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MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-543 is up-regulated in gefitinib-resistant non-small cell lung cancer (NSCLC) patients comparing gefitinib-sensitive ones. It promotes NSCLC cell proliferation by negatively regulates its target gene PTEN. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-543 mimics. Transwell assay showed that miR-543 mimics promotes the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-543 directly binds to the 3'untranslated region of PTEN, and western blotting showed that miR-543 suppresses the expression of PTEN at the protein level. This study indicates that miR-543 promtes proliferation and invasion of NSCLC cell lines by PTEN. The miR-543 may represent a potential therapeutic target for gefitinib-resistant NSCLC intervention.

CO adsorption on TM-doped magnesia nanotubes (TM = Ni, Pd and Pt) have been studied by using density functional theory. Our calculation results show that CO favors adsorption on TM-doped magnesia nanotubes in the form of C atom bonding with TM atom. Fukui indices analysis clearly exhibits that doping of impurity TM atom allows for a noticeably enhancement of nucleophilic reactivity ability of magnesia nanotube. The adsorption energies demonstrate that CO molecule is more strongly bound on the 3-fold TM atoms than the 4-fold TM atoms. This finding is well confirmed by TM–C bond length, charge transfer and C–O vibrational frequency. The high adsorption energy of 2.55 eV is found when CO adsorbs on 3-fold Pt in Pt-doped magnesia nanotubes, implying the kind of the doping TM atom has a significant influence on the chemical reactivity.

Bacterial communities in decaying sapwood and heartwood of Keteleeria evelyniana were demonstrated through constructing rRNA gene libraries. At 3% of sequence difference, 81 operational taxonomic units (OTUs) were identified from 91 partial 16S rRNA gene fragments. These clones belonged to Proteobacteria (63.7%), Actinobacteria (13.2%), Acidobacteria (8.8%), Firmicutes (3.3%), Chlamydiae (5.5%), Chloiroflexi (3.3%), Verrucomicrobia (1.1%) and Cyanobacteria (1.1%). The heartwood displayed higher species richness than the sapwood. No OTU overlapped between these two parts. Most α- and β-, and all δ-proteobacterial clones occurred in the heartwood, whereas γ-proteobacteria mainly occurred in the sapwood. Chlamydiae and Verrucomicrobia exclusively appeared in the heartwood, but Chloroflexi, Cyanobacteria, and Firmicutes, distributed in the sapwood. Clones belonging to Acidobacteria and Actinobacteria were also divided into distinct subgroups between in the sapwood and in the heartwood. Based on the present species definition, we estimated the wood-inhibiting bacteria of K. evelyniana achieved up 1056 species (ranging from 452 to 2647). Only small number of sequences (13/91) can be found the nearest neighbor previously cultured and named; thereby, the majority of wood-inhabiting bacteria still remains to be uncovered.