A. Shatnawi, A. Jaradat, and Rida A. Shibli
- Subjects
Butyric acid ,Indole test ,Horticulture ,chemistry.chemical_compound ,Murashige and Skoog medium ,chemistry ,Callus formation ,Cytokinin ,Botany ,Shoot ,Zeatin ,Explant culture - Abstract
Some factors which affect the in vitro propagation of wild pear ( Pyrus syrica ) were studied. Successful shoot proliferation was obtained on Murashige and Skoog medium supplemented with 1.5–2.0 mg 1 −1 benzyl adenine. Shoot height decreased to 1.8–1.6 cm and 1.3 cm with benzyl adenine (1.5–2.0 mg 1 −1 ) and zeatin (1.0–2.0 mg 1 −1 ), respectively. Benzyl adenine (at 2.0 mg 1 −1 ) and zeatin (at 1.5–2.0 mg 1 −1 ) enhanced callus formation. Indole butyric acid, indole acetic acid and naphthalene acetic acid induced in vitro rooting and a maximum of 72% rooting was achieved at 3.0 mg 1 −1 indole acetic acid. Neither activated charcoal nor polyvinylpyrrolidone influenced in vitro rooting. Ex vitro rooting was unsuccessful when treating shoot microcuttings with 0.0, 5.0, 10.0, or 15.0 mg 1 −1 indole butyric acid, indole acetic acid, or naphthalene acetic acid for l h and grown under intermittent water mist. An average of 95% survival was achieved when rooted microshoots were acclimatized under glass or plastic covers using intermittent mist in the greenhouse.
- Published
- 1997
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