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Sortilin-related VPS domain containing receptor 1 (SORCS1), is located on chromosome 10q23.3, a chromosomal region of interest in Alzheimer's disease (AD) defined by many genome-wide and chromosome10-specific studies. Recently, three intronic variants (rs12571141, rs17277986 and rs6584777) within SORCS1 were reported to be associated with AD in Caucasian. In order to assess the involvement of the SORCS1 polymorphisms in the progression of late-onset AD (LOAD), we conducted an independent replication study in 1198 unrelated Northern Han Chinese subjects comprising 598 LOAD patients and 600 healthy controls matched for gender and age. The results revealed no significant differences in the distributions of genotype or allele between LOAD and control groups in the total sample. However, when these data were stratified by the Apolipoprotein E (APOE) e4 status, we observed significant differences in the genotypes and allele frequencies (rs12571141: P = 0.001, rs17277986: P = 0.005, rs6584777: P = 0.023) in APOE e4 allele carriers. Moreover, the association was further demonstrated in logistic regression analysis (rs12571141: P = 0.002, OR = 0.424; rs17277986: P = 0.004, OR = 0.447; rs6584777: P = 0.019, OR = 0.523) and haplotype analysis (GCC: P = 0.002, ATT: P = 0.002, ACC: P = 0.025) in this subset. Our data suggested that SORCS1 was in interaction with APOE in the development of LOAD in a Northern Han Chinese population.

Using oleic acid as stabilizer, the monodisperse Co–Ni–Cu amorphous alloys nanoparticles were synthesized by the reduction of CuSO4·5H2O, NiCl2·6H2O and CoCl2·6H2O with KBH4 in positive microemulsion (hexane/sodium oleate/water) system. All results show that the as-synthesized spherical particles are air-stable amorphous alloy nanoparticles in room temperature with the size of 4–7 nm in narrow deviation, and are coated by oleic acid. These amorphous alloy nanoparticles have unique soft magnetic property with small coercivity (Hc) of 7.94 Oe for CuNi, 37.65 Oe for CoCu, 42.17 Oe for CoNiCu and 65.09 Oe for CoNi, respectively. After annealed at 923 K for 0.5 h, the Hc of CuNi, CoCu, CoNiCu and CoNi increased significantly to 46.7, 370.04, 337.4 and 305.5 Oe, respectively. It corresponds to the crystallization of amorphous alloy nanoparticles.

Monoclonal antibodies to epithelial membrane antigen and common acute lymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were then used to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 × 10 6 epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment.