1. Mutagenesis resulting from depurination is an SOS process
- Author
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Barry W. Glickman, Roeland M. Schaaper, and Lawrence A. Loeb
- Subjects
endocrine system ,DNA Repair ,Genes, Viral ,Ultraviolet Rays ,Health, Toxicology and Mutagenesis ,Transfection ,Bacteriophage ,chemistry.chemical_compound ,Genetics ,AP site ,Molecular Biology ,Recombination, Genetic ,biology ,fungi ,Mutagenesis ,food and beverages ,DNA ,Spheroplast ,biology.organism_classification ,Molecular biology ,chemistry ,Purines ,Mutation ,bacteria ,Depurination ,Bacteriophage phi X 174 ,Bacteria - Abstract
When bacteriophage φX174 am3 DNA depurinated in vitro is transferted into E. coli spherplasts prepared from bacteria previously exposed to UV light, a strong mutagenic response is observed. This mutagenic response does not occur in spheroplasts derived from pre-irradiated bacteria carrying defective recF, recF or umuC genes. These findings indicate that mutagenesis at apurinic sites is an SOS-dependent process. The mutagenic response is not dependent on the multiplicity of transfection. This suggests that mutagenesis is not mediated by recombination.
- Published
- 1982
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