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The objective of this study was to investigate the effects of LE fatigue on ground impact force, LE kinematics, and LE kinetics during landing. Ground reaction force (GRF), kinematic, and electromyogram (EMG) data were collected from 12 male subjects during a fatiguing landing activity (FLA). This activity allowed not only the biomechanical differences between unfatigued and fatigued landings to be determined, but also the time history of multiple biomechanical variables as fatigue progressed. EMG mean frequency analysis using data collected immediately before and after the FLA indicated that subjects experienced fatigue of the quadriceps muscles. Results indicated a decrease in ground impact force and an increase in maximum joint flexion during landing with fatigue. Joint impulse values were consistent with a distal to proximal redistribution of extensor moment production. Potential reasons for this redistribution are discussed. A trend reversal in hip and ankle impulse during the activity suggest a change in landing strategy as fatigue progressed. The data also suggest that the measured changes in landing biomechanics may have been influenced by other factors, in addition to fatigue, such as a neuromuscular protective mechanism to decrease impact force magnitude.