1. Identification of novel phasiRNAs loci on long non-coding RNAs in Arabidopsis thaliana
- Author
-
Lan Yu, Yijun Meng, Zhihong Yang, Rongkai Guo, Chaogang Shao, Yeqin Jiang, and Xinghuo Ye
- Subjects
0106 biological sciences ,Small RNA ,Arabidopsis ,Computational biology ,01 natural sciences ,Gossypium hirsutum ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Genetics ,Aromatic amino acids ,RNA, Small Interfering ,030304 developmental biology ,Regulation of gene expression ,Gossypium ,0303 health sciences ,Oryza sativa ,biology ,Oryza ,biology.organism_classification ,Plant development ,chemistry ,Genetic Loci ,RNA, Plant ,RNA, Long Noncoding ,Soybeans ,010606 plant biology & botany - Abstract
Long non-coding RNAs (lncRNAs) are the "dark matters"involved in gene regulation with complex mechanisms. However, the functions of most lncRNAs remain to be determined. Our previous work revealed a massive number of degradome-supported cleavage signatures on Arabidopsis lncRNAs. Some of them have been confirmed associated with miRNAs-like sRNAs production, while others without long stem structure remain unexplored. A systematical search for phasiRNAs generating ability of these lncRNAs was conducted. Eight novel small RNA triggered lncRNA-phasiRNA pathways were discovered and three of them were found to be conserved in Arabidopsis, Oryza sativa, Glycine max and Gossypium hirsutum. Besides, Five novel ta-siRNAs derived from these lncRNAs were further identified to be involved in the regulation of plant development, stress responses and aromatic amino acids synthesis. These results substantially expanded the gene regulation mechanisms of lncRNAs.
- Published
- 2019