1. Surveillance of dengue and chikungunya infection in Dong Thap, Vietnam: A 13-month study
- Author
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Vu Trong Duoc, Truong Ba Tang, Pham Thi Lien, Phan Thi Nga, Emmanuel Cornillot, Bui Minh Trang, Tran Nhu Duong, Laurent Gavotte, Roger Frutos, Laurence Briant, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Shanghai Maritime University, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Vaccination Antiparasitaire : Laboratoire de Biologie Cellulaire et Moléculaire (LBCM), Université Montpellier 1 (UM1)-Université de Montpellier (UM), RIKEN Center for Life Science Technologies (RIKEN CLST), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), National Institute of Hygiene and Epidemiology [Hanoi, Vietnam] (NIHE), and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
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0301 basic medicine ,Serotype ,Identification ,L73 - Maladies des animaux ,Virus de chikungunya ,medicine.disease_cause ,Serology ,Dengue fever ,Dengue ,0302 clinical medicine ,Medicine ,Chikungunya ,ComputingMilieux_MISCELLANEOUS ,Arbovirus ,Medicine(all) ,biology ,virus diseases ,General Medicine ,3. Good health ,Flavivirus ,Vietnam ,S50 - Santé humaine ,Cohort ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,030106 microbiology ,030231 tropical medicine ,Dong Thap ,Sérologie ,03 medical and health sciences ,Maladie de l'homme ,Multiplex polymerase chain reaction ,Surveillance épidémiologique ,business.industry ,biology.organism_classification ,medicine.disease ,Virology ,Enquête pathologique ,business ,Virose - Abstract
Objective To establish a surveillance in Dong Thap, at the border with Cambodia by assessing the presence of DENV serotypes and CHIKV among patients hospitalized at Dong Thap general hospital. Methods Cross-sectional descriptive analysis was conducted on a cohort of 131 patients hospitalized with acute fever and symptoms compatible with dengue or chikungunya. The study was conducted from January 2012 to February 2013. The full clinical picture was established as well as serological and molecular detection. Serological analysis was sequentially performed on blood samples collected on admission and an average of seven days after admission. The detection of IgM antibody to DENV was performed by IgM capture ELISA and the detection of DENV and CHIKV RNA was done by reverse-transcription multiplex PCR. Results 101 patients out of 131 (77%) were confirmed with dengue. All four dengue serotypes were detected with a predominance of DENV2 and DENV4. No chikungunya infection was detected although reported in neighboring Cambodia. A differential efficiency of serological dengue detection was observed. Efficiency was 29% upon admission and 53% after seven days on the same patients. 30 patients out of 131 (23%) were negative with both DENV and CHIKV. Conclusions Dengue is at risk of being underestimated and chikungunya is not systematically detected. Changes in detection and surveillance procedures are therefore discussed to increase efficiency of dengue detection and continue the monitoring the emergence of CHIKV in Dong Thap province and in Vietnam.
- Published
- 2016
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