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101 results on '"Polakova A"'

4. Prediction of Sudden Cardiac Arrest After Alcohol Septal Ablation for Hypertrophic Obstructive Cardiomyopathy: ASA-SCARRE Risk Score

Author

Josef Veselka, Max Liebregts, Robert Cooper, Lothar Faber, Jaroslav Januska, Maksim Kashtanov, Klara Hulikova Tesarkova, Peter Riis Hansen, Hubert Seggewiss, Eugene Shloydo, Kirill Popov, Eva Hansvenclova, Eva Polakova, Jurriën ten Berg, Rodney Hilton Stables, Jiri Jarkovsky, and Jiri Bonaventura

Subjects
Male, Adult, Ablation Techniques, Ethanol, Middle Aged, Cardiomyopathy, Hypertrophic, Death, Sudden, Cardiac, Treatment Outcome, Risk Factors, Humans, Female, Cardiac Surgical Procedures, Cardiology and Cardiovascular Medicine, Aged
Abstract

This study aimed to derive a new score, the Alcohol Septal Ablation-Sudden Cardiac ARREst (ASA-SCARRE) risk score, that can be easily used to evaluate the risk of sudden cardiac arrest events (sudden cardiac death, resuscitation, or appropriate implantable cardioverter-defibrillator discharge) after alcohol septal ablation (ASA) in patients with hypertrophic obstructive cardiomyopathy. We analyzed 1,834 patients from the Euro-ASA registry (49% men, mean age 57 ± 14 years) who were followed up for 5.0 ± 4.3 years (9,202 patient-years) after ASA. A total of 65 patients (3.5%) experienced sudden cardiac arrest events, translating to 0.72 events per 100 patient-years. The independent predictors of sudden cardiac arrest events were septum thickness before ASA (hazard ratio 1.09 per 1 mm, 95% confidence interval 1.04 to 1.14, p0.001) and left ventricular outflow tract (LVOT) gradient at the last clinical checkup (hazard ratio 1.01 per 1 mm Hg, 95% confidence interval 1.01 to 1.02, p = 0.002). The following ASA-SCARRE risk scores were derived and independently predicted long-term risk of sudden cardiac arrest events: "0" for both LVOT gradient30 mmHg and baseline septum thickness20 mm; "1" for LVOT gradient ≥30 mm Hg or baseline septum thickness ≥20 mm; and "2" for both LVOT gradient ≥30 mm Hg and baseline septum thickness ≥20 mm. The C statistic of the ASA-SCARRE risk score was 0.684 (SE 0.030). In conclusion, the ASA-SCARRE risk score may be a useful and easily available clinical tool to predict risk of sudden cardiac arrest events after ASA in patients with hypertrophic obstructive cardiomyopathy.

Published
2022
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5. Outcomes of Patients With Hypertrophic Obstructive Cardiomyopathy and Pacemaker Implanted After Alcohol Septal Ablation

Author

Josef Veselka, Max Liebregts, Robert Cooper, Lothar Faber, Jaroslav Januska, Maksim Kashtanov, Klara Hulikova Tesarkova, Peter Riis Hansen, Hubert Seggewiss, Eugene Shloydo, Kirill Popov, Eva Hansvenclova, Jiri Bonaventura, Jurriën ten Berg, Rodney Hilton Stables, and Eva Polakova

Subjects
Ablation Techniques, Pacemaker, Artificial, Treatment Outcome, Ethanol, Heart Septum, Humans, Cardiomyopathy, Hypertrophic, Atrioventricular Block, Cardiology and Cardiovascular Medicine, Retrospective Studies
Abstract

Atrioventricular block is a frequent major complication after alcohol septal ablation (ASA).The aim of this study was to evaluate the outcomes of patients with implanted permanent pacemaker (PPM) related to a high-grade atrioventricular block after ASA for hypertrophic obstructive cardiomyopathy.We used a multinational registry (the Euro-ASA registry) to evaluate the outcome of patients with PPM after ASA.A total of 1,814 patients were enrolled and followed up for 5.0 ± 4.3 years (median = 4.0 years). A total of 170 (9.4%) patients underwent PPM implantation during the first 30 days after ASA. Using propensity score matching, 139 pairs (n = 278) constituted the matched PPM and non-PPM groups. Between the matched groups, there were no long-term differences in New York Heart Association functional class (1.5 ± 0.7 vs 1.5 ± 0.9, P = 0.99) and survival (log-rank P = 0.47). Patients in the matched PPM group had lower long-term left ventricular (LV) outflow gradient (12 ± 12 mm Hg vs 17 ± 19 mm Hg, P 0.01), more pronounced LV outflow gradient decrease (81% ± 17% vs 72% ± 35%, P 0.01), and lower LV ejection fraction (64% ± 8% vs 66% ± 8%, P = 0.02) and were less likely to undergo reintervention (re-ASA or myectomy) (log-rank P = 0.02).Patients with hypertrophic obstructive cardiomyopathy treated with ASA have a 9% probability of PPM implantation within 30 days after ASA. In long-term follow-up, patients with PPM had similar long-term survival and New York Heart Association functional class but lower LV outflow gradient, a more pronounced LV outflow gradient decrease, a lower LV ejection fraction, and a lower likelihood of reintervention compared with patients without PPM.

Published
2022
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10. Prediction of Sudden Cardiac Arrest After Alcohol Septal Ablation for Hypertrophic Obstructive Cardiomyopathy: ASA-SCARRE Risk Score

Author

Veselka, Josef, primary, Liebregts, Max, additional, Cooper, Robert, additional, Faber, Lothar, additional, Januska, Jaroslav, additional, Kashtanov, Maksim, additional, Tesarkova, Klara Hulikova, additional, Hansen, Peter Riis, additional, Seggewiss, Hubert, additional, Shloydo, Eugene, additional, Popov, Kirill, additional, Hansvenclova, Eva, additional, Polakova, Eva, additional, ten Berg, Jurriën, additional, Stables, Rodney Hilton, additional, Jarkovsky, Jiri, additional, and Bonaventura, Jiri, additional

Published
2022
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12. Immunophenotyping in pemphigus reveals a TH17/TFH17 cell–dominated immune response promoting desmoglein1/3-specific autoantibody production

Author

Alexandra Polakova, Christoph Hudemann, Farzan Solimani, Jakob Matthes, Kamran Ghoreschi, Iris Schäfer, Rüdiger Eming, Michael Hertl, Robert Pollmann, Christian Möbs, Julia Holstein, Dario Didona, Martin Dugas, Tobias Tekath, Carolin Baum, Nicolas Casadei, Wolfgang Pfützner, Katharina Meier, and Amir S. Yazdi

Subjects
0301 basic medicine, integumentary system, medicine.medical_treatment, Immunology, Pemphigus vulgaris, Autoantibody, Biology, medicine.disease, Desmoglein, 03 medical and health sciences, Pemphigus, 030104 developmental biology, 0302 clinical medicine, Immune system, Cytokine, Immunophenotyping, medicine, Immunology and Allergy, Pemphigus foliaceus, 030215 immunology
Abstract

Background TH2 cells were thought to be a pivotal factor for initiation of the autoimmune blistering disease pemphigus. However, the role of other T-cell subsets in pemphigus pathogenesis remained unclear. Objective We aimed to characterize the exact phenotype of T cells responsible for the development of pemphigus. Methods Whole transcriptome shotgun sequencing was performed to determine differential gene expression in pemphigus lesions and skin of healthy individuals. The cutaneous cytokine signature was further evaluated by real-time quantitative PCR. In peripheral blood, the distribution of TH cell and folliclular helper (TFH) cell subsets was analyzed by flow cytometry. Finally, the capacity of TH and TFH cell subsets to induce desmoglein (Dsg)-specific autoantibodies by memory B cells was evaluated in coculture experiments. Results Transcriptome analysis of skin samples identified an IL-17A–dominated immune signature in patients with pemphigus, and Kyoto Encyclopedia of Genes and Genomes pathway analysis confirmed the dominance of the IL-17A signaling pathway. Increased expression of IL17A and associated cytokines was also detected by real-time quantitative PCR comparing lesional with perilesional or healthy skin. Interestingly, utilization of flow cytometry showed that patients with active pemphigus had elevated levels of circulating IL-17+, TH17, TFH17, and TFH17.1 cells. Notably, levels of TH17 and TFH17 cells correlated with levels of Dsg-specific CD19+CD27+ memory B cells, and patients with acute pemphigus showed higher levels of Dsg3-autoreactive TFH17 cells. Coculture experiments revealed TFH17 cells as primarily responsible for inducing Dsg-specific autoantibody production by B cells. Conclusion Our findings show that TFH17 cells are critically involved in the pathogenesis of pemphigus and offer novel targets for therapeutic intervention.

Published
2021
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13. CML-184 A Novel Droplet Digital PCR Strategy for Rapid and Sensitive Detection of BCR::ABL1 Kinase Domain Mutations Conferring Resistance to Second-Generation Tyrosine Kinase Inhibitors

Author

de Santis, Sara, primary, Monaldi, Cecilia, additional, Martelli, Margherita, additional, Mancini, Manuela, additional, Bruno, Samantha, additional, Castagnetti, Fausto, additional, Gugliotta, Gabriele, additional, Polakova, Katerina Machova, additional, Ernst, Thomas, additional, Maar, Dianna, additional, Corner, Adam, additional, Cavo, Michele, additional, and Soverini, Simona, additional

Published
2022
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14. Poster: CML-184 A Novel Droplet Digital PCR Strategy for Rapid and Sensitive Detection of BCR::ABL1 Kinase Domain Mutations Conferring Resistance to Second-Generation Tyrosine Kinase Inhibitors

Author

de Santis, Sara, primary, Monaldi, Cecilia, additional, Martelli, Margherita, additional, Mancini, Manuela, additional, Bruno, Samantha, additional, Castagnetti, Fausto, additional, Gugliotta, Gabriele, additional, Polakova, Katerina Machova, additional, Ernst, Thomas, additional, Maar, Dianna, additional, Corner, Adam, additional, Cavo, Michele, additional, and Soverini, Simona, additional

Published
2022
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15. Outcomes of Patients With Hypertrophic Obstructive Cardiomyopathy and Pacemaker Implanted After Alcohol Septal Ablation

Author

Veselka, Josef, primary, Liebregts, Max, additional, Cooper, Robert, additional, Faber, Lothar, additional, Januska, Jaroslav, additional, Kashtanov, Maksim, additional, Tesarkova, Klara Hulikova, additional, Hansen, Peter Riis, additional, Seggewiss, Hubert, additional, Shloydo, Eugene, additional, Popov, Kirill, additional, Hansvenclova, Eva, additional, Bonaventura, Jiri, additional, Berg, Jurriën ten, additional, Stables, Rodney Hilton, additional, and Polakova, Eva, additional

Published
2022
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17. Effectiveness of alcohol septal ablation for hypertrophic obstructive cardiomyopathy in patients with late gadolinium enhancement on cardiac magnetic resonance

Author

Jiří Bonaventura, Natália Marková, Basak Kara, Theodor Adla, Josef Veselka, Max Liebregts, Eva Polakova, and Jurriën M. ten Berg

Subjects
medicine.medical_specialty, Alcohol septal ablation, Contrast Media, Ventricular outflow tract obstruction, Gadolinium, 030204 cardiovascular system & hematology, Obstructive cardiomyopathy, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, Late gadolinium enhancement, cardiovascular diseases, 030212 general & internal medicine, Interventricular septum, Retrospective Studies, business.industry, Hypertrophic cardiomyopathy, Retrospective cohort study, Cardiomyopathy, Hypertrophic, medicine.disease, Magnetic Resonance Imaging, medicine.anatomical_structure, embryonic structures, Cardiology, medicine.symptom, Cardiology and Cardiovascular Medicine, business, Cardiac magnetic resonance
Abstract

Background According to European guidelines, alcohol septal ablation (ASA) for hypertrophic obstructive cardiomyopathy (HOCM) may be less effective in patients with extensive septal scarring on cardiac magnetic resonance (CMR). This study aimed to analyze the impact of late gadolinium enhancement (LGE) on CMR on the effectiveness of ASA. Method We conducted an observational retrospective study involving adult patients with symptomatic drug-refractory HOCM who underwent CMR before ASA at two European centres from May 2010 through June 2019. Patients were compared in binary format based on LGE presence. Moreover, a subanalysis focused on patients with septal fibrosis was performed. The effectiveness of ASA was evaluated by echocardiographic, ECG and clinical findings. Results Of the 113 study patients, 54 (48%) had LGE on CMR. The LGE quantification performed in 29 patients revealed septal fibrosis in 17. The mean follow-up was 4.4 ± 2.6 years. Baseline parameters were similar between groups except for basal septal thickness that was greater in LGE+ group (21.1 ± 3.9 mm for LGE+ vs. 19.2 ± 3.2 mm for LGE-: p = .005). ASA improved symptoms in all groups and reduced left ventricular outflow tract obstruction (LVOTO) (delta gradient reduction: LGE+: 62 ± 37.3%; septal LGE+: 75.6 ± 20.8%; LGE-: 72.5 ± 21.0%). However, 13% of the LGE+ and 2% of the LGE- group had residual LVOTO above 30 mmHg (p = .027). Conclusion ASA was effective in all patients with HOCM, whether they had LGE on CMR or not and whether they had septal fibrosis or not.

Published
2020
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19. CML-184 A Novel Droplet Digital PCR Strategy for Rapid and Sensitive Detection of BCR::ABL1 Kinase Domain Mutations Conferring Resistance to Second-Generation Tyrosine Kinase Inhibitors

Author

Sara de Santis, Cecilia Monaldi, Margherita Martelli, Manuela Mancini, Samantha Bruno, Fausto Castagnetti, Gabriele Gugliotta, Katerina Machova Polakova, Thomas Ernst, Dianna Maar, Adam Corner, Michele Cavo, and Simona Soverini

Subjects
Cancer Research, Oncology, Hematology
Published
2022
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20. Worldwide Disparities in Recovery of Cardiac Testing 1 Year Into COVID-19

Author

Einstein, Andrew J., primary, Hirschfeld, Cole, additional, Williams, Michelle C., additional, Vitola, Joao V., additional, Better, Nathan, additional, Villines, Todd C., additional, Cerci, Rodrigo, additional, Shaw, Leslee J., additional, Choi, Andrew D., additional, Dorbala, Sharmila, additional, Karthikeyan, Ganesan, additional, Lu, Bin, additional, Sinitsyn, Valentin, additional, Ansheles, Alexey A., additional, Kudo, Takashi, additional, Bucciarelli-Ducci, Chiara, additional, Nørgaard, Bjarne Linde, additional, Maurovich-Horvat, Pál, additional, Campisi, Roxana, additional, Milan, Elisa, additional, Louw, Lizette, additional, Allam, Adel H., additional, Bhatia, Mona, additional, Sewanan, Lorenzo, additional, Malkovskiy, Eli, additional, Cohen, Yosef, additional, Randazzo, Michael, additional, Narula, Jagat, additional, Morozova, Olga, additional, Pascual, Thomas N.B., additional, Pynda, Yaroslav, additional, Dondi, Maurizio, additional, Paez, Diana, additional, Einstein, Andrew J., additional, Hinterleitner, Gerd, additional, Lu, Yao, additional, Xu, Zhuoran, additional, Hirschfeld, Cole B., additional, Erinne, Ikenna, additional, Shetty, Mrinali, additional, Choi, Andrew, additional, Lopez-Mattei, Juan, additional, Parwani, Purvi, additional, Goda, Artan, additional, Shirka, Ervina, additional, Bouyoucef, Salah, additional, Chelghoum, Lydia, additional, Mansouri, Farouk, additional, Medjahedi, Abdelkader, additional, Naili, Qais, additional, Ridouh, Mokhtar, additional, Alasia, Diego, additional, Alberghina, Lucia, additional, Aramayo, Natalia, additional, Buchara, Diego, additional, Busso, Franco Gabriel, additional, Bustos Rivadero, Jose Javier, additional, Camilletti, Jorge, additional, Campanelli, Hugo, additional, Castro, Ricardo Belisario, additional, Daicz, Mariana, additional, del Riego, Horacio, additional, Dragonetti, Laura, additional, Echazarreta, Diego, additional, Erriest, Juan, additional, Faccio, Fernando, additional, Facello, Adolfo, additional, Gallegos, Hugo, additional, Geronazzo, Ricardo, additional, Glait, Horacio, additional, Hasbani, Victor, additional, Jäger, Victor, additional, Lewkowicz, Julio Manuel, additional, Lotti, Jose, additional, Maciel, Neiva, additional, Masoli, Osvaldo, additional, Mastrovito, Edgardo, additional, Medus, Maria, additional, Merani, Maria Fernanda, additional, Molteni, Susana, additional, Montecinos, Marcos, additional, Parisi, Gustavo, additional, Sueldo, Claudio Pereyra, additional, Perez de Arenaza, Diego, additional, Quintana, Luis, additional, Radzinschi, Alejandro, additional, Redruello, Marcela, additional, Rodríguez, Marina, additional, Rojas, Horacio, additional, Acuña, Arturo Romero, additional, Schere, Daniel, additional, Traverso, Sonia, additional, Vazquez, Gustavo, additional, Zeffiro, Susana, additional, Sakanyan, Mari, additional, Beuzeville, Scott, additional, Boktor, Raef, additional, Crowley, Michael, additional, Downie, D'Arne, additional, Dwivedi, Girish, additional, Elison, Barry, additional, Farouque, Omar, additional, Jasper, Kim, additional, Joshi, Subodh, additional, Lee, Joseph, additional, Lee, Kenneth, additional, Lui, Elaine, additional, Mcconachie, Peter, additional, Meaker, Joanne, additional, Nandurkar, Dee, additional, Neill, Johanne, additional, O'Rourke, Edward, additional, O'Sullivan, Patricia, additional, Pandos, George, additional, Premaratne, Manuja, additional, Prior, David, additional, Rutherford, Natalie, additional, Saunders, Connor, additional, Taubman, Kim, additional, Tauro, Andrew, additional, Taylor, Andrew, additional, Theuerle, James, additional, Thomas, Paul, additional, Tow, Jonathan, additional, Upton, Anthony, additional, Vamadevan, Shankar, additional, Wayne, Victor, additional, Wegner, Eva Alina, additional, Wong, David, additional, Younger, John, additional, Beitzke, Dietrich, additional, Feuchtner, Gudrun, additional, Sommer, Oliver, additional, Weiss, Konrad, additional, Maroz-Vadalazhskaya, Natallia, additional, Tserakhau, Uladzimir, additional, Homans, Filip, additional, Van De Heyning, Caroline M., additional, Araujo, Raúl, additional, Soldat-Stankovic, Valentina, additional, Stankovic, Sinisa, additional, Almeida, Augusto, additional, Anselmi, Carlos, additional, Azevedo, Guilherme S.A., additional, Bittencourt, Marcio Sommer, additional, Pianta, Diego Bromfman, additional, Cabeda, Estevan, additional, Carreira, Lara, additional, Coelho, Igor, additional, de Amorim Fernandes, Fernando, additional, de Lorenzo, Andrea, additional, Delgado, Roberta, additional, Erthal, Fernanda, additional, Fernandes, Fabio, additional, Fernandes, Juliano, additional, Ferreira de Souza, Thiago, additional, Foppa, Murilo, additional, Matos Alves, Wilson Furlan, additional, Gontijo, Cibele, additional, Gottlieb, Ilan, additional, Grossman, Gabriel, additional, Albernaz Siqueira, Maria Helena, additional, Nomura, Cesar Higa, additional, Koga, Katia Hiromoto, additional, Lima, Ronaldo, additional, Lopes, Rafael, additional, Marçal Filho, Hugo Humberto, additional, Masiero, Paulo, additional, Mastrocola, Luiz, additional, Menezes de Siqueira, Maria Eduarda, additional, Mesquita, Claudio, additional, Naves, Danilo, additional, Penna, Filipe, additional, Pinto, Ibraim, additional, Rocha, Thércio, additional, Rocha, Juliana Leal, additional, Rodrigues, Alfredo, additional, Salioni, Leila, additional, Sanches, Adelina, additional, Santos, Marcelo, additional, Da Silva, Leonardo Sara, additional, Schvartzman, Paulo, additional, Matushita, Cristina Sebastião, additional, Senra, Tiago, additional, Silva, Marcelo, additional, Soares, Carlos Eduardo, additional, Spiro, Bernardo, additional, Suaide Silva, Carlos Eduardo, additional, Torres, Rafael, additional, Monte, Guilherme Urpia, additional, Vilela, Andrea, additional, Villa, Alexandre Volney, additional, Vitola, Joao, additional, Voss, Themissa, additional, Waltrick, Roberto, additional, Zapparoli, Marcello, additional, Naseer, Hamid, additional, Garcheva-Tsacheva, Marina, additional, Ouattara, Tiémégna Florence, additional, Thou, Sarameth, additional, Varoeun, Soley, additional, Abikhzer, Gad, additional, Beanlands, Rob, additional, Chetrit, Michael, additional, Dabreo, Dominique, additional, Dennie, Carole, additional, Friedrich, Matthias, additional, Hafez, Mohmmed Nassoh, additional, Hanneman, Kate, additional, Miller, Robert, additional, Oikonomou, Anastasia, additional, Roifman, Idan, additional, Small, Gary, additional, Tandon, Vikas, additional, Trivedi, Adwait, additional, White, James, additional, Zukotynski, Katherine, additional, Alay, Rita, additional, Concha, Carmen, additional, Massardo, Teresa, additional, Abad, Pedro, additional, Anzola, Kelly, additional, Arturo, Harold, additional, Benitez, Luis, additional, Cadena, Alberto, additional, Zamudio, Carlos Caicedo, additional, Calderón, Antonio, additional, Gutierrez Villamil, Claudia T., additional, Jaimes, Claudia, additional, Londono, Juan L., additional, Lopez, Nelson, additional, Merlano-Gaitan, Sonia, additional, Murgieitio-Cabrera, Ramon, additional, Valencia, Manuel, additional, Vergel, Damiana, additional, Santamaria, Alejandro Zuluaga, additional, Solis, Felix, additional, Batinic, Tonci, additional, Franceschi, Maja, additional, Paar, Maja Hrabak, additional, Prpic, Marina, additional, Felipe Batista, Cuba: Juan, additional, Cabrera, Lazaro Omar, additional, Peix, Amalia, additional, Peña, Yamilé, additional, Rochela Vázquez, Luis Manuel, additional, Ntalas, Ioannis, additional, Kaminek, Milan, additional, Kincl, Vladimir, additional, Lang, Otto, additional, Abdulla, Jawdat, additional, Bøttcher, Morten, additional, Busk, Martin, additional, Geisler, Uka, additional, Gormsen, Lars C., additional, Hansson, Nicolaj, additional, Hess, Søren, additional, Hove, Jens, additional, Jensen, Lars Thorbjoern, additional, Jensen, Magnus T., additional, Kragholm, Kristian Hay, additional, Nørgaard, Bjarne L., additional, Øvrehus, Kristian, additional, Rasmussen, Jan, additional, Rønnow Sand, Niels Peter, additional, Sondergaard, Hanne, additional, Zaremba, Tomas, additional, Speckter, Herwin, additional, Amores, Nelson, additional, Velez, Mayra Sanchez, additional, Alrahman, Taghreed Abd, additional, Elsamad, Sherif Abd, additional, Abdelfattah, Alia, additional, Allam, Adel, additional, Elkaffas, Sameh, additional, Hassan, Mona, additional, Hussein, Elshaymaa, additional, Ibrahim, Ahmed, additional, Kandeel, Ahmed, additional, Ali, Mohamed Mandour, additional, Shaaban, Mahmoud, additional, Flores, Camila, additional, Gómez Leiva, Verónica Vanesa, additional, Liiver, Anita, additional, Larikka, Martti, additional, Uusitalo, Valtteri, additional, Agostini, Denis, additional, Berger, Clothilde, additional, Dietz, Matthieu, additional, Hyafil, Fabien, additional, Ohana, Mickaël, additional, Prigent, Kevin, additional, Regaieg, Hamza, additional, Sarda-Mantel, Laure, additional, H-Ici, Darach O., additional, Ayetey, Harold, additional, Angelidis, George, additional, Fragkaki, Christina, additional, Fragkiadaki, Chrysoula, additional, Georgoulias, Panagiotis, additional, Koutelou, Maria, additional, Kyrozi, Elena, additional, Lama, Niki, additional, Prassopoulos, Vassilis, additional, Spartalis, Michael, additional, Zaglavara, Theodora, additional, Gonzalez, Carla, additional, Gutierrez, Goleat, additional, Maldonado, Alejandro, additional, Martinez, Yassine, additional, Kovács, Attila, additional, Szilveszter, Bálint, additional, Banthia, Nilesh, additional, Bhat, Vivek, additional, Choudhury, Partha, additional, Chowdekar, Vijay Sai, additional, Christopher, Johann, additional, Garg, Tushar, additional, Goyal, Naresh Kumar, additional, Gupta, Ripen Kumar, additional, Gupta, Abhishek, additional, Hephzibah, Julie, additional, Jain, Shashank, additional, Krupa, Jesu, additional, Kumar, Parveen, additional, Kumar, Sukriti, additional, Lalchandani, Arati, additional, Mishra, Animesh, additional, Mishra, Vivaswan Dutt, additional, Mohan, Parul, additional, Ozair, Ahmad, additional, Pandey, Shivani, additional, Parameswaran, Ramanathapuram, additional, Patel, Chetan, additional, Patel, Tapan, additional, Patel, Shivani, additional, Vimala, Leena Robinson, additional, Kumar Sarangi, Dr Pradosh, additional, Sengupta, Shantanu, additional, Sethi, Arvind, additional, Sharma, Amit, additional, Sharma, Awadhesh Kumar, additional, Sharma, Punit, additional, Shrigiriwar, Apurva, additional, Singh, Santosh, additional, Singh, Harpreet, additional, Sood, Ashwani, additional, Verma, Atul, additional, Vyas, Ajay, additional, Soeriadi, Erwin Affandi, additional, Bun, Edison, additional, Hutomo, Febby, additional, Syawaluddin, Hilman, additional, Yudistiro, Ryan, additional, Albadr, Amjed, additional, Assadi, Majid, additional, Emami, Farshad, additional, Emami-Ardekani, Alireza, additional, Farzanehfar, Saeed, additional, Jafari, Ramezan, additional, Manafi-Farid, Reyhaneh, additional, Tajik, Maryam, additional, Arnson, Yoav, additional, Fuchs, Shmuel, additional, Goldkorn, Ronen, additional, Kennedy, John, additional, Leitman, Marina, additional, Shalev, Aryeh, additional, Acampa, Wanda, additional, Albano, Domenico, additional, Alongi, Pierpaolo, additional, Arnone, Gaspare, additional, Assante, Roberta, additional, Baritussio, Anna, additional, Bauckneht, Matteo, additional, Bianco, Francesco, additional, Bonfiglioli, Rachele, additional, Bovenzi, Francesco, additional, Bruno, Isabella, additional, Bruno, Andrea, additional, Busnardo, Elena, additional, Califaretti, Elena, additional, Casoni, Roberta, additional, Censullo, Vittorio, additional, Chierichetti, Franca, additional, Chiocchi, Marcello, additional, Cittanti, Corrado, additional, Clemente, Alberto, additional, Cuocolo, Alberto, additional, De Rimini, Maria Luisa, additional, De Vincentis, Giuseppe, additional, Della Tommasina, Veronica, additional, Dellegrottaglie, Santo, additional, Erba, Paola Anna, additional, Evangelista, Laura, additional, Faggi, Lara, additional, Faragasso, Evelina, additional, Florimonte, Luigia, additional, Frantellizzi, Viviana, additional, Gatti, Marco, additional, Gaudiano, Angela, additional, Gelardi, Fabrizia, additional, Gerali, Alberto, additional, Gimelli, Alessia, additional, Guglielmo, Marco, additional, Leccisotti, Lucia, additional, Liga, Riccardo, additional, Liguori, Carlo, additional, Longo, Giampiero, additional, Maffione, Margherita, additional, Marcassa, Claudio, additional, Matassa, Giovanni, additional, Mele, Donato, additional, Mircoli, Luca, additional, Paccagnella, Andrea, additional, Pacella, Sara, additional, Padovano, Federica, additional, Pellegrini, Dario, additional, Pergola, Valeria, additional, Pugliese, Luca, additional, Quartuccio, Natale, additional, Rampin, Lucia, additional, Ricci, Fabrizio, additional, Rubini, Giuseppe, additional, Russo, Vincenzo, additional, Sambuceti, Gianmario, additional, Scatteia, Alessandra, additional, Sciagrà, Roberto, additional, Spidalieri, Gianluca, additional, Stefanelli, Antonella, additional, Tedeschi, Carlo, additional, Ventroni, Guido, additional, Baugh, Dainia, additional, Madu, Ernest, additional, Aikawa, Tadao, additional, Asano, Hiroshi, additional, Fujimoto, Shinichiro, additional, Fujise, Koichiro, additional, Fukushima, Yoshimitsu, additional, Fukuyama, Kae, additional, Ichikawa, Yasutaka, additional, Ideguchi, Reiko, additional, Iguchi, Nobuo, additional, Imai, Masamichi, additional, Ishimura, Hayato, additional, Isobe, Satoshi, additional, Ito, Kimiteru, additional, Izawa, Yu, additional, Kadokami, Toshiaki, additional, Kasai, Tokuo, additional, Kato, Takao, additional, Kawamoto, Takashi, additional, Kiryu, Shigeru, additional, Kumita, Shinichiro, additional, Manabe, Osamu, additional, Maruno, Hirotaka, additional, Matsumoto, Naoya, additional, Miyagawa, Masao, additional, Moroi, Masao, additional, Nagamachi, Shigeki, additional, Nakajima, Kenichi, additional, Nakazato, Ryo, additional, Nanasato, Mamoru, additional, Naya, Masanao, additional, Norikane, Takashi, additional, Ohta, Yasutoshi, additional, Otomi, Yoichi, additional, Otsuka, Hideki, additional, Oyama-Manabe, Noriko, additional, Saito, Masaki, additional, Sarai, Masayoshi, additional, Sato, Junichi, additional, Sato, Daisuke, additional, Shiraishi, Shinya, additional, Takanami, Kentaro, additional, Takehana, Kazuya, additional, Taniguchi, Yasuyo, additional, Teragawa, Hiroki, additional, Tomizawa, Nobuo, additional, Umeji, Kyoko, additional, Wakabayashi, Yasushi, additional, Yamada, Shinichiro, additional, Yamazaki, Shinya, additional, Yoneyama, Tatsuya, additional, Rawashdeh, Mohammad, additional, Dautov, Tairkhan, additional, Makhdomi, Khalid, additional, Abass, Mostafa, additional, Garashi, Masoud, additional, Siraj, Qaisar, additional, Kalnina, Marika, additional, Haidar, Mohamad, additional, Komiagiene, Renata, additional, Kviecinskiene, Giedre, additional, Vajauskas, Donatas, additional, Karim, Noor Khairiah A., additional, Doucoure, Mady, additional, Reichmuth, Luise, additional, Samuel, Anthony, additional, Dieng, Mohamed Lemine, additional, Naojee, Ambedhkar Shantaram, additional, Hernandez, Estrella Aguilera, additional, Alducin Tellez, Cesar Rene, additional, Alexánderson-Rosas, Erick, additional, Barragan, Erika, additional, Cabada, Manuel, additional, Calderón, Daniel, additional, Carvajal-Juarez, Isabel, additional, Esparza, José, additional, Gama-Moreno, Manlio Gerardo, additional, Quinto, Virginia Garcia, additional, Gonzalez, Nelsy Coromoto, additional, Herrera-Zarza, Mary Carmen, additional, Meave, Aloha, additional, Medina Verdugo, Jesus Gregorio, additional, Melendez, Gabriela, additional, Morales Murguia, Rafael Humberto, additional, Navarro Quiroz, Carlos Salvador, additional, Ornelas, Mario, additional, Preciado-Anaya, Andres, additional, Preciado-Gutiérrez, Oscar Ulises, additional, Puente, Adriana, additional, Salazar, Aristóteles Ramírez, additional, Rosales Uvera, Sandra Graciela, additional, Rosales-Uvera, Sandra, additional, Serna Macias, Jose Antonio, additional, Sierra-Galan, Lilia, additional, Sierra-Galan, Lilia M., additional, Tirado Alderete, Juan Carlos, additional, Vallejo, Enrique, additional, Faraggi, Marc, additional, Sereegotov, Erdenechimeg, additional, Ben Rais, Nouzha, additional, Alaoui, Nadia Ismaili, additional, Kyiphyu, Thiri, additional, Oo, Su Thet, additional, Win, Soe Myat, additional, Zar, Htin, additional, Ghimire, Ram, additional, Neupane, Madhu, additional, Glaudemans, Andor, additional, Slart, Riemer, additional, Verschure, Derk, additional, Allen, Berry, additional, Edmond, John, additional, Mckenzie, Clare, additional, Tie, Stuart, additional, Van Pelt, Niels, additional, Worthington, Kirsten, additional, Young, Calum, additional, Soli, Idrissa Adamou, additional, Kana, Shehu, additional, Onubogu, Uchenna, additional, Sani, Mahmoud, additional, Bråten, Anders Tjellaug, additional, Jørgensen, Arve, additional, Vassbotn, Hanne-Elin, additional, Al Dhuhli, Humoud, additional, Jawa, Zabah, additional, Tag, Naima, additional, Fatima, Shazia, additional, Imran, Muhammad Babar, additional, Younis, Muhammad Numair, additional, Saadullah, Mohammad, additional, Malo, Yariela Herrera, additional, Lenturut-Katal, Dora, additional, Castillo, Manuel, additional, Ortellado, José, additional, Akhter, Afroza, additional, Cader, F. Aaysha, additional, Hussain, Raihan, additional, Khan, Saidur Rahman, additional, Mandal, Tapati, additional, Nasreen, Faria, additional, An, Yunqiang, additional, Cao, Dianbo, additional, Gong, Lianggeng, additional, Hou, Yang, additional, Jia, Chongfu, additional, Li, Tao, additional, Li, Caiying, additional, Liu, Hui, additional, Liu, Wenya, additional, Liu, Jinkang, additional, Ng, Ming-Yen, additional, Shi, Heshui, additional, Tang, Chunxiang, additional, Wang, Ximing, additional, Wang, Zhaoqian, additional, Wang, Yining, additional, Wu, Jiang, additional, Yi, Yan, additional, Yuan, Li, additional, Zhang, Tong, additional, Zhang, Longjiang, additional, Chavez, Edith, additional, Cruz, Carlos, additional, Llontop, Christian, additional, Morales, Rosanna, additional, Abrihan, Paz, additional, Bustos-Barroso, Asela, additional, Duldulao-Ogbac, Michele, additional, Eduarte, Christopher, additional, Obaldo, Jerry, additional, Quinon, Alvin, additional, San Juan, Belinda, additional, San Juan, Carlo Joe, additional, Sauler-Gomez, Marie Rhiamar, additional, Uy, Mila, additional, Kostkiewicz, Magdalena, additional, Kunikowska, Jolanta, additional, Teresinska, Anna, additional, Urbanik, Tomasz, additional, Bettencourt, Nuno, additional, Fontes-Carvalho, Ricardo, additional, Gavina, Cristina, additional, Gonçalves, Lino, additional, Macedo, Filipe, additional, Moreno, Nuno, additional, Sousa, Carla, additional, Timoteo, Ana Teresa, additional, Vidigal, Maria João, additional, Al Heidous, Mahmoud, additional, Ramanathan, Subramaniyan, additional, Arnous, Samer, additional, Aytani, Said, additional, Byrne, Angela, additional, Gleeson, Tadhg, additional, Kerins, David, additional, O'Brien, Julie, additional, Bang, Ji-In, additional, Bom, Henry, additional, Cheon, Miju, additional, Cheon, Gi Jeong, additional, Cho, Sang-Geon, additional, Hong, Chae Moon, additional, Jeong, Yong Hyu, additional, Kang, Won Jun, additional, Kang, Yeon-Koo, additional, Kim, Ji-Young, additional, Oh, So Won, additional, So, Young, additional, Song, Ho-Chun, additional, Won, Kyoung Sook, additional, Yoo, Soo Woong, additional, Mitevska, Irena, additional, Vavlukis, Marija, additional, Salobir, Barbara Gužic, additional, Štalc, Monika, additional, Benedek, Theodora, additional, Pop, Marian, additional, Stan, Claudiu, additional, Ansheles, Alexey, additional, Dariy, Olga, additional, Gagarina, Nina, additional, Itskovich, Irina, additional, Karalkin, Anatoliy, additional, Kokov, Alexander, additional, Marina, Gulya, additional, Migunova, Ekaterina, additional, Pospelov, Viktor, additional, Ryzhkova, Daria, additional, Sayfullina, Guzaliya, additional, Sergienko, Vladimir, additional, Shurupova, Irina, additional, Vakhromeeva, Margarita, additional, Valiullina, Nailia, additional, Zavadovsky, Konstantin, additional, Zhuravlev, Kirill, additional, Abazid, Rami, additional, Al Garni, Turki, additional, Alasnag, Mirvat, additional, Aljizeeri, Ahmed, additional, Amer, Hamid, additional, Amro, Ahmad, additional, Hamdy, Hesham, additional, Smettei, Osama, additional, Saranovic, Dragana Sobic, additional, Vlajkovic, Marina, additional, Keng, Felix, additional, See, Jason, additional, Berecova, Zuzana, additional, Mistinova, Jana Polakova, additional, Evbuomwan, Osayande, additional, Govender, Nerisha, additional, Hack, Jonathan, additional, Hadebe, Bawinile, additional, Hlongwa, Khanyisile, additional, Kaplan, Mitchell, additional, Lakhi, Hoosen, additional, Milos, Katarina, additional, Modiselle, Moshe, additional, More, Stuart, additional, Muambadzi, Ntanganedzeni, additional, Scholtz, Leonie, additional, Barreiro-Perez, Manuel, additional, Blanco, Isabel, additional, Broncano, Jordi, additional, Camarero, Alicia, additional, Casáns-Tormo, Irene, additional, De Haro, Javier, additional, Flotats, Albert, additional, García, Elia, additional, Mendiguchia, Ceferino Gutierrez, additional, Jimenez-Heffernan, Amelia, additional, Leta, Ruben, additional, Diaz, Javier Lopez, additional, Vega, Luis Lumbreras, additional, Manovel-Sánchez, Ana, additional, Monzonis, Amparo Martinez, additional, Patrut, Bianca, additional, Pubul, Virginia, additional, Perez, Ricardo Ruano, additional, Zeidan, Nahla, additional, Nanayakkara, Damayanthi, additional, Suliman, Ahmed, additional, Engblom, Henrik, additional, Murtadha, Mustafa, additional, Ostenfeld, Ellen, additional, Simonsson, Magnus, additional, Alkadhi, Hatem, additional, Buechel, Ronny Ralf, additional, Burger, Peter, additional, Gräni, Christoph, additional, Kamani, Christel, additional, Kawel-Böhm, Nadine, additional, Klaeser, Bernd, additional, Manka, Robert, additional, Prior, John, additional, Kaewchur, Tawika, additional, Khiewvan, Benjapa, additional, Kositwattanarerk, Arpakorn, additional, Namwongprom, Sirianong, additional, Thientunyakit, Tanyaluck, additional, Sayman, Haluk Burcak, additional, Yüksel, Mahmut, additional, Sebikali, Mugisha Julius, additional, Okello, Emmy, additional, Korol, Pavlo, additional, Noverko, Iryna, additional, Satyr, Maryna, additional, Ahmad, Tahir, additional, Alfakih, Khaled, additional, Andrade, Ivo, additional, Buckingham, Susan, additional, Bularga, Anda, additional, Carpenter, John-Paul, additional, Cole, Graham, additional, Cusack, David, additional, David, Sarojini, additional, Davis, Patrick, additional, Fairbairn, Timothy, additional, Ghosh, Arjun, additional, Ramkumar, Prasad Guntur, additional, Hamilton, Mark, additional, Haque, Faisal, additional, Hudson, Benjamin, additional, Johnstone, Annette, additional, Karthikeyan, V.J., additional, Kay, Mike, additional, Khan, Mohammad Ali, additional, Kitt, Jamie, additional, Low, Chen Sheng, additional, Mcalindon, Elisa, additional, Mccreavy, David, additional, Morrissey, Brian, additional, Motwani, Manish, additional, Na, Dilip, additional, Nicol, Edward, additional, Patel, Dilip, additional, Rodrigues, Jonathan, additional, Rofe, Chris, additional, Schofield, Rebecca, additional, Semple, Thomas, additional, Sheikh, Azeem, additional, Sinha, Apurva, additional, Subedi, Deepak, additional, Topping, William, additional, Tweed, Katherine, additional, Underwood, Stephen Richard, additional, Weir-Mccall, Jonathan, additional, Zuhairy, Hamed, additional, Abbasi, Taimur, additional, Abohashem, Shady, additional, Abramson, Sandra, additional, Al-Mallah, Mouaz, additional, Kumar, Mohan Ashok, additional, Balmer-Swain, Mallory, additional, Berman, Daniel, additional, Bernheim, Adam, additional, Bhatti, Sabha, additional, Biederman, Robert, additional, Bieging, Erik, additional, Bingham, Scott, additional, Bloom, Stephen, additional, Blue, Sean, additional, Borges, Andressa, additional, Branch, Kelley, additional, Bravo, Paco, additional, Buddhe, Sujatha, additional, Budoff, Matthew, additional, Bullock-Palmer, Renée, additional, Cahill, Michael, additional, Candela, Candace, additional, Cao, Jane, additional, Chatterjee, Saurav, additional, Chatzizisis, Yiannis, additional, Chaudhuri, Nita Ray, additional, Cheezum, Michael, additional, Chelliah, Anjali, additional, Chen, Tiffany, additional, Chen, Marcus, additional, Chen, Lu, additional, Chokshi, Aalap, additional, Chung, Jina, additional, Danciu, Sorin, additional, DeSisto, William, additional, Dilorenzo, Michael, additional, Doukky, Rami, additional, Duvall, William, additional, Ferencik, Maros, additional, Foster, Cameron, additional, Fuisz, Anthon, additional, Gannon, Michael, additional, German, David, additional, Gerson, Myron, additional, Geske, Jeffrey, additional, Hage, Fadi, additional, Haider, Agha, additional, Haider, Sofia, additional, Hamirani, Yasmin, additional, Hassen, Karen, additional, Hendel, Robert, additional, Henkel, Jacqueline, additional, Horgan, Stephen, additional, Hyun, Mark, additional, Janardhanan, Rajesh, additional, Jerome, Scott, additional, Kalra, Dinesh, additional, Kassop, David, additional, Kinkhabwala, Mona, additional, Kinzfogl, George, additional, Koch, Bernard, additional, Koweek, Lynne, additional, Krepp, Joseph, additional, Kwon, Younghoon, additional, Layer, Jay, additional, Lesser, John, additional, Leung, Steve, additional, Lisske, Bernadette, additional, Magurany, Kathleen, additional, Markowitz, Jeremy, additional, Mccullough, Brenda, additional, Moalemi, Azita, additional, Moffitt, Chanan, additional, Montanez, Juan, additional, Moore, Warren, additional, Morayati, Shamil, additional, Mossa-Basha, Mahmud, additional, Mrsic, Zorana, additional, Murthy, Venkatesh, additional, Nagpal, Prashant, additional, Nelson, Katarina, additional, Nijjar, Prabhjot, additional, O’Quinn, Rupal, additional, Passen, Edward, additional, Patel, Toral, additional, Patil, Pravin, additional, Pursnani, Amit, additional, Quachang, Nancy, additional, Rabbat, Mark, additional, Ranjan, Pragya, additional, Lozano, Patricia Rodriguez, additional, Schemmer, Mary, additional, Seifried, Rebecca, additional, Shah, Nishant, additional, Shah, Amee, additional, Shanbhag, Sujata, additional, Sharma, Gaurav, additional, Skotnicki, Robert, additional, Sobczak, Michael, additional, Soman, Prem, additional, Sorrell, Vincent, additional, Srichai, Monvadi, additional, Streeter, Jim, additional, Strickland, Leah, additional, Suliman, Suliman, additional, Tebyanian, Naghmeh, additional, Thomas, Dustin, additional, Thompson, Randall, additional, Uretsky, Seth, additional, Vallurupalli, Srikanth, additional, Vandyck-Acquah, Marian, additional, Verma, Vikas, additional, Villines, Todd, additional, Weinstein, Joseph, additional, Wolinsky, David, additional, Zareba, Karolina, additional, Zgaljardic, Michael, additional, Beretta, Mario, additional, Ferrando, Rodolfo, additional, Kapitan, Miguel, additional, Mut, Fernando, additional, Djuraev, Omoa, additional, Rozikhodjaeva, Gulnora, additional, Vera, Luisa, additional, Duc, Binh Duong, additional, Nguyen, Xuan Canh, additional, and Hiep Nguyen, Phuoc Minh, additional

Published
2022
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21. Isolation of Lymphocytes from Human Skin and Murine Tissues: A Rapid and Epitope-Preserving Approach

Author

Alexandra Polakova, Christoph Hudemann, Felix Wiemers, Arturas Kadys, Niklas Gremke, Manuel Lang, Lutz Zwiorek, Wolfgang Pfützner, Michael Hertl, Christian Möbs, Christine L. Zimmer, and Medizin

Subjects
Medizin, General Engineering, ddc:610, Medical sciences Medicine
Abstract

Tissue-resident immune cells have been shown to play an important role in skin health and disease. However, owing to limited access to human skin samples and time-consuming, technically demanding protocols, the characterization of tissue-derived cells remains challenging. For this reason, blood-derived leukocytes are frequently used as a surrogate specimen, although they do not necessarily reflect local immune responses in the skin. Therefore, we aimed to establish a rapid protocol to isolate a sufficient number of viable immune cells from 4-mm skin biopsies that can be directly used for a deeper characterization such as comprehensive phenotyping and functional studies of T cells. In this optimized protocol, only two enzymes, type IV collagenase and DNase I, were used to achieve both the highest possible cellular yield and marker preservation of leukocytes stained for multicolor flow cytometry. We further report that the optimized protocol may be used in the same manner for murine skin and mucosa. In summary, this study allows a rapid acquisition of lymphocytes from human or mouse skin suitable for comprehensive analysis of lymphocyte subpopulations, for disease surveillance, and for identification of potential therapeutic targets or other downstream applications.

Published
2023
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22. Poster: CML-184 A Novel Droplet Digital PCR Strategy for Rapid and Sensitive Detection of BCR::ABL1 Kinase Domain Mutations Conferring Resistance to Second-Generation Tyrosine Kinase Inhibitors

Author

Sara de Santis, Cecilia Monaldi, Margherita Martelli, Manuela Mancini, Samantha Bruno, Fausto Castagnetti, Gabriele Gugliotta, Katerina Machova Polakova, Thomas Ernst, Dianna Maar, Adam Corner, Michele Cavo, and Simona Soverini

Subjects
Cancer Research, Oncology, Hematology
Published
2022
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25. Alcohol dose in septal ablation for hypertrophic obstructive cardiomyopathy

Author

Veselka, Josef, primary, Faber, Lothar, additional, Liebregts, Max, additional, Cooper, Robert, additional, Januska, Jaroslav, additional, Kashtanov, Maksim, additional, Dabrowski, Maciej, additional, Hansen, Peter Riis, additional, Seggewiss, Hubert, additional, Bonaventura, Jiri, additional, Polakova, Eva, additional, Hansvenclova, Eva, additional, Bundgaard, Henning, additional, ten Berg, Jurriën, additional, Stables, Rodney Hilton, additional, Jarkovsky, Jiri, additional, and Jensen, Morten Kvistholm, additional

Published
2021
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26. Sex-Related Differences in Outcomes of Alcohol Septal Ablation for Hypertrophic Obstructive Cardiomyopathy

Author

Veselka, Josef, primary, Faber, Lothar, additional, Liebregts, Max, additional, Cooper, Robert, additional, Kashtanov, Maksim, additional, Hansen, Peter Riis, additional, Bonaventura, Jiri, additional, Polakova, Eva, additional, Hansvenclova, Eva, additional, Bundgaard, Henning, additional, ten Berg, Jurriën, additional, and Jensen, Morten Kvistholm, additional

Published
2021
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27. Immunophenotyping in pemphigus reveals a TH17/TFH17 cell–dominated immune response promoting desmoglein1/3-specific autoantibody production

Author

Holstein, Julia, primary, Solimani, Farzan, additional, Baum, Carolin, additional, Meier, Katharina, additional, Pollmann, Robert, additional, Didona, Dario, additional, Tekath, Tobias, additional, Dugas, Martin, additional, Casadei, Nicolas, additional, Hudemann, Christoph, additional, Polakova, Alexandra, additional, Matthes, Jakob, additional, Schäfer, Iris, additional, Yazdi, Amir S., additional, Eming, Rüdiger, additional, Hertl, Michael, additional, Pfützner, Wolfgang, additional, Ghoreschi, Kamran, additional, and Möbs, Christian, additional

Published
2021
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30. Fraud Detection by a Multinomial Model: Separating Honesty from Unobserved Fraud

Author

Jonas Andersson, Andreas Olden, and Aija Polakova

Subjects
Standard error, Computer science, Honesty, media_common.quotation_subject, Monte Carlo method, Expectation–maximization algorithm, Econometrics, Estimator, Suspect, Standard deviation, Multinomial logistic regression, media_common
Abstract

In this paper we investigate the EM-estimator of the model by Caudill et al. (2005). The purpose of the model is to identify items, e.g. individuals or companies, that are wrongly classified as honest; an example of this is the detection of tax evasion. Normally, we observe two groups of items, labeled fraudulent and honest, but suspect that many of the observationally honest items are, in fact, fraudulent. The items observed as honest are therefore divided into two unobserved groups, honestH, representing the truly honest, and honestF, representing the items that are observed as honest, but that are actually fraudulent. By using a multinomial logit model and assuming commonality between the observed fraudulent and the unobserved honestF, Caudill et al. (2005) present a method that uses the EM-algorithm to separate them. By means of a Monte Carlo study, we investigate how well the method performs, and under what circumstances. We also study how well bootstrapped standard errors estimates the standard deviation of the parameter estimators.

Published
2020
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31. How Far is Contract and Employee Telework Implemented in SMEs and Large Enterprises? (Case of Slovakia)

Author

Emil Wojcak, Michaela Polakova, Lubica Bajzikova, and Helena Sajgalikova

Subjects
Scheme (programming language), business.industry, 05 social sciences, Legislation, 010501 environmental sciences, Public relations, 01 natural sciences, Work (electrical), 0502 economics and business, General Materials Science, business, computer, 050203 business & management, Industrial organization, 0105 earth and related environmental sciences, Research data, computer.programming_language
Abstract

The paper deals with implementation of telework and highlights the inconsistencies in presented outcomes of various surveys focusing on numbers of organizations implementing it. These discrepancies result from differences in legislation of individual countries and differing methodologies used in individual surveys. The research data present the implementation of a particular type of telework (contract and employee telework) in Slovakia and compares the extent to which these work arrangements are implemented in SMEs and large enterprises and in the organizations classified by their ownership scheme.

Published
2016
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32. How to Achieve Sustainable Efficiency with Teleworkers: Leadership Model in Telework

Author

Emil Wojcak, Lubica Bajzikova, Michaela Polakova, and Helena Sajgalikova

Subjects
Engineering, Process management, media_common.quotation_subject, Population, 010501 environmental sciences, 01 natural sciences, Competition (economics), Globalization, 0502 economics and business, Leadership of teleworkers, Production (economics), General Materials Science, Function (engineering), education, 0105 earth and related environmental sciences, media_common, education.field_of_study, business.industry, 05 social sciences, Public relations, Identification (information), Leadership model in telework, Work (electrical), Information and Communications Technology, Motivation of teleworkers, business, 050203 business & management
Abstract

Globalization, increasing competition, changes in demographic structure of population, and development of information and communication technologies (ICT) are the factors that have posed new challenges for organizations in recent years. The newly fledged conditions have vital impact on production methods and procedures as well as work arrangements in organizations. Currently, organizations cope with various times in which work is executed as well as various places where it is executed. Thanks to ICT an employee can work at the client's place, at home, in a means of transport or in a cafe. All these situations constitute the work arrangement in which the worker is considered to work ‘from distance’, i.e. as a teleworker. The presented approach refers to the flexible work arrangement where an organization's priority lies with its endeavour to become and stay flexible in terms of time and place of performance. Under these conditions the implementation of new ways of work arrangements presents new challenges for leadership. To accomplish this basic function of management when employees are not present at workplace or even are not available during working hours and face-to-face communication is impossible poses many difficulties. The paper focuses on comparison and identification of the differences between traditional work arrangements and telework, highlights weak points in leadership of teleworkers, and proposes a leadership model for teleworkers’ induction.

Published
2016
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33. Toxicity of carbon dots – Effect of surface functionalization on the cell viability, reactive oxygen species generation and cell cycle

Author

Jiri Tucek, Martin Petr, Josef Skopalik, Markéta Havrdová, Katerina Hola, Klára Čépe, Radek Zboril, Katerina Tomankova, Katerina Polakova, and Athanasios B. Bourlinos

Subjects
Polyethylenimine, Materials science, Biocompatibility, 02 engineering and technology, General Chemistry, Cell cycle, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Biochemistry, chemistry, Quantum dot, Biophysics, Surface modification, General Materials Science, Viability assay, 0210 nano-technology, Cytotoxicity, Intracellular
Abstract

Carbon dots (CDs) are fluorescent nanoprobes offering a great potential in biological and medical applications due to their superior biocompatibility compared to metal chalcogenide quantum dots (e.g., CdSe). Key factors determining their cytotoxicity and cellular/intracellular tracking involve chemical nature and charge of surface functional groups. For the first time, we present a comprehensive cytotoxic study including cell cycle analysis of carbon dots differing in surface functionalization, namely pristine CDs (CDs-Pri) with negative charge due to carboxylic groups, polyethyleneglycol modified dots with neutral charge (CDs-PEG), and polyethylenimine coated dots with a positive charge (CDs-PEI). The CDs in vitro toxicity was studied on standard mouse fibroblasts (NIH/3T3). The results suggest that neutral CDs-PEG are the most promising for biological applications as they do not induce any abnormalities in cell morphology, intracellular trafficking, and cell cycle up to concentrations of 300 μg mL−1. Negatively charged CDs-Pri arrested the G2/M phase of the cell cycle, stimulated proliferation and led to higher oxidative stress, however they did not enter the cell nucleus. In contrast, positively charged CDs-PEI are the most cytotoxic, entering into the cell nucleus and inducing the largest changes in G0/G1 phase of cell cycle, even at concentrations of around 100 μg mL−1.

Published
2016
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35. The spectrum of brain MRI findings of methanol intoxication after the methanol affair in the Czech Republic

Author

Vaneckova, M., primary, Klempir, J., additional, Pelclova, D., additional, Bezdicek, O., additional, Liskova, I., additional, Brozova, H., additional, Polakova, K., additional, Diblik, P., additional, Miovsky, M., additional, Hubacek, J., additional, Hlusicka, J., additional, Kotikova, K., additional, Ruzicka, E., additional, Seidl, Z., additional, Marechal, B., additional, Kober, T., additional, and Zacharov, S., additional

Published
2019
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37. Does a minimal intervention approach threaten the biodiversity of protected areas? A multi-taxa short-term response to intervention in temperate oak-dominated forests

Author

Michal Platek, Filip Trnka, Jiri Benes, Martin Skorpik, Pavel Sebek, Ondrej Machac, Michael Bartoš, Radek Bače, Michal Perlík, Miroslav Svoboda, Miroslav Dvorsky, Jakub Kovar, Simona Polakova, Mojmir Vlasin, Jiri Dolezal, Michal Zapletal, Lukas Cizek, Robert Stejskal, Zuzana Chlumská, and Blanka Mikatova

Subjects
Ecology, Agroforestry, National park, Biodiversity, Forestry, Woodland, Management, Monitoring, Policy and Law, Biology, Forest restoration, Habitat, Threatened species, Secondary forest, Species richness, Nature and Landscape Conservation
Abstract

Efficient conservation management must be applied in protected areas in order to slow the loss of biodiversity in Europe. Regarding forests, a conservation approach based on minimal intervention prevails in most protected woodlands, thus facilitating the expansion of closed-canopy forests at the expense of open forests. To identify effective conservation strategies for protected forests, the minimal intervention or “hands-off” approach must be compared with active measures to support biodiversity. We carried out a study in the oak-dominated forests of Podyji National Park (Czech Republic), an historically managed area left for natural succession since 1950. Twelve experimental clearings were created in closed-canopy forests within the core zone of the park; six of these clearings were connected to forest edges and open meadows, the remaining six clearings were isolated from open habitats within closed forest. To assess the importance of minimal intervention and active management measures in protected forests, we compared the richness and composition of insects, reptiles, birds and vascular plants in the clearings and in four reference habitats, including closed forest, forest edge, open forest, and meadow, in the first season following the intervention. In comparison to closed-canopy forest, the clearings had higher species richness of butterflies, saproxylic and floricolous beetles, reptiles, and vascular plants but lower richness of moths and epigeic beetles, and similar richness of birds. For most groups, the species composition of clearings differed from that of closed forest or even the forest edge, indicating that the latter habitats cannot serve as a sufficient replacements for the conservation of open woodland species. The species richness of isolated clearings was generally lower than that of clearings connected to open habitats, and their communities contained a larger proportion of species associated with closed forest. Most threatened species were associated with clearings or open forest, closed forest and meadow hosted only a few. The creation of the clearings in closed-canopy forests had a positive effect on overall species richness and supported threatened species in most model groups. It is thus a valuable management tool in the conservation of temperate woodland biodiversity. Our results also point to the importance of connectivity of open habitats in wooded landscapes. Further surveys of the clearings are needed to ascertain the effect of such interventions to see how communities change throughout succession, or alternatively to what extent hindering succession by repeated cutting may alter communities.

Published
2015
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38. Expression of HLA-G transcripts in graft biopsy samples of renal transplant recipients

Author

Martin Chrastina, Katarina Polakova, Branislav Trebatický, Mária Pavlechová, Zuzana Žilinská, Jan Breza, Helena Bandžuchová, Martina Handzušová, Daniel Kuba, Boris Rychlý, Ivana Dedinská, and Jana Tirpáková

Subjects
Adult, Graft Rejection, Male, Pathology, medicine.medical_specialty, Biopsy, T-Lymphocytes, Immunology, Human leukocyte antigen, Andrology, medicine, Humans, Immunology and Allergy, Kidney transplantation, Aged, HLA-G Antigens, Immunity, Cellular, Transplantation, Kidney, medicine.diagnostic_test, business.industry, Panel reactive antibody, Glomerulonephritis, Middle Aged, Allografts, medicine.disease, Kidney Transplantation, Immunity, Humoral, medicine.anatomical_structure, Acute Disease, Female, Renal biopsy, business
Abstract

Background The HLA-G molecule has a high potential to modulate immune response towards the improvement of graft survival after transplantation. In this work, we have analyzed the total HLA-G mRNA expression in graft tissues of dysfunctional transplanted kidneys. Material and methods We examined 84 kidney biopsy samples obtained from 65 renal transplant recipients with dysfunctional graft (50 males, 15 females; average age 46.8 ± 11.9 years). 52 specimens were with signs of acute rejection and 32 without any rejection characteristics (diagnosed as glomerulonephritis, ATN and IFTA). Patients with acute rejection were divided into three groups: antibody-mediated rejection (AMR; n = 23), T cell-mediated rejection (TCMR; n = 16) and combined antibody and T cell-mediated rejection (AMR + TCMR; n = 13). The biopsy samples were taken from a dysfunctional graft at different time periods after kidney transplantation. The relative expression of total HLA-G mRNA in biopsy specimens was determined by real time RT-PCR. The correlation between HLA-G mRNA expression and dysfunctional graft state was investigated. The impact of different factors (post-transplantation interval, gender, mismatch, induction therapy and cold ischemia time) on relative expression of total HLA-G mRNA was also studied. Results We have found that the levels of HLA-G transcripts in kidneys with rejection were higher than those in non-rejected but dysfunctional grafts (P = 0.0003). The highest levels of HLA-G mRNA were detected at combined AMR + TCMR rejection (P = 0.005). The time-course analysis of total HLA-G mRNA expression was also studied. In both dysfunctional graft groups (rejected and non-rejected) the lower levels of HLA-G transcripts were detected during early post-transplant period (1–3 months), however a substantial increase of HLA-G mRNA expression was observed after an extended period of time (> 3 months). It was also revealed that antibody induction therapy may reduce HLA-G expression (P = 0.0004) and in female samples were higher levels of HLA-G transcripts than those in male recipients (P = 0.003). It was found no significant impact of age, cold ischemic time, PRA (Panel Reactive Antibody) score, and a number of HLA-mismatches on HLA-G mRNA expression. Conclusions We have demonstrated that the expression of total HLA-G mRNA in renal grafts can be influenced by different factors such as clinical state of transplanted kidney, elapsed time after transplantation, gender and antibody induction therapy. We have proved that HLA-G mRNA expression was significantly higher in recipients with acute rejection in comparison to patients with dysfunctional but non-rejected grafts.

Published
2015
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39. Stairway to (Secrecy) Heaven: Market Attitudes towards Secrecy Shopping

Author

Evelina Gavrilova and Aija Polakova

Subjects
Finance, business.industry, media_common.quotation_subject, Secrecy, Event study, Heaven, Sample (statistics), Business, Asset (economics), Tax planning, Tax haven, media_common
Abstract

We study asset price reactions to news on firms' decisions to acquire affiliates located in known secrecy havens. Our sample consists of data on the SP however, potential future tax planning opportunities mitigate these concerns.

Published
2018
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40. Comparison of nature-inspired population-based algorithms on continuous optimisation problems

Author

Petr Bujok, Radka Polakova, and Josef Tvrdík

Subjects
General Computer Science, Computer science, General Mathematics, Suite, 05 social sciences, 050301 education, 02 engineering and technology, Ranking (information retrieval), Random search, Dimension (vector space), Differential evolution, 0202 electrical engineering, electronic engineering, information engineering, Test suite, Benchmark (computing), 020201 artificial intelligence & image processing, Nature inspired, 0503 education, Algorithm
Abstract

Eleven swarm-intelligence-based (SI) and bio-inspired (BI) algorithms are compared with four advanced adaptive differential evolution (DE) variants, the classic DE and the blind random search on two benchmark sets. One of the benchmark sets is the CEC 2011 collection of 22 real-world optimisation problems, the latter is the suite of 30 artificial optimisation problems defined for the competition of the algorithms within CEC 2014. The results of the experiments demonstrate the superiority of the adaptive DE variants both on real-world problems and the artificial CEC 2014 test suite at all the levels of dimension (10, 30, and 50). Some of the SI and BI algorithms perform even worse than the blind random search. The efficiency of the classic DE is comparable with the better performing SI and BI methods. The results entitle to form a recommendation for practitioners: Do not propose a pseudo-new algorithm but select from the optimisation algorithms supported by thorough research and good ranking at international competitions of optimisation algorithms.

Published
2019
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41. Differential evolution with adaptive mechanism of population size according to current population diversity

Author

Petr Bujok, Radka Polakova, and Josef Tvrdík

Subjects
Mathematical optimization, Current (mathematics), General Computer Science, Computer science, Mechanism (biology), General Mathematics, Population size, 05 social sciences, 050301 education, 02 engineering and technology, Reduction (complexity), Algorithmic efficiency, Differential evolution, 0202 electrical engineering, electronic engineering, information engineering, Test suite, 020201 artificial intelligence & image processing, Adaptation, 0503 education
Abstract

A new mechanism for the adaptation of the population size in differential evolution (DE) is described. The adaptive mechanism is based on linear reduction of the population diversity and enables to decrease or increase the population size during the search. The efficiency of DE variants with and without this adaptive mechanism is experimentally compared on the CEC 2014 test suite. Moreover, this new mechanism is also compared with the linear reduction of the population size proposed in L-SHADE and used in its successful successors. The results of the comparison demonstrate the benefit from the new adaptive mechanism to the algorithm efficiency in more than a half of test problems while the deterioration of its efficiency occurs rarely. As it was expected, the use of the proposed mechanism is considerably beneficial, especially in more complex problems like multimodal, hybrid, or composition objective functions.

Published
2019
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42. The spectrum of brain MRI findings of methanol intoxication after the methanol affair in the Czech Republic

Author

Bénédicte Maréchal, J. Hlusicka, Michal Miovsky, Evzen Ruzicka, D. Pelclova, I. Liskova, J. Hubacek, P. Diblik, Tobias Kober, Jiri Klempir, S. Zacharov, Zdenek Seidl, Manuela Vaneckova, K. Polakova, K. Kotikova, Ondrej Bezdicek, and H. Brozova

Subjects
Czech, chemistry.chemical_compound, Nuclear magnetic resonance, Neurology, chemistry, business.industry, Brain mri, language, Medicine, Neurology (clinical), Methanol, business, language.human_language
Published
2019
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43. Nailing of three- and four-part fractures of the humeral head – Long-term results

Author

Karel Holub, Simona Polakova, and Martin Kloub

Subjects
Adult, Male, Humeral Fractures, medicine.medical_specialty, Adolescent, medicine.medical_treatment, Radiography, law.invention, Intramedullary rod, Postoperative Complications, law, Surveys and Questionnaires, medicine, Humans, Fractures, Comminuted, Reduction (orthopedic surgery), Aged, Retrospective Studies, General Environmental Science, Aged, 80 and over, Fracture Healing, business.industry, Incidence (epidemiology), Osteonecrosis, Retrospective cohort study, Long term results, Middle Aged, Fracture Fixation, Intramedullary, Surgery, Treatment Outcome, Cohort, Humeral Head, General Earth and Planetary Sciences, Female, Constant score, business, Follow-Up Studies
Abstract

Long term outcome of the treatment of displaced complex fractures of humeral head is rare in the literature especially in greater cohorts. Main purpose of our study was the assessment of long term results of intramedullary nailing of 3-4 part fractures.137 patients with 137 three or four-part fractures of the humeral head treated by intramedullary nailing were reassessed after an average follow-up time of 57 (27-93) months. The whole cohort consisted of 85 three-part fractures, 38 four-part fractures and 14 fracture-dislocations. Radiographic and clinical outcome - absolute Constant score (CS(abs)) and relative Constant score (compared to the contralateral side - CS(rel)) were evaluated. The rate of complications was recorded. Analysis of the influence of quality of achieved reduction on final functional result and on the development of complications was performed.125 fractures healed uneventfully. Mean long term CS(rel) was 81% of the unaffected side. Mean CS(rel) was 85% in 3-part fractures, 73% in four-part fractures, 80% in three-part fracture-dislocations and 70% in four-part fracture-dislocations. 96 (70%) patients achieved excellent or good results (CS(rel) higher than 80%), 17 (12.4%) satisfactory results and 24 (17.5%) poor results (CS(rel) less than 60%). No significant difference was observed in functional results between different age groups. No incidence of non-union was observed, 4 losses of reduction were encountered. We observed 17 cases of complete humeral head necrosis, 14 of them in the group of 4-part fractures. Reduction quality strongly influenced functional result and development of head necrosis. In the group of excellent reduction mean CS(rel) was 88% and the rate of necrosis was 2%. Moderate reduction quality deteriorated CS(rel) to 70% and head necrosis rate rose up to 28%. If reduction was poor, mean CS(rel) was 52% and the rate of complete necrosis rose to 60%.Long term results confirmed nailing as appropriate treatment strategy for all types of humeral head fractures with limitation of excellent reduction in every age group. Moderate or even poor reduction significantly deteriorates functional results and increases rate of complete necrosis of the humeral head. If good reduction cannot be achieved, treatment strategy should be changed.

Published
2014
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44. HLA-G5 in the blood of leukemia patients and healthy individuals

Author

Tatiana Železníková, Gustáv Russ, and Katarina Polakova

Subjects
Adult, Male, Cancer Research, Enzyme-Linked Immunosorbent Assay, Disease, Human leukocyte antigen, Cell Line, hemic and lymphatic diseases, Humans, Medicine, Stage (cooking), Aged, Aged, 80 and over, HLA-G Antigens, business.industry, Hematology, Middle Aged, medicine.disease, Leukemia, Lymphocytic, Chronic, B-Cell, Leukemia, Soluble hla, Oncology, Case-Control Studies, Healthy individuals, Immunology, Female, business
Abstract

In this work we focused on analysis of HLA-G5 molecules in the blood of patients with B-CLL leukemia and healthy individuals. Using sandwich ELISA, we found total soluble HLA-G, represented by sHLA-G1 and HLA-G5 in most of B-CLL patients while HLA-G5 alone was present only in few cases in both groups. These results lead us to assume that the majority of soluble HLA-G in blood is generated by proteolytic cleavage and shedding of membrane-bound HLA-G1. There is no correlation between the presence of HLA-G5 in blood of B-CLL patients and the stage of disease, age, and gender.

Published
2013
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45. Dynamics of photoinduced motions in azobenzene grafted polybutadienes

Author

Zdenka Sedlakova, Anna Kochalska, Stanislaw Bartkiewicz, L. Polakova, Stanislav Nespurek, Andrzej Miniewicz, Jaroslaw Mysliwiec, and Maciej Czajkowski

Subjects
chemistry.chemical_classification, Materials science, Organic Chemistry, Substituent, Holography, Physics::Optics, Polymer, Grating, Laser, Molecular physics, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, law.invention, Inorganic Chemistry, chemistry.chemical_compound, Photochromism, Polybutadiene, Azobenzene, chemistry, law, Polymer chemistry, Electrical and Electronic Engineering, Physical and Theoretical Chemistry, Spectroscopy
Abstract

The goal of the investigations was to measure the dynamics of photoinduced motions in semicrystalline polybutadiene backbone based polymer grafted with various degree of mesogenic thiols. We studied the influence of the grafting degree of the azobenzene-containing side groups and the length of terminal oxyaliphatic substituent in the side groups on the dynamics of the laser holographic volume and surface gratings recording. Measurements were performed in a typical laser degenerate two-wave mixing experiment, in which light self-diffraction efficiency during holographic recording was monitored. Mathematical model for grating formation based on coupling of three gratings has been assumed to elaborate the obtained results. Theoretical estimations and experimental results have shown very good agreement.

Published
2011
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46. DNA vaccine against human papillomavirus type 16: Modifications of the E6 oncogene

Author

Michal Smahel, Martina Duskova, Ingrid Polakova, and Dana Pokorná

Subjects
Recombinant Fusion Proteins, Gene Expression, Biology, Antibodies, Viral, Epitope, Cell Line, Gene gun, DNA vaccination, Mice, chemistry.chemical_compound, Neoplasms, Vaccines, DNA, Animals, Humans, Point Mutation, Papillomavirus Vaccines, Gene, Glucuronidase, Human papillomavirus 16, General Veterinary, General Immunology and Microbiology, Oncogene, Escherichia coli Proteins, Immunogenicity, Point mutation, Public Health, Environmental and Occupational Health, Oncogene Proteins, Viral, Virology, Molecular biology, Introns, Mice, Inbred C57BL, Repressor Proteins, Infectious Diseases, chemistry, Leukocytes, Mononuclear, Cytokines, Molecular Medicine, Female, DNA
Abstract

Since its discovery, DNA vaccination has become an effective strategy for the development of vaccines against cancer including cervical carcinoma (CC). The formation of CC is associated with human papillomavirus (HPV) infection. Viral E6 and E7 oncoproteins are suitable targets for therapeutic vaccination. To adapt the HPV16 E6 oncogene for DNA immunisation, we performed several modifications. First we fused the E6 gene with the 5′ or 3′-terminus of the Escherichia coli β-glucuronidase (GUS) gene and showed enhanced immunogenicity of the 3′ fusion (GUS.E6). Then, as the E6 oncogene contains two alternative introns that result in the production of truncated forms of the E6 protein, we abolished the 5′ splice site in the E6 gene. This modification completely eliminated the expression of the truncated E6 transcripts and thus increased the production of the full-length E6 protein. At the same time, it moderately reduced the immunogenicity of the modified non-fused (E6cc) or fused (GUS.E6cc) genes, probably as a consequence of the substitution in the immunodominant E6 epitope following the abolishment of the splice site. Furthermore, we reduced the oncogenicity of the E6 protein by two point mutations (E6GT) that, together, prevented E6-mediated p53 degradation. Finally, we constructed the GUS.E6GT gene characterized by enhanced safety and immunogenicity when compared with the wild-type E6 gene.

Published
2010
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49. A Second Pathway to Degrade Pyrimidine Nucleic Acid Precursors in Eukaryotes

Author

Thomas Moritz, Concetta Compagno, Jure Piškur, Annamaria Merico, Anna Rasmussen, Anders Hofer, Gorm Andersen, Olof Björnberg, Kasper Møller, Hans-Erik Åkerlund, Yuriy Pynyaha, Silvia Polakova, Zoran Gojković, and Michael P. B. Sandrini

Subjects
Pyrimidine, Respiratory chain, Biology, Fungal Proteins, Saccharomyces, chemistry.chemical_compound, Structural Biology, Urea, Lactic Acid, Pentosyltransferases, Uracil, Uridine, Molecular Biology, Gene, Uracil phosphoribosyltransferase, Molecular Structure, Nucleic Acid Precursors, Dihydrouracil, Oxygen, Metabolic pathway, Eukaryotic Cells, Pyrimidines, Biochemistry, chemistry, Mutagenesis, Site-Directed, DNA
Abstract

Pyrimidine bases are the central precursors for RNA and DNA, and their intracellular pools are determined by de novo, salvage and catabolic pathways. In eukaryotes, degradation of uracil has been believed to proceed only via the reduction to dihydrouracil. Using a yeast model, Saccharomyces kluyveri, we show that during degradation, uracil is not reduced to dihydrouracil. Six loci, named URC1-6 (for uracil catabolism), are involved in the novel catabolic pathway. Four of them, URC3,5, URC6, and URC2 encode urea amidolyase, uracil phosphoribosyltransferase, and a putative transcription factor, respectively. The gene products of URC1 and URC4 are highly conserved proteins with so far unknown functions and they are present in a variety of prokaryotes and fungi. In bacteria and in some fungi, URC1 and URC4 are linked on the genome together with the gene for uracil phosphoribosyltransferase (URC6). Urc1p and Urc4p are therefore likely the core components of this novel biochemical pathway. A combination of genetic and analytical chemistry methods demonstrates that uridine monophosphate and urea are intermediates, and 3-hydroxypropionic acid, ammonia and carbon dioxide the final products of degradation. The URC pathway does not require the presence of an active respiratory chain and is therefore different from the oxidative and rut pathways described in prokaryotes, although the latter also gives 3-hydroxypropionic acid as the end product. The genes of the URC pathway are not homologous to any of the eukaryotic or prokaryotic genes involved in pyrimidine degradation described to date.

Published
2008
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50. How did Saccharomyces evolve to become a good brewer?

Author

Jure Piškur, Elżbieta Rozpędowska, Annamaria Merico, Silvia Polakova, and Concetta Compagno

Subjects
Fermentation in winemaking, Wine, Saccharomyces cerevisiae Proteins, Ethanol, Transcription, Genetic, Saccharomyces cerevisiae, Alcohol Dehydrogenase, food and beverages, Biology, biology.organism_classification, Saccharomyces, Gene Expression Regulation, Fungal, Botany, Genetics, biology.protein, Fermentation, Ethanol fuel, Winemaking, Alcohol dehydrogenase
Abstract

Brewing and wine production are among the oldest technologies and their products are almost indispensable in our lives. The central biological agents of beer and wine fermentation are yeasts belonging to the genus Saccharomyces, which can accumulate ethanol. Recent advances in comparative genomics and bioinformatics have made it possible to elucidate when and why yeasts produce ethanol in high concentrations, and how this remarkable trait originated and developed during their evolutionary history. Two research groups have shed light on the origin of the genes encoding alcohol dehydrogenase and the process of ethanol accumulation in Saccharomyces cerevisiae.

Published
2006
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