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Your search keyword '"Restrepo S"' showing total 12 results
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12 results on '"Restrepo S"'

5. Highly efficient transformation system for Malassezia furfur and Malassezia pachydermatis using Agrobacterium tumefaciens-mediated transformation

Author

Celis, A.M., Vos, Aurin, Triana, S., Medina, C.A., Escobar Salazar, Natalia, Restrepo, S., Wosten, Han, de Cock, Hans, Sub Molecular Microbiology, Molecular Microbiology, Sub Molecular Microbiology, and Molecular Microbiology

Subjects
0301 basic medicine, green fluorescent protein, polymerase chain reaction, Agaricus, Agrobacterium, fluorescence microscopy, Polymerase Chain Reaction, hygromycin B, chemistry.chemical_compound, Malassezia pachydermatis, biology, integumentary system, Malassezia furfur, Agrobacterium tumefaciens, Agaricus bisporus, DNA vector, Phosphotransferases (Alcohol Group Acceptor), T-DNA, Malassezia spp, Malassezia, genetic marker, coculture, DNA, Bacterial, Microbiology (medical), 030106 microbiology, Genetic Vectors, Green Fluorescent Proteins, GFP, Microbiology, Hygromycin, Transformation, 03 medical and health sciences, Transformation, Genetic, Dermatomycoses, Humans, human, phosphotransferase, Molecular Biology, Cryptococcus neoformans, nonhuman, biology.organism_classification, Coculture Techniques, Transformation (genetics), 030104 developmental biology, chemistry, Microscopy, Fluorescence, genetic transformation, Hygromycin B, Transformation efficiency
Abstract

Malassezia spp. are part of the normal human and animal mycobiota but are also associated with a variety of dermatological diseases. The absence of a transformation system hampered studies to reveal mechanisms underlying the switch from the non-pathogenic to pathogenic life style. Here we describe, a highly efficient Agrobacterium-mediated genetic transformation system for Malassezia furfur and M. pachydermatis. A binary T-DNA vector with the hygromycin B phosphotransferase (hpt) selection marker and the green fluorescent protein gene (gfp) was introduced in M. furfur and M. pachydermatis by combining the transformation protocols of Agaricus bisporus and Cryptococcus neoformans. Optimal temperature and co-cultivation time for transformation were 5 and 7 days at 19 °C and 24 °C, respectively. Transformation efficiency was 0.75–1.5% for M. furfur and 0.6–7.5% for M. pachydermatis. Integration of the hpt resistance cassette and gfp was verified using PCR and fluorescence microscopy, respectively. The T-DNA was mitotically stable in approximately 80% of the transformants after 10 times sub-culturing in the absence of hygromycin. Improving transformation protocols contribute to study the biology and pathophysiology of Malassezia.

Published
2017
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6. Modeling and simulation in tribology across scales: An overview

Author

Vakis, A.I., primary, Yastrebov, V.A., additional, Scheibert, J., additional, Nicola, L., additional, Dini, D., additional, Minfray, C., additional, Almqvist, A., additional, Paggi, M., additional, Lee, S., additional, Limbert, G., additional, Molinari, J.F., additional, Anciaux, G., additional, Aghababaei, R., additional, Echeverri Restrepo, S., additional, Papangelo, A., additional, Cammarata, A., additional, Nicolini, P., additional, Putignano, C., additional, Carbone, G., additional, Stupkiewicz, S., additional, Lengiewicz, J., additional, Costagliola, G., additional, Bosia, F., additional, Guarino, R., additional, Pugno, N.M., additional, Müser, M.H., additional, and Ciavarella, M., additional

Published
2018
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