1. Porcine lactoferrin administration enhances peripheral lymphocyte proliferation and assists infectious bursal disease vaccination in native chickens
- Author
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Hsiao-Ling Chen, Cheng-Wei Lai, Chia-Chou Yeh, Hsu-Chen Cheng, Willie Lin, Che-Ming Hung, Chuan-Mu Chen, Meng-Fu Kuo, Ming-Hsien Yeh, and Ming-Yu Tu
- Subjects
Interleukin 2 ,T cell ,Lymphocyte ,Administration, Oral ,Lymphocyte proliferation ,Infectious bursal disease virus ,Infectious bursal disease ,Adjuvants, Immunologic ,medicine ,Animals ,Lymphocytes ,Cells, Cultured ,Poultry Diseases ,Cell Proliferation ,General Veterinary ,General Immunology and Microbiology ,biology ,Lactoferrin ,Gene Expression Profiling ,Vaccination ,Public Health, Environmental and Occupational Health ,Antibody titer ,Viral Vaccines ,Birnaviridae Infections ,medicine.disease ,Recombinant Proteins ,Infectious Diseases ,medicine.anatomical_structure ,Immunology ,biology.protein ,Cytokines ,Molecular Medicine ,Chickens ,medicine.drug - Abstract
The purpose of this study was to investigate the effects of dietary supplementation with recombinant porcine lactoferrin (rPLF) produced by yeast culture on peripheral lymphocyte proliferation and serum antibody titers in chickens vaccinated against the infectious bursal disease (IBD) virus. Treatment groups were fed with rPLF powder in their diet (2.0%, w/w), and the IBD vaccine was administrated at 1 and 3 weeks of age. At 8, 12, and 16 weeks after vaccination, serum IBD antibody titers were measured via the micro-method and T cell proliferation rates were evaluated. In gene expression analyses, rPLF-treated chicken peripheral T lymphocytes were stimulated with concanavalin A (ConA) for 24h. The mRNA expression levels of interleukin-2 (IL-2), interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and interleukin-12 (IL-12) were determined using a semi-quantitative RT-PCR assay. The results revealed that the rPLF additive led to significant increases in serum IgG and IBD-specific antibody titers (P
- Published
- 2010
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