1. Annona muricata Linn. leaf as a source of antioxidant compounds with in vitro antidiabetic and inhibitory potential against α-amylase, α-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation
- Author
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Allisson Benatti Justino, Foued Salmen Espindola, Neide M. Silva, Natália Carnevalli Miranda, Mário Machado Martins, and Rodrigo Rodrigues Franco
- Subjects
Glycation End Products, Advanced ,Male ,0301 basic medicine ,Antioxidant ,DPPH ,medicine.medical_treatment ,Annona ,Antioxidants ,Lipid peroxidation ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Glycation ,medicine ,Animals ,Hypoglycemic Agents ,Food science ,Amylase ,Enzyme Inhibitors ,Rats, Wistar ,Annona muricata ,Pharmacology ,biology ,Plant Extracts ,Serum Albumin, Bovine ,alpha-Glucosidases ,Lipase ,General Medicine ,biology.organism_classification ,Plant Leaves ,030104 developmental biology ,chemistry ,Phytochemical ,030220 oncology & carcinogenesis ,NIH 3T3 Cells ,biology.protein ,Lipid Peroxidation ,Trolox ,alpha-Amylases - Abstract
Annona muricata leaves are used in traditional medicine to manage diabetes mellitus and its complications. The aim of this study was to evaluate the potential in vitro antidiabetic properties of Annona muricata leaf by identifying its main phytochemical constituents and characterizing the phenolic-enriched fractions for their in vitro antioxidant capacity and inhibitory activities against glycoside and lipid hydrolases, advanced glycation end-product formation and lipid peroxidation. Ethanol extract of A. muricata leaf was subjected to a liquid-liquid partitioning and its fractions were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC, FRAP and Fe2+-ascorbate-induced lipid peroxidation assays) and anti-glycation (BSA-fructose, BSA-methylglyoxal and arginine-methylglyoxal models) capacities. In addition, identification of the main bioactive compounds of A. muricata leaf by HPLC-ESI-MS/MS analysis was carried out. Ethyl acetate (EtOAc) and n-butanol (BuOH) fractions showed, respectively, antioxidant properties (ORAC 3964 ± 53 and 2707 ± 519 μmol trolox eq g−1, FRAP 705 ± 35 and 289 ± 18 μmol trolox eq g−1, and DPPH IC50 4.3 ± 0.7 and 9.3 ± 0.8 μg mL−1) and capacity to reduce liver lipid peroxidation (p
- Published
- 2018