Search

Your search keyword '"Ries, Jonas"' showing total 40 results
Start Over Author "Ries, Jonas" Publisher elsevier bv
40 results on '"Ries, Jonas"'

3. The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation

Author

Cosentino, Katia, primary, Hertlein, Vanessa, additional, Jenner, Andreas, additional, Dellmann, Timo, additional, Gojkovic, Milos, additional, Peña-Blanco, Aida, additional, Dadsena, Shashank, additional, Wajngarten, Noel, additional, Danial, John S.H., additional, Thevathasan, Jervis Vermal, additional, Mund, Markus, additional, Ries, Jonas, additional, and Garcia-Saez, Ana J., additional

Published
2022
Full Text
View/download PDF

22. A Paired RNAi and RabGAP Overexpression Screen Identifies Rab11 as a Regulator of β-Amyloid Production

Author

Udayar, Vinod, primary, Buggia-Prévot, Virginie, additional, Guerreiro, Rita L., additional, Siegel, Gabriele, additional, Rambabu, Naresh, additional, Soohoo, Amanda L., additional, Ponnusamy, Moorthi, additional, Siegenthaler, Barbara, additional, Bali, Jitin, additional, Simons, Mikael, additional, Ries, Jonas, additional, Puthenveedu, Manojkumar A., additional, Hardy, John, additional, Thinakaran, Gopal, additional, Rajendran, Lawrence, additional, Guerreiro, Rita, additional, Brás, José, additional, Sassi, Celeste, additional, Gibbs, J. Raphael, additional, Hernandez, Dena, additional, Lupton, Michelle K., additional, Brown, Kristelle, additional, Morgan, Kevin, additional, Powell, John, additional, and Singleton, Andrew, additional

Published
2013
Full Text
View/download PDF

39. Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation

Author

Mund, Markus, Van Der Beek, Johannes Albertus, Deschamps, Joran, Dmitrieff, Serge, Hoess, Philipp, Monster, Jooske Louise, Picco, Andrea, Nédélec, François, Kaksonen, Marko, and Ries, Jonas

Subjects
Saccharomyces cerevisiae Proteins, Protein Conformation, Secretory Vesicles, Cell Membrane, WASP, Saccharomyces cerevisiae, Models, Theoretical, Actins, Endocytosis, 3. Good health, Wiskott-Aldrich Syndrome Protein Family, superresolution microscopy, Actin Cytoskeleton, Microscopy, Fluorescence, clathrin, Brownian dynamics simulations, SMLM, actin, single-molecule localization microscopy, high-throughput
Abstract

Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision and efficiency is not understood. Here, we developed high-throughput superresolution microscopy to reconstruct the nanoscale structural organization of 23 endocytic proteins from over 100,000 endocytic sites in yeast. We found that proteins assemble by radially ordered recruitment according to function. WASP family proteins form a circular nanoscale template on the membrane to spatially control actin nucleation during vesicle formation. Mathematical modeling of actin polymerization showed that this WASP nano-template optimizes force generation for membrane invagination and substantially increases the efficiency of endocytosis. Such nanoscale pre-patterning of actin nucleation may represent a general design principle for directional force generation in membrane remodeling processes such as during cell migration and division.

40. Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation

Author

François Nédélec, Johannes Albertus van der Beek, Serge Dmitrieff, Markus Mund, Jooske Louise Monster, Jonas Ries, Marko Kaksonen, Philipp Hoess, Joran Deschamps, Andrea Picco, Mund, Markus [0000-0001-6449-743X], Deschamps, Joran [0000-0001-8462-2883], Dmitrieff, Serge [0000-0002-1362-5670], Hoess, Philipp [0000-0003-2390-2245], Picco, Andrea [0000-0003-2548-9183], Ries, Jonas [0000-0002-6640-9250], Apollo - University of Cambridge Repository, and Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Saccharomyces cerevisiae Proteins, Protein Conformation, [SDV]Life Sciences [q-bio], Endocytic cycle, WASP, Saccharomyces cerevisiae, Biology, Endocytosis, Clathrin, Article, General Biochemistry, Genetics and Molecular Biology, superresolution microscopy, 03 medical and health sciences, clathrin, Brownian dynamics simulations, SMLM, ComputingMilieux_MISCELLANEOUS, high-throughput, Actin, Membrane invagination, Actin nucleation, Secretory Vesicles, Cell Membrane, Models, Theoretical, Actin cytoskeleton, Actins, Wiskott-Aldrich Syndrome Protein Family, 3. Good health, Actin Cytoskeleton, 030104 developmental biology, Endocytic vesicle, Microscopy, Fluorescence, Biophysics, biology.protein, actin, single-molecule localization microscopy
Abstract

Summary Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision and efficiency is not understood. Here, we developed high-throughput superresolution microscopy to reconstruct the nanoscale structural organization of 23 endocytic proteins from over 100,000 endocytic sites in yeast. We found that proteins assemble by radially ordered recruitment according to function. WASP family proteins form a circular nanoscale template on the membrane to spatially control actin nucleation during vesicle formation. Mathematical modeling of actin polymerization showed that this WASP nano-template optimizes force generation for membrane invagination and substantially increases the efficiency of endocytosis. Such nanoscale pre-patterning of actin nucleation may represent a general design principle for directional force generation in membrane remodeling processes such as during cell migration and division., Graphical Abstract, Highlights • High-throughput superresolution imaging of 23 proteins at thousands of endocytic sites • Endocytic proteins organize into nanoscale radial zones according to their function • A circular WASP nano-template patterns actin filament nucleation • The WASP nano-template is crucial for high efficiency of endocytosis, Superresolution analysis of endocytic vesicle assembly identifies a patterned actin template that orchestrates protein recruitment.

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results