1. Capturing prokaryotic dark matter genomes
- Author
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Pierre Peyret, Nicolas Parisot, Eric Peyretaillade, Réjane Beugnot, Clémence Defois, Delphine Boucher, Corinne Petit-Biderre, Céline Ribière, Cyrielle Gasc, Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), Université d'Auvergne - Clermont-Ferrand I (UdA), Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Auvergne Regional Council, European Regional Development Fund, program Investissements d'Avenir AMI VALTEX, French 'Ministere de l'Enseignement Superieur et de la Recherche', French 'Direction Generale de l'Armement' (DGA), and Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA)
- Subjects
Single-cell genomics ,[SDV]Life Sciences [q-bio] ,Dark matter ,Biology ,Microbiology ,Genome ,Cellular life ,03 medical and health sciences ,Genome, Archaeal ,Ecosystem ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,Complete genome reconstruction ,030304 developmental biology ,0303 health sciences ,Bacteria ,030306 microbiology ,Ecology ,High-Throughput Nucleotide Sequencing ,General Medicine ,15. Life on land ,Archaea ,Prokaryotic Cells ,Metagenomics ,Evolutionary biology ,Single-Cell Analysis ,Genome, Bacterial ,Function (biology) - Abstract
Prokaryotes are the most diverse and abundant cellular life forms on Earth. Most of them, identified by indirect molecular approaches, belong to microbial dark matter. The advent of metagenomic and single-cell genomic approaches has highlighted the metabolic capabilities of numerous members of this dark matter through genome reconstruction. Thus, linking functions back to the species has revolutionized our understanding of how ecosystem function is sustained by the microbial world. This review will present discoveries acquired through the illumination of prokaryotic dark matter genomes by these innovative approaches.
- Published
- 2015